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Glutamate-induced transient modification of the postsynaptic density
Open accessAbstract
Depolarization of rat hippocampal neurons with a high concentration of external potassium induces a thickening of postsynaptic densities (PSDs) within 1.5-3 min. After high-potassium treatment, PSDs thicken 2.1-fold in cultured neurons and 1.4-fold in hippocampal slices compared with their respective controls. Thin-section immunoelectron microscopy of hippocampal cultures indicates that at least part of the observed thickening of PSDs can be accounted for by an accumulation of Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) on their cytoplasmic faces. Indeed, PSD-associated gold label for CaMKII increases 5-fold after depolarization with potassium. The effects of high-potassium treatment on the composition and structure of the PSDs are mimicked by direct application of glutamate. In cultures, glutamate-induced thickening of PSDs and the accumulation of CaMKII on PSDs are reversed within 5 min of removal of glutamate and Ca(2+) from the extracellular medium. These results suggest that PSDs are dynamic structures whose thickness and composition are subject to rapid and transient changes during synaptic activity.
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“activation of CaMKII results in the rapid translocation of CaMKII to the PSD (137, 140, 169, 216–220).”
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“Glutamate treatment of neuronal cultures causes CaMKII clustering at excitatory synapses (136–140).”
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“Indeed, electron microscopic studies show that raising Ca 2+ causes a build-up of CaMKII in the PS”
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Structure–function of the multifunctional Ca2+/calmodulin-dependent protein kinase II
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Wenthold, R. J., Prybylowski, K., Standley, S., Sans, N., & Petralia, R. S. (2003) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.43.100901.135803 · Cited by 374
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Jordan, B. A., Fernholz, B. D., Boussac, M., Xu, C., Grigorean, G., Ziff, E. B., & Neubert, T. A. (2004) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m400045-mcp200 · Cited by 296
“changes in synaptic strength (7, 8), including alterations that”
BackgroundOrganization of the core structure of the postsynaptic density
Chen, X., Winters, C. A., Azzam, R. N., Li, X., Galbraith, J. A., Leapman, R. D., & Reese, T. S. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0800897105 · Cited by 299
“4-nm Nanogold (47), and silver enhanced (HQ kit; Nanoprobes).”
BackgroundInfluentialSelf-citationThiagarajan, T. C., Piedras-Renterı́a, E. S., & Tsien, R. W. (2002) · Neuron · doi:10.1016/s0896-6273(02)01049-8 · Cited by 292
Zuber, B., Nikonenko, I., Klauser, P., Müller, D., & Dubochet, J. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0509527102 · Cited by 263
Otmakhov, N., Tao-Cheng, J.-H., Carpenter, S., Asrican, B., Dosemeci, A., Reese, T. S., & Lisman, J. (2004) · The Journal of Neuroscience · doi:10.1523/jneurosci.2350-04.2004 · Cited by 258
“There thus appear to be two forms of translocation, a transient form (Shen and Meyer, 1999; Shen et al., 2000; Dosemeci et al., 2001; Gleason et al., 2003) that may occur under some experimental conditions and a persistent form that occurs under conditions that induce LTP.”
BackgroundSelf-citationRole of the CaMKII/NMDA Receptor Complex in the Maintenance of Synaptic Strength
Sanhueza, M., Fernandez-Villalobos, G., Stein, I. S., Kasumova, G., Zhang, P., Bayer, K. U., Otmakhov, N., Hell, J. W., & Lisman, J. (2011) · Journal of Neuroscience · doi:10.1523/jneurosci.1250-11.2011 · Cited by 248
“This is consistent with the hypothesis that CN reduces the CaMKII/NMDAR complex in the PSD.”
BackgroundInfluentialTargeting of calcium/calmodulin-dependent protein kinase II
Colbran, R. J. (2004) · Biochemical Journal · doi:10.1042/bj20031547 · Cited by 241
Long-term potentiation: outstanding questions and attempted synthesis
Lisman, J. (2003) · Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences · doi:10.1098/rstb.2002.1242 · Cited by 240
“During synaptic activity, the kinase translocates from the cytoplasm to the PSD (Shen et al. 2000; Dosemeci et al. 2001).”
