Cited by 244Adipose Tissue and MetabolismMitochondrial Function and PathologyPeroxisome Proliferator-Activated Receptors

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Molecular approach to thermogenesis in brown adipose tissue: cDNA cloning of the mitochondrial uncoupling protein.

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Bouillaud, F, Ricquier, D, Thibault, J, Weissenbach, J·1985·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.82.2.445PMC PMC397055

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Abstract

The uncoupling protein (UCP) of mammalian brown fat is a specialized and unique component responsible for energy dissipation as heat. Translation and immunoprecipitation from sucrose-fractionated mRNA indicated that the mRNA of UCP sedimented at 14-16 S. A recombinant cDNA library prepared from mRNA of thermoactive brown fat enriched for UCP mRNA has been constructed and cloned in Escherichia coli. Recombinant plasmids were screened by differential colony hybridization to a cDNA probe complementary to poly(A)+ RNA isolated from thermogenic or from weakly thermogenic brown fat. Several differentially hybridizing plasmids were shown to contain UCP cDNA sequences by their ability to select a mRNA coding for an in vitro translation product that was immunoprecipitable with antibodies against UCP. Blot hybridization of brown fat mRNA to a 32P-labeled UCP cDNA probe revealed two major species of mRNA (15S and 18S). As compared to non-thermogenic tissue, a strikingly increased hybridization to the probe was observed with brown fat mRNA from thermoactive tissue. Moreover, hybridization was observed with RNA of brown adipose tissue from rat, hamster, or mouse but not with RNA from rat or mouse liver.

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Detection of specific sequences among DNA fragments separated by gel electrophoresis
Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,245
Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
Aviv, H., & Leder, P. (1972) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.69.6.1408 · Cited by 6,636
An Efficient mRNA‐Dependent Translation System from Reticulocyte Lysates
Pelham, H. R. B., & Jackson, R. J. (1976) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1976.tb10656.x · Cited by 3,926
RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination
Lehrach, H. R., Diamond, D. J., Wozney, J. M., & Boedtker, H. (1977) · Biochemistry · doi:10.1021/bi00640a033 · Cited by 3,415
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
Grunstein, M. R., & Hogness, D. S. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.10.3961 · Cited by 3,386
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
Wahl, G. M., Stern, M., & Stark, G. R. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.8.3683 · Cited by 3,159
Rapid and efficient cosmid cloning
Ish‐Horowicz, D., & Burke, J. F. (1981) · Nucleic Acids Research · doi:10.1093/nar/9.13.2989 · Cited by 1,757
Thermogenic mechanisms in brown fat.
Nicholls, D. G., & Locke, R. M. (1984) · Physiological Reviews · doi:10.1152/physrev.1984.64.1.1 · Cited by 1,602
Construction and characterization of new cloning vehicles IV. Deletion derivatives of pBR322 and pBR325
Soberón, X., Covarrubias, L., & Bolívar, F. (1980) · Gene · doi:10.1016/0378-1119(90)90328-o · Cited by 612
Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.
Goldberg, D. A. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.10.5794 · Cited by 571

Show all 27 references

Research works printed but not linked: 8

[12]Lomedico, P. T. & Saunders, G. F. (1976) Nucleic Acids Res. 3, 381–391.
[13]Aviv, H. & Leder, P. (1972) Proc. Natl. Acad. Sci. USA 69, 1408-1412.
[14]Schweinfest, C. W., Kwiatkowski, R. W. & Dottin, R. P. (1982) Proc. Natl. Acad. Sci. USA 79, 4997–5000.
[17]Rougeon, F. & Mach, B. (1976) Proc. Natl. Acad. Sci. USA 73, 3418–3422.
[21]Grunstein, M. & Hogness, D. (1975) Proc. Natl. Acad. Sci. USA 72, 3961–3965.
[23]Ricciardi, R. P., Miller, J. S. & Roberts, B. E. (1979) Proc. Natl. Acad. Sci. USA 76, 4927–4931.
[25]Goldberg, D. A. (1980) Proc. Natl. Acad. Sci. USA 77, 5794– 5798.
[27]Wahl, G. M., Stern, M. & Stark, G. R. (1979) Proc. Natl. Acad. Sci. USA 76, 3683–3687.

