The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes
Open accessAbstract
Significance: The last decade has seen a rapid expansion of interest in extracellular vesicles (EVs), proposed to mediate cell–cell communication in patho/physiological conditions. Although heterogeneity of EVs has become obvious, as highlighted recently by the International Society for Extracellular Vesicles, the field is lacking specific tools to distinguish EVs of different intracellular origins, and thus probably different functions. Here, thanks to a comprehensive comparison of different types of EVs isolated from a single cell type, we define proteins generically present in EVs, small EV-specific and -excluded ones, and a few specific of endosome-derived exosomes or nonexosomal small EVs. This work will allow proper evaluation of the molecular mechanisms of biogenesis and secretion and the respective functions of subtypes of EVs.
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“The results imply that CD81-containing EVs, multivesicular bodies, and CD9-positive small EVs derived from the plasma membrane could accumulate in the DNs [39, 44].”
From Abnormal accumulation of extracellular vesicles in hippocampal dystrophic axons and regulation by the primary cilia in Alzheimer's disease (2023) · cites this paper as [39]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
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“Most mitochondrial components have been reported to fractionate in larger EVs by proteomic analysis [395].”
From Mechanisms, efficacy, and future perspectives of cellular-based therapies for liver fibrosis/cirrhosis: focusing on mesenchymal stromal cells (2025) · cites this paper as [395]
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“Furthermore, molecules encapsulated within blood-circulating EVs may also serve as diagnostic, prognostic, and predictive biomarkers.222,223”
From MiR-128-3p – a gray eminence of the human central nervous system (2024) · cites this paper as [222]
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“Heterogeneity of these different fractions was confirmed by probing for different vesicular protein markers that are present in all types of EVs and larger vesicle fractions (Flotillin-1) (Kowal et al., 2016), those typically associated with exosomes and absent in 2,000 × g (ALIX and TSG101), and GAPDH found to be enriched in the 2,000 × g and cellular fraction (McNamara et al., 2018).”
From Glioblastoma-Associated Microglia Reprogramming Is Mediated by Functional Transfer of Extracellular miR-21 (2019) · cites this paper as Kowal et al., 2016
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“EC-Exo co-localize with CD9 and CD63 which are exosome markers21, thereby verifying the fluorescence as clusters of exosomes.”
From MiR-126 Mediates Brain Endothelial Cell Exosome Treatment–Induced Neurorestorative Effects After Stroke in Type 2 Diabetes Mellitus Mice (2019) · cites this paper as [21]
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Citations
Cites
Extracellular vesicles: Exosomes, microvesicles, and friends
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Colombo, M., Raposo, G., & Théry, C. (2014) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-101512-122326 · Cited by 6,186
Self-citationBiological properties of extracellular vesicles and their physiological functions
Yáñez‐Mó, M., Siljander, P. R. ‐., Andreu, Z., Bedina Zavec, A., Borràs, F. E., Buzas, E. I., Buzas, K., Casal, E., Cappello, F., Carvalho, J., et al. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27066 · Cited by 5,926
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Théry, C., Amigorena, S., Raposo, G., & Clayton, A. (2006) · Current Protocols in Cell Biology · doi:10.1002/0471143030.cb0322s30 · Cited by 5,766
Self-citationYang, Y., Dudoit, S., Luu, P., Lin, D., Peng, V., Ngai, J., & Speed, T. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.4.e15 · Cited by 3,722
B lymphocytes secrete antigen-presenting vesicles.