BackgroundOntogeny of postsynaptic density proteins at glutamatergic synapses
Petralia, R. S., Sans, N., Wang, Y.-X., & Wenthold, R. J. (2005) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2005.03.013 · Cited by 236
“Also, for CaMKII, high labeling subjacent to the PSD was shown in cultured hippocampal synapses; again, as in the previous example, this contrasted with labeling for PSD-95, which was restricted to a “compact region on the cleft side of the PSD” (Dosemeci et al., 2001).”
ResultMass of the postsynaptic density and enumeration of three key molecules
Chen, X., Vinade, L., Leapman, R. D., Petersen, J. D., Nakagawa, T., Phillips, T. M., Sheng, M., & Reese, T. S. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0505359102 · Cited by 236
Self-citationPetersen, J. D., Chen, X., Vinadé, L., Döṣemeci, A., Lisman, J. E., & Reese, T. S. (2003) · Journal of Neuroscience · doi:10.1523/jneurosci.23-35-11270.2003 · Cited by 212
“activity, CaMKII translocates to the PSD (Shen and Meyer, 1999; Dosemeci et al., 2001), where it can optimally detect Ca 2 entry”
Self-citationTao, C.-L., Liu, Y.-T., Sun, R., Zhang, B., Qi, L., Shivakoti, S., Tian, C.-L., Zhang, P., Lau, P.-M., Zhou, Z. H., et al. (2018) · The Journal of Neuroscience · doi:10.1523/jneurosci.1548-17.2017 · Cited by 207
“, 2016), and suggests the existence of multiple structural configurations, perhaps reflecting different states of their activation (Dosemeci et al., 2001) and plasticity (Bi and Poo, 1998; Montgomery and Madison, 2004).”
BackgroundMolecular anatomy of the postsynaptic density.
Okabe, S. (2007) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2007.01.006 · Cited by 225
Twenty Years of SynGAP Research: From Synapses to Cognition
Gamache, T. R., Araki, Y., & Huganir, R. L. (2020) · The Journal of Neuroscience · doi:10.1523/jneurosci.0420-19.2020 · Cited by 161
“The PSD thickens in response to robust depolarization of cultured neurons and neurons in brain slices, and does so on exquisitely short timescales (Dosemeci et al., 2001; Meyer et al., 2014).”
BackgroundComposition of the Synaptic PSD-95 Complex
Döṣemeci, A., Makusky, A. J., Jankowska‐Stephens, E., Yang, X., Slotta, D. J., & Markey, S. P. (2007) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m700040-mcp200 · Cited by 172
“Previous immuno-EM studies have shown that excitatory and ischemia-like conditions cause severalfold increases in CaMKII labeling of the PSD (19, 45, 46).”
BackgroundHudmon, A., LeBel, E., Roy, H., Sik, A., Schulman, H., Waxham, M. N., & De Koninck, P. (2005) · The Journal of Neuroscience · doi:10.1523/jneurosci.4698-04.2005 · Cited by 159
“For the assessment of postsynaptic translocation of GFP-CaMKII in neurons, independent cultures were fixed before and immediately after a 1 or 5 min glutamate/glycine treatment and then immunostained for PSD95.”
BackgroundInfluentialSharma, K., Fong, D. K., & Craig, A. M. (2006) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2006.01.010 · Cited by 150
Activity-driven postsynaptic translocation of CaMKII
Merrill, M. A., Chen, Y., Strack, S., & Hell, J. W. (2005) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2005.10.003 · Cited by 155
Nicoll, R. A., & Schulman, H. (2023) · Physiological Reviews · doi:10.1152/physrev.00034.2022 · Cited by 126
“Subsequent studies showed that activation of CaMKII results in the rapid translocation of CaMKII to the PSD (137, 140, 169, 216 – 220).”
BackgroundThe Postsynaptic Density: There Is More than Meets the Eye
Dosemeci, A., Weinberg, R. J., Reese, T. S., & Tao-Cheng, J.-H. (2016) · Frontiers in Synaptic Neuroscience · doi:10.3389/fnsyn.2016.00023 · Cited by 138
“The Pallium Becomes More ElectronDense as CaMKII Accumulates Thin-section EM of cultured hippocampal neurons reveals increased electron density at the pallium under excitatory conditions (Figure 3: top panels), a phenomenon we have described as PSD ‘‘thickening’’ (Dosemeci et al., 2001).”
