Cited by 72Microbial Metabolic Engineering and BioproductionPlant biochemistry and biosynthesisAlgal biology and biofuel production

Genetically engineering Synechocystis sp. Pasteur Culture Collection 6803 for the sustainable production of the plant secondary metabolite p -coumaric acid

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Xue, Yong, Zhang, Yan, Cheng, Dan, Daddy, Soumana, He, Qingfang·2014·Proceedings of the National Academy of Sciences

Abstract

p-Coumaric acid is the precursor of phenylpropanoids, which are plant secondary metabolites that are beneficial to human health. Tyrosine ammonia lyase catalyzes the production of p-coumaric acid from tyrosine. Because of their photosynthetic ability and biosynthetic versatility, cyanobacteria are promising candidates for the production of certain plant metabolites, including phenylpropanoids. Here, we produced p-coumaric acid in a strain of transgenic cyanobacterium Synechocystis sp. Pasteur Culture Collection 6803 (hereafter Synechocystis 6803). Whereas a strain of Synechocystis 6803 genetically engineered to express sam8, a tyrosine ammonia lyase gene from the actinomycete Saccharothrix espanaensis, accumulated little or no p-coumaric acid, a strain that both expressed sam8 and lacked slr1573, a native hypothetical gene shown here to encode a laccase that oxidizes polyphenols, produced ∼82.6 mg/L p-coumaric acid, which was readily purified from the growth medium.

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Crossref · 10.1073/pnas.1323725111

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  • Xue et al. engineered Synechocystis sp. PCC 6803 by introducing the tyrosine ammonia lyase gene sam8 from Saccharothrix espanaensis and deleting the native slr1573 laccase-like gene, resulting in secretion of approximately 82.6 mg/L p-coumaric acid into the culture medium.

    “Xue et al. engineered Synechocystis sp. PCC 6803 by introducing the tyrosine ammonia lyase gene sam8 from Saccharothrix espanaensis and deleting the native slr1573 laccase-like gene, resulting in secretion of approximately 82.6 mg/L p-coumaric acid into the culture medium [185].”

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  1. Lee KH (1999) Novel antitumor agents from higher plants. Med Res Rev 19(6):569-596.

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Authors
Xue, Y., Zhang, Y., Cheng, D., Daddy, S., & He, Q.
Affiliations
  • University of Arkansas at Little Rock (US)
  • Shandong Academy of Agricultural Sciences (CN)
Venue
Proceedings of the National Academy of Sciences
Published
2014-06-09
Volume
111
Issue
26
Pages
9449-9454
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2088141494
Cited by
72
References
31
Citations by year
2026: 4 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 6 · 2021: 9 · 2020: 4 · 2019: 6 · 2018: 6 · 2017: 8 · 2016: 5 · 2015: 11 · 2014: 2
FWCI
3.55
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Science Foundation (1120153)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Keywords
p-coumaric acid, phenylpropanoids, Synechocystis sp. PCC 6803, tyrosine ammonia-lyase, laccase, transgenic cyanobacteria, plant secondary metabolites, polyphenol oxidation
MeSH terms
Ammonia-Lyases, Chromatography, High Pressure Liquid, Coumaric Acids, Genetic Engineering, Plasmids, Propionates, Mass Spectrometry, Molecular Structure, DNA Primers, Gene Transfer Techniques, Bioreactors, Reverse Transcriptase Polymerase Chain Reaction, Synechocystis, Biosynthetic Pathways

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