Raposo, G., Nijman, H. W., Stoorvogel, W., Liejendekker, R., Harding, C. V., Melief, C. J., & Geuze, H. J. (1996) · The Journal of experimental medicine · doi:10.1084/jem.183.3.1161 · Cited by 3,546
Glypican-1 identifies cancer exosomes and detects early pancreatic cancer
Melo, S. A., Luecke, L. B., Kahlert, C., Fernandez, A. F., Gammon, S. T., Kaye, J., LeBleu, V. S., Mittendorf, E. A., Weitz, J., Rahbari, N., et al. (2015) · Nature · doi:10.1038/nature14581 · Cited by 2,880
Rab27a and Rab27b control different steps of the exosome secretion pathway
Ostrowski, M., Carmo, N., Krumeich, S., Fanget, I., Raposo, G., Savina, A., Moita, C. F., Schauer, K., Hume, A. N., Freitas, R. P., et al. (2009) · Nature Cell Biology · doi:10.1038/ncb2000 · Cited by 2,761
Self-citationRegulation of immune responses by extracellular vesicles
Robbins, P. D., & Morelli, A. E. (2014) · Nature Reviews Immunology · doi:10.1038/nri3622 · Cited by 2,260
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Khanal, S., Liu, Y., Bamidele, A. O., Wixom, A. Q., Washington, A. M., Jalan-Sakrikar, N., Cooper, S. A., Vuckovic, I., Zhang, S., Zhong, J., et al. (2024) · Science Advances · doi:10.1126/sciadv.adn5228 · Cited by 41
Raghavan, K. S., Francescone, R., Franco-Barraza, J., Gardiner, J. C., Vendramini-Costa, D. B., Luong, T., Pourmandi, N., Andren, A., Kurimchak, A., Ogier, C., et al. (2022) · Cancer Research Communications · doi:10.1158/2767-9764.crc-21-0147 · Cited by 40
Jang, J., Yeo, S., Baek, S., Jung, H. J., Lee, M. S., Choi, S. H., & Choe, Y. (2023) · Acta Neuropathologica Communications · doi:10.1186/s40478-023-01637-3 · Cited by 20
“The results imply that CD81-containing EVs, multivesicular bodies, and CD9-positive small EVs derived from the plasma membrane could accumulate in the DNs [39, 44].”
BackgroundRecent advances in our understanding of the allograft response
Hennessy, C., Lewik, G., Cross, A., Hester, J., & Issa, F. (2021) · Faculty Reviews · doi:10.12703/r/10-21 · Cited by 18
MiR-128-3p – a gray eminence of the human central nervous system
Kiel, K., Król, S. K., Bronisz, A., & Godlewski, J. (2024) · Molecular Therapy Nucleic Acids · doi:10.1016/j.omtn.2024.102141 · Cited by 15
Pan, X., Gao, T., & Wang, B. (2025) · Cell & Bioscience · doi:10.1186/s13578-025-01520-9 · Cited by 2
“Most mitochondrial components have been reported to fractionate in larger EVs by proteomic analysis [395].”
Metadata
- Authors
- Kowal, J., Arras, G., Colombo, M., Jouve, M., Morath, J. P., Primdal-Bengtson, B., Dingli, F., Loew, D., Tkach, M., & Théry, C.
- Affiliations
- Inserm (FR)
- Université Paris Sciences et Lettres (FR)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2016-02-08
- Volume
- 113
- Issue
- 8
- Pages
- E968-77
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC4776515
- OpenAlex
- W2259481044
- Cited by
- 3,426
- References
- 55
- Citations by year
- 2026: 247 · 2025: 296 · 2024: 336 · 2023: 322 · 2022: 455 · 2021: 454 · 2020: 444 · 2019: 312 · 2018: 278 · 2017: 213 · 2016: 63
- FWCI
- 150.25
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Funders
- Agence Nationale de la Recherche (ANR-10-IDEX-0001-02 PSL*, ANR-11-LABX-0043)
- Association pour la Recherche sur le Cancer (SL220120605293)
- Fondation pour la Recherche Médicale (DGE 20121125630)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
- Keywords
- extracellular vesicles, exosomes, dendritic cells, exosomal biomarkers, CD63, CD81, CD9, proteomic profiling, vesicle heterogeneity
- MeSH terms
- Humans, Membrane Proteins, Biomarkers, Antigens, CD, HSP70 Heat-Shock Proteins, Proteomics, Cell-Derived Microparticles
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