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Aréchaga, I., Raimbault, S., Prieto, S., Lévi-Meyrueis, C., Zaragoza, P., Miroux, B., Ricquier, D., Bouillaud, F., & Rial, E. (1993) · Biochemical Journal · doi:10.1042/bj2960693 · Cited by 86
Complete nucleotide and derived amino acid sequence of cDNA encoding the mitochondrial uncoupling protein of rat brown adipose tissue: lack of a mitochondrial targeting presequence
Ridley, R. G., Patel, H. V., Gerber, G. E., Morton, R. C., & Freeman, K. B. (1986) · Nucleic Acids Research · doi:10.1093/nar/14.10.4025 · Cited by 85
Uncoupling proteins-2 and 3 influence obesity and inflammation in transgenic mice
Horváth, T. L., Diano, S., Miyamoto, S., Barry, S. J., Gatti, S., Alberati, D., Livák, F., Lombardi, A., Moreno, M., Goglia, F., et al. (2003) · International Journal of Obesity · doi:10.1038/sj.ijo.0802257 · Cited by 84
Triiodothyronine Induces the Transcription of the Uncoupling Protein Gene and Stabilizes Its mRNA in Fetal Rat Brown Adipocyte Primary Cultures
Guerra, C. E., Roncero, C., Porrás, A., Fernández, M., & Benito, M. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.4.2076 · Cited by 81
Induction of UCP2 Gene Expression by LPS: A Potential Mechanism for Increased Thermogenesis during Infection
Faggioni, R., Shigenaga, J. K., Moser, A. H., Feingold, K. R., & Grünfeld, C. (1998) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.8219 · Cited by 80
Mitochondrial uncoupling protein 2 in the central nervous system: neuromodulator and neuroprotector
Horváth, T. L., Diano, S., & Barnstable, C. J. (2003) · Biochemical Pharmacology · doi:10.1016/s0006-2952(03)00143-6 · Cited by 81
Ontogeny and perinatal modulation of gene expression in rat brown adipose tissue
Giralt, M., Martin, I., Iglesias, R., Viñas, O., Villarroya, F., & Mampel, T. (1990) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1990.tb19336.x · Cited by 78
Multiple symmetric lipomatosis may be the consequence of defective noradrenergic modulation of proliferation and differentiation of brown fat cells
Nisoli, E., Regianini, L., Briscini, L., Bulbarelli, A., Busetto, L., Coin, A., Enzi, G., & Carruba, M. O. (2002) · The Journal of Pathology · doi:10.1002/path.1212 · Cited by 75
Leptin Affects Pancreatic Endocrine Functions through the Sympathetic Nervous System 1
Mizuno, A., Murakami, T., Otani, S., Kuwajima, M., & Shima, K. (1998) · Endocrinology · doi:10.1210/endo.139.9.6201 · Cited by 77
Phosphatidylinositol 3-Kinase Is a Requirement for Insulin-Like Growth Factor I-Induced Differentiation, but not for Mitogenesis, in Fetal Brown Adipocytes
Valverde, Á. M., Lorenzo, M., Navarro, P., & Benito, M. (1997) · Molecular Endocrinology · doi:10.1210/mend.11.5.9924 · Cited by 75
Essential cis-Acting Elements in Rat Uncoupling Protein Gene Are in an Enhancer Containing a Complex Retinoic Acid Response Domain
Larose, M., Cassard, A., Fleury, C., Serra, F. A., Champigny, O., Bouillaud, F., & Ricquier, D. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.49.31533 · Cited by 70
Adipose tissues from various anatomical sites are characterized by different patterns of gene expression and regulation
Cousin, B., Casteilla, L., Dani, C., Muzzin, P., Revelli, J. P., & Pénicaud, L. (1993) · Biochemical Journal · doi:10.1042/bj2920873 · Cited by 70
New Aspects of Mitochondrial Uncoupling Proteins (UCPs) and Their Roles in Tumorigenesis
Robbins, D. F., & Zhao, Y. (2011) · International Journal of Molecular Sciences · doi:10.3390/ijms12085285 · Cited by 69
