DNA microarray analysis of the aging brain
Abstract
To examine molecular events associated with brain aging and its retardation by caloric restriction (CR), we have employed high-density oligonucleotide arrays providing data on 6347 genes to define transcriptional patterns in two brain regions (cerebellum and neocortex). Male C57BL/6 mice were either fed normally or subjected to CR. To investigate aging, 5 month (young adult) and 30 month-old normally fed mice were compared. To study CR, 30 month-old control and CR mice were compared. In both brain regions, aging resulted in a gene expression profile suggestive of a marked inflammatory response, oxidative stress and reduced neuronal plasticity and neurotrophic support. In the brain, CR selectively attenuated the age-associated induction of genes encoding inflammatory and stress responses. In addition to providing an improved understanding of the aging process, the use of DNA microarrays generates panels of hundreds of transcriptional biomarkers of molecular aging, providing a new tool to measure biological age on a tissue-specific basis. These studies suggest that genomic approaches may be useful in understanding the molecular basis of the aging process in experimental animals.
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Accumulated pro-inflammation and oxidative stress in the brain, often observed in aged brains and aged microglia, could cause sustained suppression of Trem2 expression and thus an impairment of TREM2-dependent microglial functions.
“accumulated pro-inflammation and oxidative stress in the brain, which is often observed in aged brains and aged microglia (90–94), could cause sustained suppression of Trem2 expression and thus an impairment of TREM2-dependent microglial functions.”
From <i>Trem2</i> promotes anti-inflammatory responses in microglia and is suppressed under pro-inflammatory conditions (2020) · cites this paper as [90]
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- Authors
- Prolla, T. A.
- Affiliations
- University of Wisconsin–Madison (US)
- Corresponding author
- Prolla, T. A.
- Venue
- Chemical Senses
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-03-01
- Volume
- 27
- Issue
- 3
- Pages
- 299-306
- ISSN
- 0379-864X (ISSN-L)
- 1464-3553
- OpenAlex
- W2158838681
- Cited by
- 110
- References
- 27
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 7 · 2019: 3 · 2018: 2 · 2017: 4 · 2016: 6 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 7
- FWCI
- 3.07
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Chemical Senses (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ca078723-04)
- National Cancer Institute (R01 CA 78723)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Antioxidants, Aging, Portulaca oleracea
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- brain aging, caloric restriction, gene expression profiling, cerebellum, neocortex, neuroinflammation, oxidative stress, neuroplasticity, neurotrophic support, biological age, C57BL/6 mice
- MeSH terms
- Aging, Animals, Brain, Macaca mulatta, Male, Mice, Inbred C57BL, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Mice
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