The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases
Open accessAbstract
We report very high gene targeting frequencies in Drosophila by direct embryo injection of mRNAs encoding specific zinc-finger nucleases (ZFNs). Both local mutagenesis via nonhomologous end joining (NHEJ) and targeted gene replacement via homologous recombination (HR) have been achieved in up to 10% of all targets at a given locus. In embryos that are wild type for DNA repair, the products are dominated by NHEJ mutations. In recipients deficient in the NHEJ component, DNA ligase IV, the majority of products arise by HR with a coinjected donor DNA, with no loss of overall efficiency in target modification. We describe the application of the ZFN injection procedure to mutagenesis by NHEJ of 2 new genes in Drosophila melanogaster : coil and pask . Pairs of novel ZFNs designed for targets within those genes led to the production of null mutations at each locus. The injection procedure is much more rapid than earlier approaches and makes possible the generation and recovery of targeted gene alterations at essentially any locus within 2 fly generations.
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Cites
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Highly efficient endogenous human gene correction using designed zinc-finger nucleases
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An improved zinc-finger nuclease architecture for highly specific genome editing
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Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases
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Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
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Cited by
Genome editing with engineered zinc finger nucleases
Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S., & Gregory, P. D. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2842 · Cited by 2,285
Therapeutic genome editing: prospects and challenges
Cox, D. B. T., Platt, R. J., & Zhang, F. (2015) · Nature Medicine · doi:10.1038/nm.3793 · Cited by 1,336
Genome Engineering With Zinc-Finger Nucleases
Carroll, D. (2011) · Genetics · doi:10.1534/genetics.111.131433 · Cited by 1,076
Self-citationOptimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila
Port, F., Chen, H.-M., Lee, T., & Bullock, S. L. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1405500111 · Cited by 1,046
Accurate classification of BRCA1 variants with saturation genome editing
Findlay, G. M., Daza, R. M., Martin, B., Zhang, M. D., Leith, A. P., Gasperini, M., Janizek, J. D., Huang, X., Starita, L. M., & Shendure, J. (2018) · Nature · doi:10.1038/s41586-018-0461-z · Cited by 843
Metadata
- Authors
- Beumer, K. J., Trautman, J. K., Bozas, A., Liu, J.-L., Rutter, J., Gall, J. G., & Carroll, D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2008-12-16
- Volume
- 105
- Issue
- 50
- Pages
- 19821-19826
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1972209711
- Cited by
- 281
- References
- 50
- FWCI
- 6.33
- Type
- journal article
- Language
- en
- Open access
- green
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- Beumer, K. J., Trautman, J. K., Bozas, A., Liu, J.-L., Rutter, J., Gall, J. G., & Carroll, D. (2008). Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0810475105
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