Cited by 261Escherichia coli research studiesvaccines and immunoinformatics approachesMicrobial infections and disease research

Identification of protective and broadly conserved vaccine antigens from the genome of extraintestinal pathogenic Escherichia coli

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Moriel, Danilo Gomes, Bertoldi, Isabella, Spagnuolo, Angela, Marchi, Sara, Rosini, Roberto, Nesta, Barbara, Pastorello, Ilaria, Corea, Vanja A. Mariani +17 more·2010·Proceedings of the National Academy of Sciences

Abstract

Extraintestinal pathogenic Escherichia coli (ExPEC) are a common cause of disease in both mammals and birds. A vaccine to prevent such infections would be desirable given the increasing antibiotic resistance of these bacteria. We have determined the genome sequence of ExPEC IHE3034 (ST95) isolated from a case of neonatal meningitis and compared this to available genome sequences of other ExPEC strains and a few nonpathogenic E. coli . We found 19 genomic islands present in the genome of IHE3034, which are absent in the nonpathogenic E. coli isolates. By using subtractive reverse vaccinology we identified 230 antigens present in ExPEC but absent (or present with low similarity) in nonpathogenic strains. Nine antigens were protective in a mouse challenge model. Some of them were also present in other pathogenic non-ExPEC strains, suggesting that a broadly protective E. coli vaccine may be possible. The gene encoding the most protective antigen was detected in most of the E. coli isolates, highly conserved in sequence and found to be exported by a type II secretion system which seems to be nonfunctional in nonpathogenic strains.

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Authors
Moriel, D. G., Bertoldi, I., Spagnuolo, A., Marchi, S., Rosini, R., Nesta, B., Pastorello, I., Corea, V. A. M., Torricelli, G., Cartocci, E., Savino, S., Scarselli, M., Dobrindt, U., Hacker, J., Tettelin, H., Tallon, L. J., Sullivan, S., Wieler, L. H., Ewers, C., Pickard, D., Dougan, G., Fontana, M. R., Rappuoli, R., Pizza, M., & Serino, L.
Affiliations
  • Novartis (Italy) (IT)
  • University of Würzburg (DE)
  • University of Maryland, Baltimore (US)
  • J. Craig Venter Institute (US)
  • Freie Universität Berlin (DE)
  • Wellcome Sanger Institute (GB)
  • Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK
Venue
Proceedings of the National Academy of Sciences
Published
2010-05-03
Volume
107
Issue
20
Pages
9072-9077
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W1972223538
Cited by
261
References
54
Citations by year
2026: 8 · 2025: 12 · 2024: 13 · 2023: 13 · 2022: 4 · 2021: 15 · 2020: 18 · 2019: 10 · 2018: 20 · 2017: 10 · 2016: 28 · 2015: 19 · 2014: 20 · 2013: 28 · 2012: 26
FWCI
15.11
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Wellcome Trust
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • Bundesministerium für Bildung und Forschung (01KI07120)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
  • Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Keywords
extraintestinal pathogenic Escherichia coli, vaccine antigens, genomic islands, neonatal meningitis, ST95, type II secretion system, conserved antigens, mouse challenge protection, genome sequence
MeSH terms
Animals, Antigens, Bacterial, Base Sequence, Escherichia coli, Escherichia coli Infections, Finland, Humans, Molecular Sequence Data, Genome, Bacterial, Sequence Analysis, DNA, Computational Biology, Meningitis, Escherichia coli, Escherichia coli Vaccines, Genomic Islands, Mice, Secretory Pathway

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