High actin concentrations in brain dendritic spines and postsynaptic densities.
Open accessAbstract
Antibodies against actin were used to corroborate the presence of actin as a major component protein of isolated brain postsynaptic densities. The same antibodies also were used as an immunohistochemical stain to study the distribution of actin in sections of intact brain tissue. This showed two major sites where actin is concentrated: smooth muscle cells around blood vessels and postsynaptic sites. In the postsynaptic area the highest concentration of actin occurs in postsynaptic densities and there also is intense staining in the surrounding cytoplasm, especially within dendritic spines. Antiactin staining was much weaker in other parts of neurons and in glial cells. The high concentration of actin in dendritic spines may be related to shape changes that these structures have been found to undergo in response to prolonged afferent stimulation.
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Actin is highly enriched at spines compared with the surrounding neuropil.
“Immunoelectron microscopy studies demonstrated that actin is highly enriched at spines as compared with the surrounding neuropil (Matus et al., 1982;Cohen et al., 1985)”
From Regulation of F-Actin Stability in Dendritic Spines by Glutamate Receptors and Calcineurin (1998) · cites this paper as Matus et al., 1982
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“Immunoelectron microscopy studies demonstrated that actin is highly enriched at spines as compared with the surrounding neuropil ( Matus et al., 1982; Cohen et al., 1985), and direct observations of actin filaments identified by heavy meromyosin labeling confirm a much greater density of actin filaments in spines comp…”
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Self-citation
- Authors
- Matus, A., Ackermann, M., Pehling, G., Byers, H. R., & Fujiwara, K.
- ORCID
- Fujiwara, K
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1982-12
- Volume
- 79
- Issue
- 23
- Pages
- 7590-7594
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC347386
- OpenAlex
- W1966706797
- Cited by
- 401
- References
- 1
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 4 · 2023: 10 · 2022: 4 · 2021: 6 · 2020: 6 · 2019: 3 · 2018: 4 · 2017: 13 · 2016: 16 · 2015: 10 · 2014: 11 · 2013: 16 · 2012: 12
- FWCI
- 4.06
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- actin, dendritic spines, postsynaptic density, brain, smooth muscle cells, actin distribution, synaptic plasticity, glial cells, neurons
- MeSH terms
- Actins, Animals, Brain, Brain Chemistry, Dendrites, Immunoenzyme Techniques, Microscopy, Electron, Synapses, Rats
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