Influences of aging and caloric restriction on the transcriptional profile of skeletal muscle from rhesus monkeys
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In laboratory rodents, caloric restriction (CR) retards several age-dependent physiological and biochemical changes in skeletal muscle, including increased steady-state levels of oxidative damage to lipids, DNA, and proteins. We have previously used high-density oligonucleotide arrays to show that CR can prevent or delay most of the major age-related transcriptional alterations in the gastrocnemius muscle of C57BL/6 mice. Here we report the effects of aging and adult-onset CR on the gene expression profile of 7,070 genes in the vastus lateralis muscle from rhesus monkeys. Gene expression analysis of aged rhesus monkeys (mean age of 26 years) was compared with that of young animals (mean age of 8 years). Aging resulted in a selective up-regulation of transcripts involved in inflammation and oxidative stress, and a down-regulation of genes involved in mitochondrial electron transport and oxidative phosphorylation. Middle-aged monkeys (mean age of 20 years) subjected to CR since early adulthood (mean age of 11 years) were studied to determine the gene expression profile induced by CR. CR resulted in an up-regulation of cytoskeletal protein-encoding genes, and also a decrease in the expression of genes involved in mitochondrial bioenergetics. Surprisingly, we did not observe any evidence for an inhibitory effect of adult-onset CR on age-related changes in gene expression. These results indicate that the induction of an oxidative stress-induced transcriptional response may be a common feature of aging in skeletal muscle of rodents and primates, but the extent to which CR modifies these responses may be species-specific.
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Skeletal muscle from aged humans, monkeys, and mice demonstrates elevated levels of several senescence associated genes including p53, p21, and Gadd45a.
“skeletal muscle from aged humans, monkeys, and mice demonstrates elevated levels of several senescence associated genes including p53, p21, and Gadd45a”
From Skeletal muscle aging, cellular senescence, and senotherapeutics: current knowledge and future directions (2021) · cites this paper as Kayo et al., 2001
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Self-citation
- Authors
- Kayo, T., Allison, D. B., Weindruch, R., & Prolla, T. A.
- Affiliations
- St. Luke's-Roosevelt Hospital Center (US)
- Corresponding author
- Weindruch, Richard
- ORCID
- Allison, David B.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2001-04-17
- Volume
- 98
- Issue
- 9
- Pages
- 5093-5098
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC33169
- OpenAlex
- W2073151196
- Cited by
- 384
- References
- 26
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 7 · 2023: 7 · 2022: 2 · 2021: 7 · 2020: 3 · 2019: 6 · 2018: 5 · 2017: 4 · 2016: 7 · 2015: 10 · 2014: 8 · 2013: 8 · 2012: 19
- FWCI
- 11.31
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Aging (AG 11915, P01AG011915)
- National Cancer Institute (R01 CA 78732)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- caloric restriction, aging, skeletal muscle, gene expression profiles, rhesus monkeys, oxidative stress, inflammation, mitochondrial electron transport chain, oxidative phosphorylation, cytoskeletal proteins, mitochondrial bioenergetics, species differences, gastrocnemius, vastus lateralis
- MeSH terms
- Aging, Animals, Energy Intake, Electron Transport, Food Deprivation, Humans, Macaca mulatta, Male, Oxidative Phosphorylation, RNA, Messenger, Transcription, Genetic, Down-Regulation, Up-Regulation, Oxidative Stress, Muscle, Skeletal, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling
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