Potential of Producing Flavonoids Using Cyanobacteria as a Sustainable Chassis
Abstract
Numerous plant secondary metabolites have remarkable impacts on both food supplements and pharmaceuticals for human health improvement. However, higher plants can only generate small amounts of these chemicals with specific temporal and spatial arrangements, which are unable to satisfy the expanding market demands. Cyanobacteria can directly utilize CO 2, light energy, and inorganic nutrients to synthesize versatile plant-specific photosynthetic intermediates and organic compounds in large-scale photobioreactors with outstanding economic merit. Thus, they have been rapidly developed as a “green” chassis for the synthesis of bioproducts. Flavonoids, chemical compounds based on aromatic amino acids, are considered to be indispensable components in a variety of nutraceutical, pharmaceutical, and cosmetic applications. In contrast to heterotrophic metabolic engineering pioneers, such as yeast and Escherichia coli, information about the biosynthesis flavonoids and their derivatives is less comprehensive than that of their photosynthetic counterparts. Here, we review both benefits and challenges to promote cyanobacterial cell factories for flavonoid biosynthesis. With increasing concerns about global environmental issues and food security, we are confident that energy self-supporting cyanobacteria will attract increasing attention for the generation of different kinds of bioproducts. We hope that the work presented here will serve as an index and encourage more scientists to join in the relevant research area.
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Complex natural product biosynthesis in cyanobacteria faces bottlenecks including precursor competition, malonyl-CoA availability, pathway enzyme compatibility, and the need to balance heterologous pathway demand with photosynthetic ATP and NADPH supply.
“At the same time, they highlight important remaining bottlenecks for complex natural product biosynthesis in cyanobacteria, including precursor competition, malonyl-CoA availability, pathway enzyme compatibility and the need to balance heterologous pathway demand with photosynthetic ATP and NADPH supply [187].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [187]
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Engineering cyanobacteria for the production of aromatic natural products
Gu, F., Li, C., Zheng, H., & Ni, J. (2024) · Blue Biotechnology · doi:10.1186/s44315-024-00002-w · Cited by 9 · see citing works
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“…time, they highlight important remaining bottlenecks for complex natural product biosynthesis in cyanobacteria, including precursor competition, malonyl-CoA availability, pathway enzyme compatibility and the need to balance heterologous pathway demand with photosynthetic ATP and NADPH supply [187].”
- Authors
- Jin, H., Wang, Y., Zhao, P., Wang, L., Zhang, S., Meng, D., Yang, Q., Cheong, L.-Z., Bi, Y., & Fu, Y.
- Affiliations
- Beijing Forestry University (CN)
- Peking University Third Hospital (CN)
- College of Forestry, Beijing Forestry University, Beijing 100083, P.R. China
- Ningbo University (CN)
- Chinese Academy of Sciences (CN)
- Corresponding author
- Jin, Haojie
- Fu, Yujie
- ORCID
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2021-10-15
- Volume
- 69
- Issue
- 42
- Pages
- 12385-12401
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W3206930110
- Cited by
- 17
- References
- 212
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 5 · 2023: 5 · 2022: 4
- FWCI
- 0.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education of the People's Republic of China (2021ZY06)
- Ministry of Science and Technology of the People's Republic of China (2020YFA0907402)
- National Natural Science Foundation of China (31930076)
- Key Laboratory of Forest Plant Ecology, Ministry of Education Northeast Forestry University (K2020A01)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Keywords
- flavonoids, cyanobacteria, flavonoid biosynthesis, plant secondary metabolites, photobioreactors, bioproducts, nutraceuticals, sustainable biomanufacturing, photosynthetic intermediates
- MeSH terms
- Cyanobacteria, Flavonoids, Humans, Photosynthesis, Amino Acids, Aromatic, Metabolic Engineering
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