Converging on a mechanism for choline degradation

Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease

Wang, Z., Klipfell, E., Bennett, B. J., Koeth, R., Levison, B. S., DuGar, B., Feldstein, A. E., Britt, E. B., Fu, X., Chung, Y.-M., et al. (2011) · Nature · doi:10.1038/nature09922 · Cited by 5,597 · see citing works

Choline: an essential nutrient for public health

Zeisel, S. H., & da Costa, K.-A. (2009) · Nutrition Reviews · doi:10.1111/j.1753-4887.2009.00246.x · Cited by 1,062 · see citing works

Metabolic profiling reveals a contribution of gut microbiota to fatty liver phenotype in insulin-resistant mice

Dumas, M.-E., Barton, R. H., Toye, A., Cloarec, O., Blancher, C., Rothwell, A., Fearnside, J., Tatoud, R., Blanc, V., Lindon, J. C., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0601056103 · Cited by 1,042 · see citing works

Chemistry and biochemistry of B12

Banerjee, R. V. (1999) · Cited by 713 · see citing works

Microbial conversion of choline to trimethylamine requires a glycyl radical enzyme

Craciun, S., & Balskus, E. P. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1215689109 · Cited by 701 · see citing works

Ethanolamine utilization in bacterial pathogens: roles and regulation

Garsin, D. A. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2334 · Cited by 313 · see citing works

Is it time to reevaluate methyl balance in humans?

Stead, L. M., Brosnan, J. T., Brosnan, M. E., Vance, D. E., & Jacobs, R. L. (2006) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/83.1.5 · Cited by 278 · see citing works

Radical Mechanisms of Enzymatic Catalysis

Frey, P. A. (2001) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.70.1.121 · Cited by 218 · see citing works

Radical Enzymes in Anaerobes

Buckel, W., & Golding, B. T. (2006) · Annual Review of Microbiology · doi:10.1146/annurev.micro.60.080805.142216 · Cited by 193 · see citing works

Insight into the Mechanism of the B 12 -Independent Glycerol Dehydratase from Clostridium butyricum : Preliminary Biochemical and Structural Characterization

O'Brien, J. R., Raynaud, C., Croux, C., Girbal, L., Soucaille, P., & Lanzilotta, W. N. (2004) · Biochemistry · doi:10.1021/bi035930k · Cited by 152 · see citing works

New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteria

Selmer, T., Pierik, A. J., & Heider, J. (2005) · Biological Chemistry · doi:10.1515/bc.2005.114 · Cited by 138 · see citing works

The Clostridial Fermentations of Choline and Ethanolamine

Bradbeer, C. (1965) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)97007-9 · Cited by 116 · see citing works

Crystal Structures of Ethanolamine Ammonia-lyase Complexed with Coenzyme B12 Analogs and Substrates

Shibata, N., Tamagaki, H., Hieda, N., Akita, K., Komori, H., Shomura, Y., Terawaki, S., Mori, K., Yasuoka, N., Higuchi, Y., et al. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.125112 · Cited by 100 · see citing works

Modeling the Reactions Catalyzed by Coenzyme B12-Dependent Enzymes

Sandala, G. M., Smith, D. M., & Radom, L. (2010) · Accounts of Chemical Research · doi:10.1021/ar900260c · Cited by 64 · see citing works

Understanding the Mechanism of Action of B12-Dependent Ethanolamine Ammonia-Lyase: Synergistic Interactions at Play

Wetmore, S. D., Smith, D. M., Bennett, J., & Radom, L. (2002) · Journal of the American Chemical Society · doi:10.1021/ja027579g · Cited by 63 · see citing works

Identification of cis-Ethanesemidione as the Organic Radical Derived from Glycolaldehyde in the Suicide Inactivation of Dioldehydrase and of Ethanolamine Ammonia-Lyase

Abend, A. M., Bandarian, V., Reed, G. H., & Frey, P. A. (2000) · Biochemistry · doi:10.1021/bi992963k · Cited by 47 · see citing works

Computational Exploration of Rearrangements Related to the Vitamin B12-Dependent Ethanolamine Ammonia Lyase Catalyzed Transformation

Semialjac, M., & Schwarz, H. (2002) · Journal of the American Chemical Society · doi:10.1021/ja020101s · Cited by 41 · see citing works

Anaerobic Degradation of Choline

Hayward, H. R. (1960) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64514-4 · Cited by 40 · see citing works

Glycerol Dehydratation by the B12-Independent Enzyme May Not Involve the Migration of a Hydroxyl Group: A Computational Study

Feliks, M., & Ullmann, G. M. (2012) · The Journal of Physical Chemistry B · doi:10.1021/jp301165b · Cited by 39 · see citing works

Identification of the Substrate Radical Intermediate Derived from Ethanolamine during Catalysis by Ethanolamine Ammonia-Lyase

Bender, G., Poyner, R. R., & Reed, G. H. (2008) · Biochemistry · doi:10.1021/bi801316v · Cited by 35 · see citing works

Ethanolamine ammonia-lyase.