BackgroundInfluentialSelf-citationTao-Cheng, J.-H., Gallant, P. E., Brightman, M., Döṣemeci, A., & Reese, T. S. (2007) · The Journal of Comparative Neurology · doi:10.1002/cne.21276 · Cited by 116
“This dispersed pattern was similar to that seen in somas and spines from dissociated hippocampal cultures under unstimulated control conditions (Tao-Cheng et al., 2001; Dosemeci et al., 2001).”
BackgroundMethodResultSelf-citationActivity-related redistribution of presynaptic proteins at the active zone
Tao-Cheng, J.-H. (2006) · Neuroscience · doi:10.1016/j.neuroscience.2006.04.061 · Cited by 105
Self-citationKohl, Z., Abdallah, N. B. B., Vogelgsang, J., Tischer, L., Deusser, J., Amato, D., Anderson, S., Müller, C., Riess, O., Masliah, E., et al. (2015) · Neurobiology of Disease · doi:10.1016/j.nbd.2015.10.021 · Cited by 89
Regulation of Signal Transduction by Protein Targeting: The Case for CaMKII
Bayer, K. U., & Schulman, H. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.6063 · Cited by 104
Alternative splicing modulates the frequency‐dependent response of CaMKII to Ca2+ oscillations
Bayer, K. U., Koninck, P. D., & Schulman, H. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf360 · Cited by 112
Excitotoxic neuroprotection and vulnerability with CaMKII inhibition
Ashpole, N. M., & Hudmon, A. (2011) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2011.02.003 · Cited by 99
“, 1990), and 3) ischemia induces CaMKII translocation (Aronowski et al., 1992; Morioka et al., 1995; Onodera et al., 1995 Aronowski and Grotta, 1996; Hu et al., 1998; Dosemeci et al., 2001) and phosphorylation of key post-synaptic substrates (i.”
Activity-Dependent Movements of Postsynaptic Scaffolds at Inhibitory Synapses
Hanus, C., Ehrensperger, M.-V., & Triller, A. (2006) · Journal of Neuroscience · doi:10.1523/jneurosci.5123-05.2006 · Cited by 105
Posttranslational Modifications Regulate the Postsynaptic Localization of PSD-95
Vallejo, D., Codocedo, J. F., & Inestrosa, N. C. (2016) · Molecular Neurobiology · doi:10.1007/s12035-016-9745-1 · Cited by 97
Li, D., Gao, X., Ma, X., Wang, M., Cheng, C., Xue, T., Gao, F., Shen, Y., Zhang, J., & Liu, Q. (2024) · Cell Metabolism · doi:10.1016/j.cmet.2024.02.011 · Cited by 93
CaMKIIα-driven, phosphatase-checked postsynaptic plasticity via phase separation
Cai, Q., Zeng, M., Wu, X., Wu, H., Zhan, Y., Tian, R., & Zhang, M. (2021) · Cell Research · doi:10.1038/s41422-020-00439-9 · Cited by 83
Myosin IXa Binds AMPAR and Regulates Synaptic Structure, LTP, and Cognitive Function
Folci, A., Murru, L., Vezzoli, E., Ponzoni, L., Gerosa, L., Moretto, E., Longo, F., Zapata, J., Braida, D., Pistillo, F., et al. (2016) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2016.00001 · Cited by 83
“The average thickness of the PSD was calculated as described in Dosemeci et al. (2001).”
MethodWhiting, B. A., Moiseff, A., & Rubio, M. E. (2009) · Neuroscience · doi:10.1016/j.neuroscience.2009.07.049 · Cited by 78
“The same imaging software was used to calculate the thickness of the PSD as described elsewhere (Dosemici et al., 2001;Rubio, 2006).”
MethodSessoms-Sikes, S., Honse, Y., Lovinger, D. M., & Colbran, R. J. (2005) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2005.01.006 · Cited by 76
Paredes, C. C., Antunes, F. M., & Rubio, M. E. (2016) · Journal of Neuroscience · doi:10.1523/jneurosci.0226-16.2016 · Cited by 72
“4) was analyzed as described previously by Dosemeci et al. (2001) and Rubio (2006) using ImageJ software (rsbweb.nih.gov/ij/; see Fig.”
