Cited by 1,276Neuroscience and Neuropharmacology ResearchReceptor Mechanisms and SignalingGenetics and Neurodevelopmental Disorders

Rapid Spine Delivery and Redistribution of AMPA Receptors After Synaptic NMDA Receptor Activation

Shi, Song-Hai, Hayashi, Yasunori, Petralia, Ronald S., Zaman, Shahid H., Wenthold, Robert J., Svoboda, Karel, Malinow, Roberto·1999·Science

Abstract

To monitor changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptor distribution in living neurons, the AMPA receptor subunit GluR1 was tagged with green fluorescent protein (GFP). This protein (GluR1-GFP) was functional and was transiently expressed in hippocampal CA1 neurons. In dendrites visualized with two-photon laser scanning microscopy or electron microscopy, most of the GluR1-GFP was intracellular, mimicking endogenous GluR1 distribution. Tetanic synaptic stimulation induced a rapid delivery of tagged receptors into dendritic spines as well as clusters in dendrites. These postsynaptic trafficking events required synaptic N-methyl-D-aspartate (NMDA) receptor activation and may contribute to the enhanced AMPA receptor-mediatedtransmission observed during long-term potentiation and activity-dependent synaptic maturation.

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Crossref · 10.1126/science.284.5421.1811

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2 of 2 references verified0 citation errors · 0 not checkable

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Authors
Shi, S.-H., Hayashi, Y., Petralia, R. S., Zaman, S. H., Wenthold, R. J., Svoboda, K., & Malinow, R.
Affiliations
  • Cold Spring Harbor Laboratory (US)
  • National Institutes of Health (US)
Corresponding author
Malinow, Roberto
Venue
Science
Published
1999-06-11
Volume
284
Issue
5421
Pages
1811-1816
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2132971431
Cited by
1,276
References
2
Citations by year
2026: 9 · 2025: 16 · 2024: 21 · 2023: 19 · 2022: 19 · 2021: 37 · 2020: 18 · 2019: 22 · 2018: 32 · 2017: 30 · 2016: 27 · 2015: 38 · 2014: 46 · 2013: 47 · 2012: 56
FWCI
35.36
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • Cold Spring Harbor Laboratory Institutional Repository (Cold Spring Harbor Laboratory) (acceptedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
Keywords
AMPA receptor trafficking, GluR1, dendritic spines, NMDA receptor activation, synaptic plasticity, long-term potentiation, CA1 pyramidal neurons, receptor redistribution
MeSH terms
Animals, Cells, Cultured, Dendrites, Electric Stimulation, Hippocampus, Humans, Synaptic Transmission, Neuronal Plasticity, Neurons, Organ Culture Techniques, Receptor Aggregation, Recombinant Fusion Proteins, Synapses, Tetany, Receptors, N-Methyl-D-Aspartate, Long-Term Potentiation, Receptors, AMPA, Rats

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