The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Degradation of cationized low density lipoprotein and regulation of cholesterol metabolism in homozygous familial hypercholesterolemia fibroblasts.
Open accessAbstract
Cultured fibroblasts derived from patients with homozygous familial hypercholesterolemia, which lack functional low density lipoprotein (LDL) receptors, fail to bind, take up, or degrade the lipoprotein with high affinity; therefore LDL-cholesterol is not made available for suppression of cholesterol synthesis or activation of cholesteryl ester formation. When LDL was given a positive charge by reaction with N,N-dimethyl-1,3-propanediamine (cationized LDL), the rate of degradation of the lipoprotein was increased by more than 100-fold in the homozygous familial hypercholesterolemia fibroblasts. Degradation of cationized LDL was inhibited by chloroquine, suggesting that it occurred in cellular lysosomes. Although the cationized LDL entered the cell through a mechanism independent of the LDL receptor, the cholesterol liberated from the degradation of the lipoprotein became available for suppression of cholesterol synthesis and stimulation of cholesteryl ester formation in the homozygous familial hypercholesterolemia fibroblasts. The rate of degradation of albumin by fibroblasts was also increased by more than 100-fold when this protein was coupled to N,N-dimethyl-1,3-propanediamine. The ability to deliver a protein to lysosomes by giving it a strong positive charge may have potential relevance not only to familial hypercholesterolemia, but also to inborn errors of metabolism that involve deficiencies in lysosomal enzymes.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 317,331
Electrophoretic separation of plasma lipoproteins in agarose gel
Noble, R. P. (1968) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)42680-x · Cited by 1,307
Binding and Degradation of Low Density Lipoproteins by Cultured Human Fibroblasts
Goldstein, J. L., & Brown, M. S. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42341-7 · Cited by 1,123
Self-citationBrown, M. S., & Goldstein, J. L. (1976) · Science · doi:10.1126/science.174194 · Cited by 725
Self-citationUse of cationized ferritin as a label of negative charges on cell surfaces
Danon, D., Goldstein, L., Marikovsky, Y., & Skutelsky, E. (1972) · Journal of Ultrastructure Research · doi:10.1016/0022-5320(72)90087-1 · Cited by 622
Release of low density lipoprotein from its cell surface receptor by sulfated glycosaminoglycans
Goldstein, J. L., Basu, S. K., Brunschede, G. Y., & Brown, M. S. (1976) · Cell · doi:10.1016/0092-8674(76)90258-0 · Cited by 613
Self-citationBrown, M. S., & Goldstein, J. L. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.3.788 · Cited by 596
Self-citationBlood Lipids and Lipoproteins: Quantitation, Composition and Metabolism
(1973) · Medical Journal of Australia · doi:10.5694/j.1326-5377.1973.tb128705.x · Cited by 554
Regulation of 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase Activity in Cultured Human Fibroblasts
Brown, M. S., Dana, S. E., & Goldstein, J. L. (1974) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)42998-0 · Cited by 544
Self-citationRegulation of the activity of the low density lipoprotein receptor in human fibroblasts
Brown, M. S., & Goldstein, J. L. (1975) · Cell · doi:10.1016/0092-8674(75)90182-8 · Cited by 482
Self-citation
Cited by
A Receptor-Mediated Pathway for Cholesterol Homeostasis
Brown, M. S., & Goldstein, J. L. (1986) · Science · doi:10.1126/science.3513311 · Cited by 5,703
Self-citationReceptor-Mediated Endocytosis: Concepts Emerging from the LDL Receptor System
Goldstein, J. L., Brown, M. S., Anderson, R. G. W., Russell, D. W., & Schneider, W. J. (1985) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.01.110185.000245 · Cited by 1,600
Self-citationThe human LDL receptor: A cysteine-rich protein with multiple Alu sequences in its mRNA
Yamamoto, T., Davis, C. G., Brown, M. S., Schneider, W. J., Casey, M. L., Goldstein, J. L., & Russell, D. W. (1984) · Cell · doi:10.1016/0092-8674(84)90188-0 · Cited by 1,382
Self-citationVoyta, J. C., Via, D. P., Butterfield, C. E., & Zetter, B. R. (1984) · The Journal of cell biology · doi:10.1083/jcb.99.6.2034 · Cited by 1,239
Chinetti, G., Lestavel, S., Bocher, V., Remaley, A. T., Neve, B., Torra, I. P., Teissier, E., Minnich, A., Jaye, M., Duverger, N., et al. (2001) · Nature Medicine · doi:10.1038/83348 · Cited by 1,170
Metadata
- Authors
- Basu, S. K., Goldstein, J. L., Anderson, G. W., & Brown, M. S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1976-09
- Volume
- 73
- Issue
- 9
- Pages
- 3178-3182
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2120626817
- Cited by
- 1,021
- References
- 20
- FWCI
- 16.66
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Degradation of cationized low density lipoprotein and regulation of cholesterol metabolism in homozygous familial hypercholesterolemia fibroblasts. https://app.cochat.ai/papers/10.1073/pnas.73.9.3178
- The paper
- Basu, S. K., Goldstein, J. L., Anderson, G. W., & Brown, M. S. (1976). Degradation of cationized low density lipoprotein and regulation of cholesterol metabolism in homozygous familial hypercholesterolemia fibroblasts. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.73.9.3178
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
