Biochemical and immunochemical evidence that the "major postsynaptic density protein" is a subunit of a calmodulin-dependent protein kinase.
Open accessAbstract
By three criteria, two biochemical and one immunochemical, the major postsynaptic density protein (mPSDp) is indistinguishable from the 50-kilodalton (kDa) alpha subunit of a brain calmodulin-dependent protein kinase. First, the two proteins comigrate on NaDodSO4/polyacrylamide gels. Second, iodinated tryptic peptide maps of the two are identical. Finally, a monoclonal antibody (6G9) that was raised against the protein kinase binds on immunoblots to a single 50 kDa band in crude brain homogenates and to both the alpha subunit of the purified kinase and the mPSDp from postsynaptic density fractions. The purified kinase holoenzyme also contains a 60-kDa subunit termed beta. A comparison of the peptide map of beta with the maps of 60-kDa proteins from the postsynaptic density fraction suggests that beta is present there but is not the only protein present in this molecular weight range. These results indicate that the calmodulin-dependent protein kinase is a major constituent of the postsynaptic density fraction and thus may be a component of type I postsynaptic densities.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
CaMKII is present in the brain at extraordinarily high levels, rivaling the levels of cytoskeletal proteins.
“Second, it is present in the brain at extraordinarily high levels, rivaling the levels of cytoskeletal proteins (63–67), suggesting that CaMKII may play a structural role (27, 68).”
From Synaptic memory and CaMKII (2023) · cites this paper as [67]
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
Colonnier, M. (1968) · Brain Research · doi:10.1016/0006-8993(68)90234-5 · Cited by 946 · see citing works
Electron microscopy of excitatory and inhibitory synapses: a brief review
Gray, E. G. (1969) · Progress in Brain Research · doi:10.1016/s0079-6123(08)63235-5 · Cited by 228 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
The Postsynaptic Architecture of Excitatory Synapses: A More Quantitative View
Sheng, M., & Hoogenraad, C. C. (2007) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.76.060805.160029 · Cited by 1,003 · see citing works
SynGAP: a Synaptic RasGAP that Associates with the PSD-95/SAP90 Protein Family
Kim, J. H., Liao, D., Lau, L.-F., & Huganir, R. L. (1998) · Neuron · doi:10.1016/s0896-6273(00)81008-9 · Cited by 634 · see citing works
A Synaptic Ras-GTPase Activating Protein (p135 SynGAP) Inhibited by CaM Kinase II
Chen, H.-J., Rojas-Soto, M., Oguni, A., & Kennedy, M. B. (1998) · Neuron · doi:10.1016/s0896-6273(00)80471-7 · Cited by 590 · see citing works
Self-citationCheng, D., Hoogenraad, C. C., Rush, J., Ramm, E., Schlager, M. A., Duong, D. M., Xu, P., Wijayawardana, S. R., Hanfelt, J., Nakagawa, T., et al. (2006) · Molecular & Cellular Proteomics · doi:10.1074/mcp.d500009-mcp200 · Cited by 510 · see citing works
Why do we sleep?11Published on the World Wide Web on 7 November 2000.