MethodPreparation of postsynaptic density fraction from hippocampal slices and proteomic analysis
Döṣemeci, A., Tao-Cheng, J.-H., Vinadé, L., & Jaffe, H. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.11.069 · Cited by 72
Self-citationSchulman, H. (2004) · Journal of Neuroscience · doi:10.1523/jneurosci.3606-04.2004 · Cited by 71
“Thin section electron microscopy reveals a fivefold increase in CaMKII associated with the cytoplasmic face of PSDs after depolarization of hippocampal cultures that may primarily account for a doubling of PSD density (Dosemeci et al., 2001).”
BackgroundIschemia-induced modifications in hippocampal CA1 stratum radiatum excitatory synapses
Kovalenko, T., Osadchenko, I. O., Nikonenko, A. G., Lushnikova, I., Voronin, K. V., Nikonenko, I., Müller, D., & Skibo, G. G. (2006) · Hippocampus · doi:10.1002/hipo.20211 · Cited by 70
“In vitro studies in acute hippocampal slices from adult rats confirmed an implication of glutamate release and calcium influx, the key events of ischemic damage, in the mechanisms of PSD thickening (Dosemeci et al., 2001).”
CaMKII, an Enzyme on the Move: Regulation of Temporospatial Localization
Griffith, L. C. (2003) · Molecular Interventions · doi:10.1124/mi.3.7.386 · Cited by 68
Transient ultrasound stimulation has lasting effects on neuronal excitability
Clennell, B., Steward, T. G. J., Elley, M., Shin, E., Weston, M., Drinkwater, B. W., & Whitcomb, D. J. (2021) · Brain stimulation · doi:10.1016/j.brs.2021.01.003 · Cited by 67
“PSD thickness was calculated by dividing the area of the PSD including the electron dense scaffold by the length of the postsynaptic membrane as previously described [35].”
MethodActivity induced changes in the distribution of Shanks at hippocampal synapses
Tao-Cheng, J. H., Dosemeci, A., Gallant, P. E., Smith, C., & Reese, T. (2010) · Neuroscience · doi:10.1016/j.neuroscience.2010.03.041 · Cited by 61
“N IH -PA Author M anuscript N IH -PA Author M anuscript N IH -PA Author M anuscript 37°C. Incubation media (normal, high K+ and Ca2+-free in HEPES-buffered Krebs Ringer) were as described by (Dosemeci et al., 2001).”
InfluentialSelf-citationBarrio-Alonso, E., Hernández‐Vivanco, A., Walton, C. C., Perea, G., & Frade, J. M. (2018) · Scientific Reports · doi:10.1038/s41598-018-32708-4 · Cited by 67
Trafficking of AMPA Receptors at Plasma Membranes of Hippocampal Neurons
Tao-Cheng, J.-H., Crocker, V. T., Winters, C. A., Azzam, R., Chludzinski, J., & Reese, T. S. (2011) · The Journal of Neuroscience · doi:10.1523/jneurosci.4745-10.2011 · Cited by 82
“Depolarization in high K produced the most consistent synaptic response (Dosemeci et al., 2001; Tao-Cheng, 2006) among three activation protocols.”
BackgroundMethodSelf-citationAraki, S., Osuka, K., Takata, T., Tsuchiya, Y., & Watanabe, Y. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21217997 · Cited by 62
Tao-Cheng, J.-H., Thein, S., Yang, Y., Reese, T. S., & Gallant, P. E. (2014) · Neuroscience · doi:10.1016/j.neuroscience.2014.01.066 · Cited by 84
“, 2007), changes similar to those produced by high K depolarization (Dosemeci et al., 2001) and NMDA stimulation (Dosemeci et al.”
InfluentialSelf-citationSynGAP moves out of the core of the postsynaptic density upon depolarization
Yang, Y., Tao-Cheng, J.-H., Reese, T. S., & Döṣemeci, A. (2011) · Neuroscience · doi:10.1016/j.neuroscience.2011.06.061 · Cited by 58
“CaMKII, SynGAP and Ras have been proposed to be elements of a cascade involved in the reorganization of the actin cytoskeleton (Carisle et al., 2008).”
InfluentialSelf-citationInterplay of enzymatic and structural functions of Ca MKII in long‐term potentiation
Kim, K., Saneyoshi, T., Hosokawa, T., Okamoto, K., & Hayashi, Y. (2016) · Journal of Neurochemistry · doi:10.1111/jnc.13672 · Cited by 53
“CaMKII translocates from the dendritic shaft to spines and the PSD in an activity-dependent manner (Shen and Meyer 1999; Dosemeci et al. 2001; Feng et al. 2011; Bosch et al. 2014).”
