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Cytoskeletal microdifferentiation: a mechanism for organizing morphological plasticity in dendrites

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Kaech, Stefanie, Parmar, Hema, Roelandse, Martijn, Bornmann, Caroline, Matus, Andrew·2001·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.111146798PMC PMC34627
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Abstract

Experimental evidence suggests that microfilaments and microtubules play contrasting roles in regulating the balance between motility and stability in neuronal structures. Actin-containing microfilaments are associated with structural plasticity, both during development when their dynamic activity drives the exploratory activity of growth cones and after circuit formation when the actin-rich dendritic spines of excitatory synapses retain a capacity for rapid changes in morphology. By contrast, microtubules predominate in axonal and dendritic processes, which appear to be morphologically relatively more stable. To compare the cytoplasmic distributions and dynamics of microfilaments and microtubules we made time-lapse recordings of actin or the microtubule-associated protein 2 tagged with green fluorescent protein in neurons growing in dispersed culture or in tissue slices from transgenic mice. The results complement existing evidence indicating that the high concentrations of actin present in dendritic spines is a specialization for morphological plasticity. By contrast, microtubule-associated protein 2 is limited to the shafts of dendrites where time-lapse recordings show little evidence for dynamic activity. A parallel exists between the partitioning of microfilaments and microtubules in motile and stable domains of growing processes during development and between dendrite shafts and spines at excitatory synapses in established neuronal circuits. These data thus suggest a mechanism, conserved through development and adulthood, in which the differential dynamics of actin and microtubules determine the plasticity of neuronal structures.

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  • LMW MAP2C lacking PD still localizes to the soma and dendrites.

    “although LMW MAP2C lacking PD still localizes to the soma and dendrites (11).”

    From Active transport by cytoplasmic dynein sustains dendritic MAP2 localization in developing neurons (2026) · cites this paper as [11]

    The Results (page unavailable) report that MAP2c-GFP is detectable in dendrites and, more weakly, in cell bodies. They do not report localization of MAP2c lacking PD, so the deletion-specific part of the claim is unsupported. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • Expressed MAP2 without any native mRNA structures such as a 3′-untranslated region still localizes properly to dendrites.

    “However, we (this paper) and others have shown that expressed MAP2 without any native mRNA structures such as a 3′-untranslated region, which are typically necessary for mRNA transport and local translation (55), still localizes properly to dendrites (11).”

    From Active transport by cytoplasmic dynein sustains dendritic MAP2 localization in developing neurons (2026) · cites this paper as [11]

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Cited by

Sorted by citations

Authors
Kaech, S., Parmar, H., Roelandse, M., Bornmann, C., & Matus, A.
Affiliations
Friedrich Miescher Institute (CH)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2001-06-19
Volume
98
Issue
13
Pages
7086-7092
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.111146798
PMC
PMC34627
OpenAlex
W2170191832
Cited by
124
References
5
Citations by year
2026: 3 · 2025: 3 · 2024: 3 · 2023: 1 · 2022: 5 · 2021: 4 · 2020: 3 · 2019: 5 · 2018: 3 · 2017: 5 · 2016: 4 · 2015: 3 · 2013: 5 · 2012: 4
FWCI
2.96
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
Keywords
dendritic morphological plasticity, dendritic spines, F-actin, microtubules, MAP2, cytoskeletal dynamics, excitatory synapses, neuronal development, time-lapse imaging
MeSH terms
Actins, Animals, Cells, Cultured, Chickens, Cytoskeleton, Dendrites, Hippocampus, Luminescent Proteins, Mice, Transgenic, Microtubule-Associated Proteins, Neuronal Plasticity, Neurons, Transfection, Genes, Reporter, Green Fluorescent Proteins, Mice

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