Sejnowski, T. J., & Destexhe, A. (2000) · Brain Research · doi:10.1016/s0006-8993(00)03007-9 · Cited by 489 · see citing works
Yücel, Y. (2003) · Progress in Retinal and Eye Research · doi:10.1016/s1350-9462(03)00026-0 · Cited by 384 · see citing works
Takeuchi, M., Hata, Y., Hirao, K., Toyoda, A., Irie, M., & Takai, Y. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.18.11943 · Cited by 359 · see citing works
Pettit, D. L., Perlman, S., & Malinow, R. (1994) · Science · doi:10.1126/science.7997883 · Cited by 329 · see citing works
Pathways of the Past: the Imprint of Memory
Horn, G. (2004) · Nature reviews. Neuroscience · doi:10.1038/nrn1324 · Cited by 308 · see citing works
Dendritic spines: from structure to in vivo function
Rochefort, N. L., & Konnerth, A. (2012) · The EMBO Reports · doi:10.1038/embor.2012.102 · Cited by 299 · see citing works
Synaptic Signaling in Learning and Memory
Kennedy, M. B. (2016) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a016824 · Cited by 270 · see citing works
Self-citationInteractions Between Membrane Conductances Underlying Thalamocortical Slow-Wave Oscillations
DESTEXHE, A., & SEJNOWSKI, T. J. (2003) · Physiological Reviews · doi:10.1152/physrev.00012.2003 · Cited by 241 · see citing works
Liu, X. B., & Jones, E. G. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.14.7332 · Cited by 208 · see citing works
Glutamate-induced transient modification of the postsynaptic density
Dosemeci, A., Tao-Cheng, J.-H., Vinade, L., Winters, C. A., Pozzo-Miller, L., & Reese, T. S. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.181336998 · Cited by 184 · see citing works
Huntley, G., Vickers, J., Janssen, W., Brose, N., Heinemann, S., & Morrison, J. (1994) · The Journal of Neuroscience · doi:10.1523/jneurosci.14-06-03603.1994 · Cited by 161 · see citing works
Bergersen, L., Wærhaug, O., Helm, J., Thomas, M., Laake, P., Davies, A. J., Wilson, M. C., Halestrap, A. P., & Ottersen, O. P. (2001) · Experimental Brain Research · doi:10.1007/s002210000600 · Cited by 155 · see citing works
Nicoll, R. A., & Schulman, H. (2023) · Physiological Reviews · doi:10.1152/physrev.00034.2022 · Cited by 126 · see citing works
A Peptide Model for Calmodulin Trapping by Calcium/Calmodulin-dependent Protein Kinase II
Putkey, J. A., & Waxham, M. N. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.47.29619 · Cited by 94 · see citing works
Pepke, S., Kinzer-Ursem, T., Mihalas, S., & Kennedy, M. B. (2010) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1000675 · Cited by 92 · see citing works
Self-citationCA1 Long-Term Potentiation Is Diminished but Present in Hippocampal Slices from α-CaMKII Mutant Mice
Hinds, H. L., Tonegawa, S., & Malinow, R. (1998) · Learning & Memory · doi:10.1101/lm.5.4.344 · Cited by 86 · see citing works
Carlisle, H. J., Luong, T. N., Medina-Marino, A., Schenker, L., Khorosheva, E., Indersmitten, T., Gunapala, K. M., Steele, A. D., O'Dell, T. J., Patterson, P. H., et al. (2011) · The Journal of Neuroscience · doi:10.1523/jneurosci.5877-10.2011 · Cited by 74 · see citing works
Self-citationYoshimura, Y., Aoi, C., & Yamauchi, T. (2000) · Molecular Brain Research · doi:10.1016/s0169-328x(00)00170-4 · Cited by 72 · see citing works
Protein kinases involved in the expression of long-term potentiation
Tatsuo, S. (1994) · International Journal of Biochemistry · doi:10.1016/0020-711x(94)90102-3 · Cited by 52 · see citing works
Subcellular organization of camkii in rat hippocampal pyramidal neurons
Ding, J., Kennedy, M. B., & Weinberg, R. J. (2013) · Journal of Comparative Neurology · doi:10.1002/cne.23372 · Cited by 45 · see citing works
Self-citationGu, Z., & Yan, Z. (2004) · Molecular Pharmacology · doi:10.1124/mol.104.001404 · Cited by 41 · see citing works
Serotonergic neuromodulation of synaptic plasticity
Higa, G. S. V., Viana, F. J. C., Francis-Oliveira, J., Cruvinel, E., Franchin, T. S., Marcourakis, T., Ulrich, H., & De Pasquale, R. (2024) · Neuropharmacology · doi:10.1016/j.neuropharm.2024.110036 · Cited by 29 · see citing works
- Authors
- Kennedy, M. B., Bennett, M. K., & Erondu, N. E.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1983-12
- Volume
- 80
- Issue
- 23
- Pages
- 7357-7361
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC390054
- OpenAlex
- W2061893190
- Cited by
- 610
- References
- 3
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 6 · 2023: 24 · 2022: 3 · 2021: 1 · 2020: 2 · 2019: 5 · 2018: 6 · 2017: 3 · 2016: 3 · 2015: 3 · 2014: 7 · 2013: 10 · 2012: 11
- FWCI
- 9.31
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Keywords
- calcium/calmodulin-dependent protein kinase, postsynaptic density, peptide mapping
- MeSH terms
- Animals, Antibodies, Monoclonal, Antigen-Antibody Complex, Hybridomas, Molecular Weight, Nerve Tissue Proteins, Peptide Fragments, Protein Kinases, Synaptic Membranes, Trypsin, Macromolecular Substances, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
