The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty , Tol2 , and Mos1 in mammalian cells
Open accessAbstract
A nonviral vector for highly efficient site-specific integration would be desirable for many applications in transgenesis, including gene therapy. In this study we directly compared the genomic integration efficiencies of piggyBac , hyperactive Sleeping Beauty ( SB11 ), Tol2 , and Mos1 in four mammalian cell lines. piggyBac demonstrated significantly higher transposition activity in all cell lines whereas Mos1 had no activity. Furthermore, piggyBac transposase coupled to the GAL4 DNA-binding domain retains transposition activity whereas similarly manipulated gene products of Tol2 and SB11 were inactive. The high transposition activity of piggyBac and the flexibility for molecular modification of its transposase suggest the possibility of using it routinely for mammalian transgenesis.
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Cites
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Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice
Ding, S., Wu, X., Li, G., Han, M., Zhuang, Y., & Xu, T. (2005) · Cell · doi:10.1016/j.cell.2005.07.013 · Cited by 1,009
Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system
Dupuy, A. J., Akagi, K., Largaespada, D. A., Copeland, N. G., & Jenkins, N. A. (2005) · Nature · doi:10.1038/nature03691 · Cited by 482
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Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse
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Gene transfer into genomes of human cells by the sleeping beauty transposon system
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High-Resolution Genome-Wide Mapping of Transposon Integration in Mammals
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Cited by
Non-viral vectors for gene-based therapy
Yin, H., Kanasty, R. L., Eltoukhy, A. A., Vegas, A. J., Dorkin, J. R., & Anderson, D. G. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3763 · Cited by 3,123
A hyperactive piggyBac transposase for mammalian applications
Yusa, K., Zhou, L., Li, M. A., Bradley, A., & Craig, N. L. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1008322108 · Cited by 770
Tol2: a versatile gene transfer vector in vertebrates
Kawakami, K. (2007) · Genome Biology · doi:10.1186/gb-2007-8-s1-s7 · Cited by 566
DNA Transposons: Nature and Applications in Genomics
Munoz-Lopez, M., & Garcia-Perez, J. (2010) · Current Genomics · doi:10.2174/138920210790886871 · Cited by 447
Building a lineage from single cells: genetic techniques for cell lineage tracking
Woodworth, M. B., Girskis, K. M., & Walsh, C. A. (2017) · Nature Reviews Genetics · doi:10.1038/nrg.2016.159 · Cited by 236
Metadata
- Authors
- Wu, S. C.-Y., Meir, Y.-J. J., Coates, C. J., Handler, A. M., Pelczar, P., Moisyadi, S., & Kaminski, J. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-10-10
- Volume
- 103
- Issue
- 41
- Pages
- 15008-15013
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2074679317
- Cited by
- 353
- References
- 26
- FWCI
- 7.86
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Wu, S. C.-Y., Meir, Y.-J. J., Coates, C. J., Handler, A. M., Pelczar, P., Moisyadi, S., & Kaminski, J. M. (2006). piggyBac is a flexible and highly active transposon as compared to Sleeping Beauty , Tol2 , and Mos1 in mammalian cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0606979103
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