BackgroundRubio, M. E. (2006) · Hearing Research · doi:10.1016/j.heares.2006.03.007 · Cited by 52
Identification of novel phosphorylation sites on postsynaptic density proteins
Jaffe, H., Vinadé, L., & Döṣemeci, A. (2004) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2004.06.122 · Cited by 48
Torres-Peraza, J. F., Giralt, A., García‐Martínez, J. M., Pedrosa, E., Canals, J. M., & Alberch, J. (2007) · Neurobiology of Disease · doi:10.1016/j.nbd.2007.10.003 · Cited by 46
“Following glutamate treatment, αCaMKII translocates to the postsynaptic density (PSD) (Dosemeci et al., 2001) where it phosphorylates NMDAR (Gardoni et al., 1998) and inhibits the pro-survival RAS/ERK pathway (Oh et al., 2004).”
BackgroundInfluentialSubcellular organization of camkii in rat hippocampal pyramidal neurons
Ding, J., Kennedy, M. B., & Weinberg, R. J. (2013) · Journal of Comparative Neurology · doi:10.1002/cne.23372 · Cited by 45
“Sustained elevation of calcium induces Ca21/calmodulin-dependent protein kinase II clusters in hippocampal neurons.”
MethodInfluentialInhibition of phosphatase activity prolongs NMDA-induced modification of the postsynaptic density
Döṣemeci, A., Vinadé, L., Winters, C. A., Reese, T. S., & Tao-Cheng, J.-H. (2002) · Journal of Neurocytology · doi:10.1023/a:1025735410738 · Cited by 44
Self-citationRapid turnover of spinules at synaptic terminals
Tao-Cheng, J.-H., Döṣemeci, A., Gallant, P. E., Miller, S., Galbraith, J. A., Winters, C. A., Azzam, R. N., & Reese, T. S. (2009) · Neuroscience · doi:10.1016/j.neuroscience.2009.02.031 · Cited by 43
“We have previously reported two types of structural changes in neurons after depolarization: PSD thickening (Dosemeci et al., 2001) and CaMKII clustering (Tao-Cheng et al.”
BackgroundInfluentialSelf-citationMcCollum, L. A., Walker, C. K., Roche, J. K., & Roberts, R. C. (2015) · Schizophrenia Bulletin · doi:10.1093/schbul/sbv030 · Cited by 42
Trafficking and Targeting of NMDA Receptors
Petralia, R. S., Wenthold, R. J., & Al‐Hallaq, R. A. (2008) · Frontiers in neuroscience · doi:10.1201/9781420044157.ch8 · Cited by 45
Bentea, E., Villers, A., Moore, C., Funk, A. J., O'Donovan, S. M., Verbruggen, L., Lara, O., Janssen, P., Pauw, L. D., Declerck, N. B., et al. (2020) · Molecular Psychiatry · doi:10.1038/s41380-020-0751-3 · Cited by 41
“axodendritic contacts; the width of the synaptic cleft; the area and diameter of the presynaptic terminals; the area and diameter of spine heads; the percentage of terminals containing mitochondria; the area of mitochondria and the density of mitochondria per terminal; the percentage of synapses with a perforated post…”
BackgroundPrieto, M., Folci, A., Poupon, G., Schiavi, S., Buzzelli, V., Pronot, M., François, U., Pousinha, P. A., Lattuada, N., Abélanet, S., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-21820-1 · Cited by 46
Park, C. S., Elgersma, Y., Grant, S. G. N., & Morrison, J. H. (2007) · Neuroscience · doi:10.1016/j.neuroscience.2007.09.075 · Cited by 42
“To avoid ischemic conditions resulting in PSD thickening (Dosemeci et al., 2001), the time of incision to perfusion was kept at less than 1 minute.”
Structural role of zinc ions bound to postsynaptic densities
Jan, H., Chen, I., Tsai, Y., & Chang, Y. (2002) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2002.01093.x · Cited by 40
“cross-sectional area, thickness and curvature, and the protein content of the PSD (e.g. Greenough et al. 1978; Rutledge 1978; Rees et al. 1985; Strevaag and Greenough 1985; Desmond and Levy 1986; Strack et al. 1997; Hu et al. 1998; Dosemeci et al. 2001; except for the study of Sorra and Harris 1998).”
