The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome
Open accessAbstract
We previously reported assembly and cloning of the synthetic Mycoplasma genitalium JCVI-1.0 genome in the yeast Saccharomyces cerevisiae by recombination of six overlapping DNA fragments to produce a 592-kb circle. Here we extend this approach by demonstrating assembly of the synthetic genome from 25 overlapping fragments in a single step. The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
An introduction to probability theory and its applications
(1958) · Journal of the Franklin Institute · doi:10.1016/0016-0032(58)90688-4 · Cited by 29,269
Hinnen, A., Hicks, J. B., & Fink, G. R. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.4.1929 · Cited by 2,205
Yeast transformation: a model system for the study of recombination.
Orr-Weaver, T. L., Szostak, J. W., & Rothstein, R. J. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.10.6354 · Cited by 1,455
Complete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome
Gibson, D. G., Benders, G. A., Andrews-Pfannkoch, C., Denisova, E. A., Baden-Tillson, H., Zaveri, J., Stockwell, T. B., Brownley, A., Thomas, D. W., Algire, M. A., et al. (2008) · Science · doi:10.1126/science.1151721 · Cited by 1,243
Self-citationPlasmid construction by homologous recombination in yeast
Ma, H., Kunes, S., Schatz, P. J., & Botstein, D. (1987) · Gene · doi:10.1016/0378-1119(87)90376-3 · Cited by 560
An Introduction to Probability Theory and its Applications.
David, F. N., & Feller, W. (1958) · Biometrika · doi:10.2307/2333079 · Cited by 465
Nonhomologous End Joining in Yeast
Daley, J. M., Palmbos, P. L., Wu, D., & Wilson, T. E. (2005) · Annual Review of Genetics · doi:10.1146/annurev.genet.39.073003.113340 · Cited by 415
Kouprina, N., & Larionov, V. (2008) · Nature Protocols · doi:10.1038/nprot.2008.5 · Cited by 233
Itaya, M., Tsuge, K., Koizumi, M., & Fujita, K. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0503868102 · Cited by 191
General Method for Plasmid Construction Using Homologous Recombination
Raymond, C. K., Pownder, T. A., & Sexson, S. L. (1999) · BioTechniques · doi:10.2144/99261rr02 · Cited by 160
Cited by
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Gibson, D. G., Glass, J. I., Lartigue, C., Noskov, V. N., Chuang, R.-Y., Algire, M. A., Benders, G. A., Montague, M. G., Ma, L., Moodie, M. M., et al. (2010) · Science · doi:10.1126/science.1190719 · Cited by 2,638
Self-citationDesign and synthesis of a minimal bacterial genome
Hutchison, C. A., Chuang, R.-Y., Noskov, V. N., Assad-Garcia, N., Deerinck, T. J., Ellisman, M. H., Gill, J., Kannan, K., Karas, B. J., Ma, L., et al. (2016) · Science · doi:10.1126/science.aad6253 · Cited by 1,500
Self-citationLarge-scale de novo DNA synthesis: technologies and applications
Kosuri, S., & Church, G. M. (2014) · Nature Methods · doi:10.1038/nmeth.2918 · Cited by 869
A Synthetic Genetic Edge Detection Program
Tabor, J. J., Salis, H. M., Simpson, Z. B., Chevalier, A. A., Levskaya, A., Marcotte, E. M., Voigt, C. A., & Ellington, A. D. (2009) · Cell · doi:10.1016/j.cell.2009.04.048 · Cited by 488
Synthetic chromosome arms function in yeast and generate phenotypic diversity by design
Dymond, J. S., Richardson, S. M., Coombes, C. E., Babatz, T., Muller, H., Annaluru, N., Blake, W. J., Schwerzmann, J. W., Dai, J., Lindstrom, D. L., et al. (2011) · Nature · doi:10.1038/nature10403 · Cited by 473
Metadata
- Authors
- Gibson, D. G., Benders, G. A., Axelrod, K. C., Zaveri, J., Algire, M. A., Moodie, M., Montague, M. G., Venter, J. C., Smith, H. O., & Hutchison, C. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2008-12-23
- Volume
- 105
- Issue
- 51
- Pages
- 20404-20409
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2062736124
- Cited by
- 477
- References
- 18
- FWCI
- 9.31
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome. https://app.cochat.ai/papers/10.1073/pnas.0811011106
- The paper
- Gibson, D. G., Benders, G. A., Axelrod, K. C., Zaveri, J., Algire, M. A., Moodie, M., Montague, M. G., Venter, J. C., Smith, H. O., & Hutchison, C. A. (2008). One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0811011106
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