Bandarian, V., Reed, G. H., & Banerjee, R. (1999) · Cited by 23 · see citing works

An insight into gut microbiota and its functionalities

Adak, A., & Khan, M. R. (2019) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-018-2943-4 · Cited by 1,485 · see citing works

RadicalS-Adenosylmethionine Enzymes

Broderick, J. B., Duffus, B. R., Duschene, K. S., & Shepard, E. M. (2014) · Chemical Reviews · doi:10.1021/cr4004709 · Cited by 827 · see citing works

Intestinal Microbiota Composition Modulates Choline Bioavailability from Diet and Accumulation of the Proatherogenic Metabolite Trimethylamine- <i>N</i> -Oxide

Romano, K. A., Vivas, E. I., Amador-Noguez, D., & Rey, F. E. (2015) · mBio · doi:10.1128/mbio.02481-14 · Cited by 777 · see citing works

Trimethylamine <i>N</i> -Oxide, the Microbiome, and Heart and Kidney Disease

Zeisel, S. H., & Warrier, M. (2017) · Annual Review of Nutrition · doi:10.1146/annurev-nutr-071816-064732 · Cited by 475 · see citing works

Microbiology Meets Big Data: The Case of Gut Microbiota–Derived Trimethylamine

Falony, G., Vieira-Silva, S., & Raes, J. (2015) · Annual Review of Microbiology · doi:10.1146/annurev-micro-091014-104422 · Cited by 187 · see citing works

Integrated metagenomics identifies a crucial role for trimethylamine-producing Lachnoclostridium in promoting atherosclerosis

Cai, Y., Huang, F., Lao, X., Lu, Y., Gao, X. J., Alolga, R. N., Yin, K., Zhou, X., Wang, Y., Liu, B., et al. (2022) · npj Biofilms and Microbiomes · doi:10.1038/s41522-022-00273-4 · Cited by 178 · see citing works

Integration of microbiome and epigenome to decipher the pathogenesis of autoimmune diseases

Chen, B., Sun, L., & Zhang, X. (2017) · Journal of Autoimmunity · doi:10.1016/j.jaut.2017.03.009 · Cited by 167 · see citing works

The microbial gbu gene cluster links cardiovascular disease risk associated with red meat consumption to microbiota l-carnitine catabolism

Buffa, J. A., Romano, K. A., Copeland, M. F., Cody, D. B., Zhu, W., Galvez, R., Fu, X., Ward, K., Ferrell, M., Dai, H. J., et al. (2021) · Nature Microbiology · doi:10.1038/s41564-021-01010-x · Cited by 114 · see citing works

Glycyl radical activating enzymes: Structure, mechanism, and substrate interactions

Shisler, K. A., & Broderick, J. B. (2014) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2014.01.020 · Cited by 102 · see citing works

Structure and Function of CutC Choline Lyase from Human Microbiota Bacterium Klebsiella pneumoniae

Kalnins, G., Kuka, J., Grı̄nberga, S., Makrecka‐Kuka, M., Liepinsh, E., Dambrova, M., & Tārs, K. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.670471 · Cited by 101 · see citing works

Role of the Microbiome in Gut-Heart-Kidney Cross Talk

Glorieux, G., Nigám, S. K., Vanholder, R. C., & Verbeke, F. (2023) · Circulation Research · doi:10.1161/circresaha.123.321763 · Cited by 75 · see citing works

Clinical significance and potential role of trimethylamine N-oxide in neurological and neuropsychiatric disorders

Mudimela, S., Vishwanath, N. K., Pillai, A., Morales, R., Marrelli, S. P., Barichello, T., & Giridharan, V. V. (2022) · Drug Discovery Today · doi:10.1016/j.drudis.2022.08.002 · Cited by 70 · see citing works

The gene expression and bioinformatic analysis of choline trimethylamine-lyase (CutC) and its activating enzyme (CutD) for gut microbes and comparison with their TMA production levels

Ramireddy, L., Tsen, H., Chiang, Y.-C., Hung, C.-Y., Chen, F., & Yen, H. T. (2021) · Current Research in Microbial Sciences · doi:10.1016/j.crmicr.2021.100043 · Cited by 30 · see citing works

Dietary Methionine Restriction Alleviates Choline-Induced Tri-Methylamine-N-Oxide (TMAO) Elevation by Manipulating Gut Microbiota in Mice

Lu, M., Yang, Y., Xu, Y., Wang, X., Li, B., Le, G., & Xie, Y. (2023) · Nutrients · doi:10.3390/nu15010206 · Cited by 32 · see citing works

The Relationship Between Early-Life Environment, The Epigenome and the Microbiota

Majnik, A. V., & Lane, R. H. (2015) · Epigenomics · doi:10.2217/epi.15.74 · Cited by 28 · see citing works

