The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Endogenous oxidative stress produces diversity and adaptability in biofilm communities
Open accessAbstract
Many bacterial species are capable of biofilm growth, in which cells live and replicate within multicellular community groups. Recent work shows that biofilm growth by a wide variety of bacterial species can generate genetic diversity in microbial populations. This finding is significant because the presence of diverse subpopulations can extend the range of conditions in which communities can thrive. Here, we used biofilms formed by the pathogen Pseudomonas aeruginosa to investigate how this population diversity is produced. We found that some cells within biofilms incur double-stranded DNA breaks caused by endogenous oxidative stress. Genetic variants then result when breaks are repaired by a mutagenic mechanism involving recombinatorial DNA repair genes. We hypothesized that the mutations produced could promote the adaptation of biofilm communities to changing conditions in addition to generating diversity. To test this idea, we exposed biofilms to an antibiotic and found that the oxidative stress-break repair mechanism increased the emergence of antibiotic-resistant bacteria. The diversity and adaptability produced by this mechanism could help biofilm communities survive in harsh environments.
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Self-citationThe contribution of melanin to microbial pathogenesis: Melanin and microbial pathogenesis
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Cited by
Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence
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(2012) · doi:10.1007/978-94-007-3855-3 · Cited by 1,078
Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms
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Environmental factors that shape biofilm formation
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Filamentous Bacteriophage Promote Biofilm Assembly and Function
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Self-citation
Metadata
- Authors
- Boles, B. R., & Singh, P. K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2008-08-26
- Volume
- 105
- Issue
- 34
- Pages
- 12503-12508
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2079542855
- Cited by
- 353
- References
- 36
- FWCI
- 6.58
- Type
- journal article
- Language
- en
- Open access
- green
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