BackgroundEpilepsy‐induced Changes in Signaling Systems of Human and Rat Postsynaptic Densities
Wyneken, Ú., Marengo, J., Villanueva, S., Soto, D., Sandoval, R., Gundelfinger, E. D., & Orrego, F. (2003) · Epilepsia · doi:10.1046/j.1528-1157.2003.17602.x · Cited by 39
Clarkson, C., Smeal, R. M., Hasenoehrl, M. G., White, J. A., Rubio, M. E., & Wilcox, K. S. (2020) · Experimental Neurology · doi:10.1016/j.expneurol.2020.113196 · Cited by 38
Activity-dependent redistribution of CaMKII in the postsynaptic compartment of hippocampal neurons
Tao-Cheng, J.-H. (2020) · Molecular Brain · doi:10.1186/s13041-020-00594-5 · Cited by 38
“these stimulation conditions may not be physiological, there are no structural damage to the cells and the effects on translocation of CaMKII are reversible [4, 5].”
Self-citationTao-Cheng, J.-H., Döṣemeci, A., Winters, C. A., & Reese, T. S. (2007) · Journal of Neurocytology · doi:10.1007/s11068-007-9012-5 · Cited by 39
“For example, immunoEM observations at the postsynaptic site show that activity causes an increase in CaMKII at the PSD ( Dosemeci et al., 2001, 2002 ).”
BackgroundSelf-citationPostsynaptic protein organization revealed by electron microscopy
Liu, Y.-T., Tao, C.-L., Lau, P.-M., Zhou, Z., & Bi, G. (2019) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2019.02.012 · Cited by 38
Döṣemeci, A., Thein, S. H., Yang, Y., Reese, T. S., & Tao-Cheng, J.-H. (2012) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2012.10.131 · Cited by 36
“cludes CaMKII and Shank1, proteins that move towards the PSD under excitatory conditions [23,27] and contribute to the ‘‘thickening’’ of the PSD [21,27].”
Self-citationWang, J., Li, C., Tu, B., Yang, K., Mo, T., Zhang, R., Cheng, S., Chen, C., Jiang, X., Han, T.-L., et al. (2018) · Toxicological Sciences · doi:10.1093/toxsci/kfy192 · Cited by 36
Pharmacological Modulation of AMPAR Rescues Intellectual Disability-Like Phenotype in Tm4sf2−/y Mice
Murru, L., Vezzoli, E., Longatti, A., Ponzoni, L., Falqui, A., Folci, A., Moretto, E., Bianchi, V., Braida, D., Sala, M., et al. (2017) · Cerebral Cortex · doi:10.1093/cercor/bhx221 · Cited by 36
“Ultrastructural analysis of excitatory synapses revealed a significant reduction in the length and thickness of postsynaptic densities (PSDs) (Dosemeci et al. 2001) in both young (Supplementary Fig.”
BackgroundResultCommons, K. G., Beck, S. G., & Bey, V. (2005) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2005.03991.x · Cited by 35
“Thickening of the postsynaptic density can be produced by exposure to glutamate (Dosemeci et al., 2001) and is thought to be associated with recruitment of proteins to the postsynaptic density.”
BackgroundPostsynaptic calcium signaling microdomains in neurons
Blackstone, C. D. (2002) · Frontiers in bioscience · doi:10.2741/a818 · Cited by 35
Nava, N., Chen, F., Wegener, G., Popoli, M., & Nyengaard, J. R. (2013) · The Journal of Comparative Neurology · doi:10.1002/cne.23482 · Cited by 35
Larsson, M., & Broman, J. (2005) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2005.04081.x · Cited by 34
“…extracellular faces of the pre- and postsynaptic plasma membranes and of the cytoplasmic face of the PSD (a modification of the procedure used by Dosemeci et al., 2001), the locations of the centre of gold particles in the pre- and postsynaptic compartments were recorded, and the different sets…”
BackgroundInfluentialSuzuki, T., Du, F., Tian, Q., Zhang, J., & Endo, S. (2007) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2007.05035.x · Cited by 33
“The cell surface CaMKII clusters may indicate synaptic sites, because CaMKII clusters accumulate in the PSD (Dosemeci et al. 2001).”
