The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Bacteria-induced natural product formation in the fungus Aspergillus nidulans requires Saga/Ada-mediated histone acetylation
Open accessAbstract
Sequence analyses of fungal genomes have revealed that the potential of fungi to produce secondary metabolites is greatly underestimated. In fact, most gene clusters coding for the biosynthesis of antibiotics, toxins, or pigments are silent under standard laboratory conditions. Hence, it is one of the major challenges in microbiology to uncover the mechanisms required for pathway activation. Recently, we discovered that intimate physical interaction of the important model fungus Aspergillus nidulans with the soil-dwelling bacterium Streptomyces rapamycinicus specifically activated silent fungal secondary metabolism genes, resulting in the production of the archetypal polyketide orsellinic acid and its derivatives. Here, we report that the streptomycete triggers modification of fungal histones. Deletion analysis of 36 of 40 acetyltransferases, including histone acetyltransferases (HATs) of A. nidulans , demonstrated that the Saga/Ada complex containing the HAT GcnE and the AdaB protein is required for induction of the orsellinic acid gene cluster by the bacterium. We also showed that Saga/Ada plays a major role for specific induction of other biosynthesis gene clusters, such as sterigmatocystin, terrequinone, and penicillin. Chromatin immunoprecipitation showed that the Saga/Ada-dependent increase of histone 3 acetylation at lysine 9 and 14 occurs during interaction of fungus and bacterium. Furthermore, the production of secondary metabolites in A. nidulans is accompanied by a global increase in H3K14 acetylation. Increased H3K9 acetylation, however, was only found within gene clusters. This report provides previously undescribed evidence of Saga/Ada dependent histone acetylation triggered by prokaryotes.
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Cites
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New nomenclature for chromatin-modifying enzymes
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LaeA, a Regulator of Secondary Metabolism in Aspergillus spp
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Fusion PCR and gene targeting in Aspergillus nidulans
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Fungal secondary metabolites – Strategies to activate silent gene clusters
Brakhage, A. A., & Schroeckh, V. (2011) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2010.04.004 · Cited by 729
Self-citationSchroeckh, V., Scherlach, K., Nützmann, H.-W., Shelest, E., Schmidt-Heck, W., Schuemann, J., Martin, K., Hertweck, C., & Brakhage, A. A. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0901870106 · Cited by 663
Self-citationGenomics-driven discovery of PKS-NRPS hybrid metabolites from Aspergillus nidulans
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Self-citationTriggering cryptic natural product biosynthesis in microorganisms
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Self-citationRegulation of Secondary Metabolism in Filamentous Fungi
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Self-citationGacek-Matthews, A., Berger, H., Sasaki, T., Wittstein, K., Gruber, C., Lewis, Z. A., & Strauss, J. (2016) · PLOS Genetics · doi:10.1371/journal.pgen.1006222 · Cited by 105
Self-citationCánovas, D., Marcos, A. T., Gacek, A., Ramos, M. S., Gutiérrez, G., Reyes-Domínguez, Y., & Strauss, J. (2014) · Genetics · doi:10.1534/genetics.114.165688 · Cited by 75
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Metadata
- Authors
- Nützmann, H.-W., Reyes-Dominguez, Y., Scherlach, K., Schroeckh, V., Horn, F., Gacek, A., Schümann, J., Hertweck, C., Strauss, J., & Brakhage, A. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2011-08-08
- Volume
- 108
- Issue
- 34
- Pages
- 14282-14287
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2029783392
- Cited by
- 337
- References
- 38
- FWCI
- 16.69
- Type
- journal article
- Language
- en
- Open access
- green
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