The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion
Open accessAbstract
Producing robust and scalable fluid metering in a microfluidic device is a challenging problem. We developed a scheme for metering fluids on the picoliter scale that is scalable to highly integrated parallel architectures and is independent of the properties of the working fluid. We demonstrated the power of this method by fabricating and testing a microfluidic chip for rapid screening of protein crystallization conditions, a major hurdle in structural biology efforts. The chip has 480 active valves and performs 144 parallel reactions, each of which uses only 10 nl of protein sample. The properties of microfluidic mixing allow an efficient kinetic trajectory for crystallization, and the microfluidic device outperforms conventional techniques by detecting more crystallization conditions while using 2 orders of magnitude less protein sample. We demonstrate that diffraction-quality crystals may be grown and harvested from such nanoliter-volume reactions.
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Cited by
The origins and the future of microfluidics
Whitesides, G. M. (2006) · Nature · doi:10.1038/nature05058 · Cited by 9,687
Small-scale systems for in vivo drug delivery
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Microfluidic Devices for Bioapplications
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Microfluidic Device for Single-Cell Analysis
Wheeler, A. R., Throndset, W. R., Whelan, R. J., Leach, A. M., Zare, R. N., Liao, Y. H., Farrell, K., Manger, I. D., & Daridon, A. (2003) · Analytical Chemistry · doi:10.1021/ac0340758 · Cited by 600
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Metadata
- Authors
- Hansen, C. L., Skordalakes, E., Berger, J. M., & Quake, S. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2002-12-16
- Volume
- 99
- Issue
- 26
- Pages
- 16531-16536
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2078863041
- Cited by
- 517
- References
- 24
- FWCI
- 22.71
- Type
- journal article
- Language
- en
- Open access
- green
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- Hansen, C. L., Skordalakes, E., Berger, J. M., & Quake, S. R. (2002). A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.262485199
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