Trimethylamine N-Oxide (TMAO) and cancer risk: Insights into a possible link

Saha, B., Banerjee, A., Pathak, R., Duttaroy, A. K., & Pathak, S. (2025) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2025.118592 · Cited by 24 · see citing works

Exploring the roles of intestinal flora in enhanced recovery after surgery

Liu, Z., Li, N., Dang, Q., Liu, L., Wang, L., Li, H., & Han, X. (2023) · iScience · doi:10.1016/j.isci.2023.105959 · Cited by 21 · see citing works

Helicobacter Pylori Infection Induces Intestinal Dysbiosis That Could Be Related to the Onset of Atherosclerosis

Avilés‐Jiménez, F. (2022) · BioMed Research International · doi:10.1155/2022/9943158 · Cited by 19 · see citing works

Gut-Microbiota-Driven Lipid Metabolism: Mechanisms and Applications in Swine Production

Xiong, S. (2025) · Metabolites · doi:10.3390/metabo15040248 · Cited by 16 · see citing works

Revealing the Mechanism of Isethionate Sulfite-Lyase by QM/MM Calculations

Deng, W., Lü, Y., & Liao, R. (2021) · Journal of Chemical Information and Modeling · doi:10.1021/acs.jcim.1c00978 · Cited by 7 · see citing works

Mechanistic Insights into Choline Degradation Catalyzed by the Choline Trimethylamine-Lyase CutC

Yang, Y., Deng, W., & Liao, R. (2025) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.5c01496 · Cited by 5 · see citing works

All Roads Lead to Carbinolamine: QM/MM Study of Enzymatic C–N Bond Cleavage in Anaerobic Glycyl Radical Enzyme Choline Trimethylamine-Lyase (CutC)

Hanževački, M., Güven, J. J., Hinchliffe, P., Shaw, J., Mey, A. S. J. S., Fey, N., SPENCER, J. M., & Mulholland, A. J. (2025) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.5c04023 · Cited by 5 · see citing works

Proteomic analysis of homocholine catabolic pathway in Pseudomonas sp. strain A9

Ahmed, I. A. M., Eltayeb, M. M., Mori, N., Arima, J., TANAKA, H. K. M., Taniguchi, T., & Yamanaka, N. (2015) · Process Biochemistry · doi:10.1016/j.procbio.2015.07.001 · Cited by 4 · see citing works

Gut microbiota and diet in colorectal cancer: Converging determinants of carcinogenesis

Thakur, B. K., Choudhury, S. R., Turpin, W., & Martín, A. (2026) · Gut Microbes · doi:10.1080/19490976.2026.2664684 · Cited by 4 · see citing works

Identification of the key genes involved in the degradation of homocholine by Pseudomonas sp. strain A9 by using suppression subtractive hybridization

Ahmed, I. A. M., Eltayeb, M. M., Habora, M. E. E., Eltayeb, A. E., Arima, J., Mori, N., Taniguchi, T., & Yamanaka, N. (2016) · Process Biochemistry · doi:10.1016/j.procbio.2016.10.009 · Cited by 2 · see citing works

Purification and characterization of 4-N-trimethylamino-1-butanol dehydrogenase from Fusarium merismoides var. acetilereum

Fujimitsu, H., Taniyama, Y., Tajima, S., Ahmed, I. A. M., Arima, J., & Mori, N. (2016) · Bioscience Biotechnology and Biochemistry · doi:10.1080/09168451.2016.1177443 · Cited by 1 · see citing works

Computational insights into chemoselectivity of Trans-4-Hydroxy-L-Proline dehydratase HypD

Deng, W., Lü, Y., & Liao, R. (2024) · Journal of Catalysis · doi:10.1016/j.jcat.2024.115736 · Cited by 1 · see citing works

Steric and Electronic Effects in the Enzymatic Catalysis of Choline-TMA Lyase

Gogonea, V., & Hazen, S. L. (2025) · Biomolecules · doi:10.3390/biom16010037

Microbial conversion of choline to trimethylamine requires a glycyl radical enzyme

Craciun, S., & Balskus, E. P. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1215689109 · Cited by 701 · see citing works

A cobalamin-dependent pathway of choline demethylation from the human gut acetogen Eubacterium limosum

Jiang, R., Kountz, D. J., Zhang, L., & Krzycki, J. A. (2025) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2025.108524 · Cited by 9 · see citing works

Isolation and Characterization of a Novel Choline Degrading Citrobacter amalonaticus Strain from the Human GutPreprint

Kashyap, J., Ferguson, D. J., & Schwab, N. R. (2021) · SSRN Electronic Journal · doi:10.2139/ssrn.3958741

Bacterial Degradation of Choline

EDDY, B. P. (1953) · Nature · doi:10.1038/171573b0 · Cited by 15 · see citing works

Radical Chemistry in the Human Gut: Discovery of Choline Trimethylamine‐Lyase

Bodea, S., & Balskus, E. P. (2015) · The FASEB Journal · doi:10.1096/fasebj.29.1_supplement.575.15