Cited by 55Genomics and Chromatin DynamicsRNA and protein synthesis mechanismsNuclear Structure and Function

Transcription factors IIS and IIF enhance transcription efficiency by differentially modifying RNA polymerase pausing dynamics

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Ishibashi, Toyotaka, Dangkulwanich, Manchuta, Coello, Yves, Lionberger, Troy A., Lubkowska, Lucyna, Ponticelli, Alfred S., Kashlev, Mikhail, Bustamante, Carlos·2014·Proceedings of the National Academy of Sciences

Abstract

Significance: Regulation of gene expression controls fundamental cellular processes, such as growth and differentiation. Gene expression begins at transcription, in which the eukaryotic RNA polymerase (Pol II) synthesizes the precursors of mRNA during the elongation phase. The speed and fidelity of the process are regulated by many transcription elongation factors, including transcription factors IIS (TFIIS) and IIF (TFIIF), which directly interact with the enzyme. Using a single-molecule optical-tweezers assay, we have quantified the effects of these factors on the dynamics of transcription elongation by Pol II on both bare and nucleosomal DNA. We showed that TFIIF mainly prevents Pol II from pause entering, whereas TFIIS assists with pause recovery, and quantitatively described these effects on the kinetics of transcription elongation by Pol II.

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Crossref · 10.1073/pnas.1401611111

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Authors
Ishibashi, T., Dangkulwanich, M., Coello, Y., Lionberger, T. A., Lubkowska, L., Ponticelli, A. S., Kashlev, M., & Bustamante, C.
Affiliations
  • QB3 (US)
  • Frederick National Laboratory for Cancer Research (US)
  • University at Buffalo, State University of New York (US)
Venue
Proceedings of the National Academy of Sciences
Published
2014-02-18
Volume
111
Issue
9
Pages
3419-3424
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2044001407
Cited by
55
References
40
Citations by year
2026: 1 · 2025: 3 · 2024: 1 · 2023: 4 · 2022: 3 · 2021: 3 · 2020: 5 · 2019: 3 · 2018: 5 · 2017: 9 · 2016: 13 · 2015: 2 · 2014: 3
FWCI
3.69
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institute of General Medical Sciences (R01GM032543)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
TFIIS, TFIIF, RNA polymerase II, transcription elongation, transcriptional pausing, polymerase processivity, transcription factors
MeSH terms
Escherichia coli, Gene Expression Regulation, Kinetics, Monte Carlo Method, DNA-Directed RNA Polymerases, RNA, Messenger, Saccharomyces cerevisiae, Transcription Factors, TFII, Transcriptional Elongation Factors, Optical Tweezers, Transcription Elongation, Genetic

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