BackgroundInfluentialMultiple CaMKII Binding Modes to the Actin Cytoskeleton Revealed by Single-Molecule Imaging
Khan, S., Conte, I. L., Carter, T. D., Bayer, K. U., & Molloy, J. E. (2016) · Biophysical Journal · doi:10.1016/j.bpj.2016.06.007 · Cited by 31
Tao-Cheng, J.-H. (2019) · Molecular Brain · doi:10.1186/s13041-019-0468-x · Cited by 30
Self-citationGold, M. G. (2012) · BioEssays · doi:10.1002/bies.201200009 · Cited by 36
“07 mm(2) and a thickness of 25 nm [8, 9].”
BackgroundRuediger, J., van der Zee, E. A., Strijkstra, A. M., Aschoff, A., Daan, S., & Hut, R. A. (2007) · Synapse · doi:10.1002/syn.20380 · Cited by 32
“Under euthermic conditions, the width of a PSD is primarily determined by the amount of CaMKII incorporated, which is mainly induced by synaptic activity (Dosemeci et al., 2001; Rostas et al., 1991).”
BackgroundFlores, C. E., Cachope, R., Nannapaneni, S., Ene, S., Nairn, A. C., & Pereda, A. E. (2010) · Journal of Neuroscience · doi:10.1523/jneurosci.4466-09.2010 · Cited by 31
“In contrast to glutamatergic synapses, in which accumulation of this kinase at PSDs can be difficult sometimes to detect (Dosemeci et al., 2001; Merrill et al., 2005), the large number and intraterminal distribution of gap junctions at CEs (predominant at the center of the contact) made it easier to detect their assoc…”
BackgroundTao-Cheng, J.-H. (2018) · Molecular Brain · doi:10.1186/s13041-018-0387-2 · Cited by 30
Self-citationZhang, R., Tu, J.-B., Ran, R.-T., Zhang, W., Tan, Q., Tang, P., Kuang, T., Cheng, S., Chen, C., Jiang, X., et al. (2020) · Neurotoxicity Research · doi:10.1007/s12640-020-00286-x · Cited by 31
Wu, H., Chen, X., Shen, Z., Li, H., Liang, S., Lu, Y., & Zhang, M. (2023) · Molecular Cell · doi:10.1016/j.molcel.2023.11.011 · Cited by 33
Richardson, C. A., & Leitch, B. (2004) · The Journal of Comparative Neurology · doi:10.1002/cne.20386 · Cited by 31
“GAD67 mRNA is increased in the hippocampus following brain seizures (Feldblum et al., 1990) and, concomitantly, BDNF mRNA is induced in the same brain region (Zafra et al., 1991; Isackson et al., 1991; Dugich-Djordevic et al., 1992).”
BackgroundLendvai, B., Zelles, T., Rózsa, B. J., & Vizi, E. S. (2002) · Brain Research Bulletin · doi:10.1016/s0361-9230(02)00873-0 · Cited by 31
Tao-Cheng, J.-H., Yang, Y., Bayer, K. U., Reese, T. S., & Döṣemeci, A. (2013) · Neuroscience · doi:10.1016/j.neuroscience.2013.03.063 · Cited by 28
“In contrast, in high K+ or NMDA-treated samples where synapses become activated, CaMKII label accumulates at the PSD (Dosemeci et al., 2001; 2002).”
BackgroundSelf-citationJeanneret, V., Wu, F., Merino, P., Torre, E. R., Díaz, A., Cheng, L., & Yepes, M. (2016) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2016.00121 · Cited by 28
“…2016 | Volume 9 | Article 121 TPA Induces CaMKIIα Phosphorylation Because protein recruitment to the PSD leads to an increase in its thickness (Dosemeci et al., 2001), then we decided to use liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) to study changes in protein…”
BackgroundMethodInfluentialCalcium/calmodulin-dependent protein kinase II clusters in adult rat hippocampal slices
Tao-Cheng, J.-H., Vinade, L., Pozzo-Miller, L. D., Reese, T. S., & Dosemeci, A. (2002) · Neuroscience · doi:10.1016/s0306-4522(02)00451-7 · Cited by 32
Self-citationαCaMKII autophosphorylation levels differ depending on subcellular localization
Davies, K. D., Alvestad, R. M., Coultrap, S. J., & Browning, M. D. (2007) · Brain Research · doi:10.1016/j.brainres.2007.05.008 · Cited by 27
“Furthermore, several different forms of neuronal activation, including treatments that lead to synaptic potentiation, have been shown to induce translocation of CaMKII to post-synaptic sites (Strack et al., 1997b;Shen and Meyer, 1999;Otmakhov et al., 2004;Dosemeci et al., 2001;Bayer et al., 2001).”
