Posttraining optogenetic manipulations of basolateral amygdala activity modulate consolidation of inhibitory avoidance memory in rats
Open accessAbstract
Memory consolidation studies, including those examining the role of the basolateral amygdala (BLA), have traditionally used techniques limited in their temporal and spatial precision. The development of optogenetics provides increased precision in the control of neuronal activity that can be used to address the temporal nature of the modulation of memory consolidation. The present experiments, therefore, investigated whether optogenetically stimulating and inhibiting BLA activity immediately after training on an inhibitory avoidance task enhances and impairs retention, respectively. The BLA of male Sprague–Dawley rats was transduced to express either ChR2(E123A) or archaerhodopsin-3 from the Halorubrum sodomense strain TP009 (ArchT). Immediately after inhibitory avoidance training, rats received optical stimulation or inhibition of the BLA, and 2 d later, rats’ retention was tested. Stimulation of ChR2(E123A)-expressing neurons in the BLA using trains of 40-Hz light pulses enhanced retention, consistent with recording studies suggesting the importance of BLA activity at this frequency. Light pulses alone given to control rats had no effect on retention. Inhibition of ArchT-expressing neurons in the BLA for 15 min, but not 1 min, significantly impaired retention. Again, illumination alone given to control rats had no effect on retention, and BLA inhibition 3 h after training had no effect. These findings provide critical evidence of the importance of specific frequency patterns of activity in the BLA during consolidation and indicate that optogenetic manipulations can be used to alter activity after a learning event to investigate the processes underlying memory consolidation.
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Photoactivation of BLA somata increases measures of innate anxiety, as well as learned inhibitory avoidance.
“Moreover, photoactivation of BLA somata increases measures of innate anxiety33, as well as learned inhibitory avoidance80.”
From Resolving the neural circuits of anxiety (2015) · cites this paper as [80]
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- Authors
- Huff, M. L., Miller, R. L., Deisseroth, K., Moorman, D. E., & LaLumiere, R. T.
- Affiliations
- University of Iowa (US)
- Howard Hughes Medical Institute (US)
- Medical University of South Carolina (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2013-02-11
- Volume
- 110
- Issue
- 9
- Pages
- 3597-3602
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC3587230
- OpenAlex
- W2069088539
- Cited by
- 104
- References
- 41
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 8 · 2023: 4 · 2022: 7 · 2021: 7 · 2020: 6 · 2019: 8 · 2018: 4 · 2017: 12 · 2016: 8 · 2015: 11 · 2014: 13 · 2013: 5
- FWCI
- 6.74
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institute of Mental Health (R21MH097111, MH097111)
- National Institute on Drug Abuse (R21DA032005, DA032005)
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory Processes and Influences
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Keywords
- basolateral amygdala, memory consolidation, passive avoidance, optogenetics, archaerhodopsin-3, photostimulation, neuronal inhibition, memory retention
- MeSH terms
- Channelrhodopsins, Amygdala, Animals, Avoidance Learning, Male, Memory, Neurons, Retention, Psychology, Transduction, Genetic, Rats, Sprague-Dawley, Proton Pumps, Archaeal Proteins, Amino Acid Substitution, Rats, Optogenetics
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