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Transgenerational epigenetic programming via sperm microRNA recapitulates effects of paternal stress
Open accessAbstract
Significance: Studies examining paternal exposure to diverse environmental stimuli propose that epigenetic marks in germ cells, including small noncoding RNAs such as microRNA (miR), transmit experience-dependent information from parent to offspring. However, these nongenetic mechanisms of transgenerational inheritance are poorly understood, specifically how these germ-cell marks may act postfertilization to enact long-term changes in offspring behavior or physiology. In this study, through zygote microinjection of nine specific sperm miRs previously identified in our paternal stress mouse model, we demonstrate that sperm miRs function to reduce maternal mRNA stores in early zygotes, ultimately reprogramming gene expression in the offspring hypothalamus and recapitulating the offspring stress dysregulation phenotype.
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“This has almost exclusively been done in animal models, 63 , 64 , 65 , 66 with a few exceptions of studies in human cohorts. 67”
From Epigenetics and depression (2019) · cites this paper as [64]
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“Nonetheless, a few studies have shown environmentally driven changes of the epigenetic landscape in other ways, such as via histone modifications and long noncoding RNAs.”
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“and a mechanistic role for sperm microRNAs in the transgenerational transmission of stress reactivity to the offspring has also been demonstrated [70].”
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“They provide some compelling evidence for the transgenerational transmission and inheritance of environmentally acquired risk factors across generations via the germline [69], with epigenetic mechanisms strongly implicated [70].”
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Self-citation
Metadata
- Authors
- Rodgers, A. B., Morgan, C. P., Leu, N. A., & Bale, T. L.
- Affiliations
- University of Pennsylvania (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2015-10-19
- Volume
- 112
- Issue
- 44
- Pages
- 13699-13704
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC4640733
- OpenAlex
- W1949928978
- Cited by
- 751
- References
- 48
- Citations by year
- 2026: 50 · 2025: 40 · 2024: 54 · 2023: 74 · 2022: 69 · 2021: 70 · 2020: 81 · 2019: 67 · 2018: 107 · 2017: 72 · 2016: 61 · 2015: 3
- FWCI
- 35.19
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- HHS | NIH | National Institute of Mental Health (MH073030, MH091258, MH087597, MH099910, MH104184)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Birth, Development, and Health
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Keywords
- sperm microRNA, paternal stress, chronic stress, hypothalamic-pituitary-adrenal axis, zygote microinjection, blood-brain barrier permeability, extracellular matrix, collagen, maternal mRNA degradation, SIRT1, chromatin remodeling, germ cells, single-cell transcriptomics
- MeSH terms
- Animals, Hypothalamo-Hypophyseal System, Male, Mice, Inbred C57BL, Spermatozoa, Stress, Physiological, Paternal Exposure, MicroRNAs, Epigenesis, Genetic, Mice, Transcriptome
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