BackgroundSequestration of CaMKII in dendritic spines in silico
Khan, S., Zou, Y., Amjad, A., Gardezi, A., Smith, C. L., Winters, C., & Reese, T. S. (2011) · Journal of Computational Neuroscience · doi:10.1007/s10827-011-0323-2 · Cited by 29
“Stimulus induced CaMKII binding to NR2B triggers extensive association of CaMKII with the PSD (Bayer et al. 2001; Dosemeci et al. 2001; Otmakhov et al. 2004) enabled, in part, by formation of PSD bound CaMKII aggregates.”
BackgroundSelf-citationParecoxib mitigates spatial memory impairment induced by sevoflurane anesthesia in aged rats
GONG, M., Chen, G., ZHANG, X. M., Xu, L., Wang, H. M., & Yan, M. (2012) · Acta Anaesthesiologica Scandinavica · doi:10.1111/j.1399-6576.2012.02665.x · Cited by 25
“The area of the postsynaptic density (PSD) was calculated as described previously.(20)”
MethodKuklin, E. A., Alkins, S., Bakthavachalu, B., Genco, M. C., Sudhakaran, I. P., Raghavan, K. V., Ramaswami, M., & Griffith, L. C. (2017) · Journal of Neuroscience · doi:10.1523/jneurosci.1313-17.2017 · Cited by 24
“, 2001), and activity-dependent translocation of CaMKII protein, requiring both cytoskeletal and synaptic binding partners (Shen et al., 1998; Shen and Meyer, 1999; Dosemeci et al., 2001).”
BackgroundThe importance of ultrastructural analysis of memory
Borczyk, M., Radwańska, K., & Giese, K.-P. (2021) · Brain Research Bulletin · doi:10.1016/j.brainresbull.2021.04.019 · Cited by 23
Han, X., Xiao, Y., Bao-min, A., Hu, X., Wei, Q., & Hu, Q. (2013) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.b13-00892 · Cited by 23
Han, X.-J., Shi, Z., Xia, L.-X., Zhu, L.-H., Zeng, L., Nie, J., Xu, Z., & Ruan, Y. (2016) · Neuroscience · doi:10.1016/j.neuroscience.2016.04.011 · Cited by 24
“Proteins of the postsynaptic density vary in response to synaptic activities, which in turn may lead to structural plasticity (Dosemeci et al., 2001; Meyer et al., 2014).”
Liu, S.-H., Cheng, H., Huang, S., Yiu, P.-C., & Chang, Y. (2006) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m500299-mcp200 · Cited by 23
Metadata
- Authors
- Dosemeci, A., Tao-Cheng, J.-H., Vinade, L., Winters, C. A., Pozzo-Miller, L., & Reese, T. S.
- Affiliations
- Marine Biological Laboratories, Woods Hole, MA 02543; Laboratory of Neurobiology, Electron Microscopy Facility, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892; and Department of Neurobiology, University of Alabama, Birmingham, AL 35294
- National Institutes of Health (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2001-08-21
- Volume
- 98
- Issue
- 18
- Pages
- 10428-10432
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC56977
- OpenAlex
- W2131475149
- Cited by
- 184
- References
- 24
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 4 · 2023: 7 · 2022: 6 · 2021: 3 · 2020: 18 · 2019: 4 · 2018: 8 · 2017: 6 · 2016: 13 · 2015: 7 · 2014: 6 · 2013: 5 · 2012: 5
- FWCI
- 3.33
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation (9817317)
- National Institutes of Health
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- postsynaptic density, glutamate, CaMKII, hippocampal neurons, depolarization, high potassium, synaptic plasticity, calcium/calmodulin-dependent protein kinase, calcium signaling
- MeSH terms
- Animals, Cells, Cultured, Hippocampus, Nerve Tissue Proteins, Neurons, Potassium, Microscopy, Immunoelectron, Calcium-Calmodulin-Dependent Protein Kinases, Glutamic Acid, Rats, Calcium-Calmodulin-Dependent Protein Kinase Type 2
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