Plant Uncoupling Mitochondrial Protein and Alternative Oxidase: Energy Metabolism and Stress
Borecký, J., & Vercesi, A. E. (2005) · Bioscience Reports · doi:10.1007/s10540-005-2889-2 · Cited by 70
Targeting Intermediary Metabolism in the Hypothalamus as a Mechanism to Regulate Appetite
Lopaschuk, G. D., Ussher, J. R., & Jaswal, J. S. (2010) · Pharmacological Reviews · doi:10.1124/pr.109.002428 · Cited by 71
Dynamics of volume transmission in the brain. Focus on catecholamine and opioid peptide communication and the role of uncoupling protein 2
Fuxé, K., Rivera, A., Jacobsen, K. X., H�istad, M., Leo, G., Horváth, T. L., Staines, W. A., de la Calle, A., & Agnati, L. F. (2004) · Journal of Neural Transmission · doi:10.1007/s00702-004-0158-3 · Cited by 69
α- and β-adrenergic control of thermogenin mRNA expression in brown adipose tissue
Jacobsson, A., Nedergaard, J., & Cannon, B. (1986) · Bioscience Reports · doi:10.1007/bf01114756 · Cited by 68
Regulation of Feeding-Associated Peptides and Receptors by Nicotine
Li, M. D., Parker, S. L., & Kane, J. K. (2000) · Molecular Neurobiology · doi:10.1385/mn:22:1-3:143 · Cited by 65
Tumor Necrosis Factor- Causes Insulin Receptor Substrate-2-Mediated Insulin Resistance and Inhibits Insulin-Induced Adipogenesis in Fetal Brown Adipocytes
Valverde, Á. M., Teruel, T., Navarro, P., Benito, M., & Lorenzo, M. (1998) · Endocrinology · doi:10.1210/endo.139.3.5854 · Cited by 66
Disruption of klotho Gene Causes an Abnormal Energy Homeostasis in Mice
Mori, K., Yahata, K., Mukoyama, M., Suganami, T., Makino, H., Nagae, T., Masuzaki, H., Ogawa, Y., Sugawara, A., Nabeshima, Y.-I., et al. (2000) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2000.3864 · Cited by 65
Opposite regulation of PPAR-α and -γ gene expression by both their ligands and retinoic acid in brown adipocytes
Valmaseda, A., Carmona, M. C., Barberà, M. J., Viñas, O., Mampel, T., Iglesias, R., Villarroya, F., & Giralt, M. (1999) · Molecular and Cellular Endocrinology · doi:10.1016/s0303-7207(99)00081-7 · Cited by 63
Insulin and Contraction Directly Stimulate UCP2 and UCP3 mRNA Expression in Rat Skeletal Muscle in Vitro
Pedersen, S. B., Lund, S. A., Buhl, E. S., & Richelsen, B. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.4736 · Cited by 63
Mitochondrial uncoupling as a potential therapeutic target in acute central nervous system injury
Maragos, W. F., & Korde, A. S. (2004) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2004.02736.x · Cited by 64
Dominant Negative Regulation by c-Jun of Transcription of the Uncoupling Protein-1 Gene through a Proximal cAMP-Regulatory Element: A Mechanism for Repressing Basal and Norepinephrine-Induced Expression of the Gene before Brown Adipocyte Differentiation
Yubero, P., Barberá, M., Alvarez, R., Viñas, O., Mampel, T., Iglesias, R., Villarroya, F., & Giralt, M. (1998) · Molecular Endocrinology · doi:10.1210/mend.12.7.0137 · Cited by 63
The uncoupling proteins.
Muzzin, P. (2002) · PubMed · Cited by 63
Cell Fate and Differentiation of Bone Marrow Mesenchymal Stem Cells
Kokabu, S., Lowery, J. W., & Jimi, E. (2016) · Stem Cells International · doi:10.1155/2016/3753581 · Cited by 65
Nonshivering Thermogenesis in a Marsupial (the Tasmanian Bettong Bettongia gaimardi) Is Not Attributable to Brown Adipose Tissue
Rose, R. W., West, A. K., Ye, J., McCormack, G. H., & Colquhoun, E. Q. (1999) · Physiological and Biochemical Zoology · doi:10.1086/316709 · Cited by 60

Authors: Bouillaud, F., Ricquier, D., Thibault, J., & Weissenbach, J.
Affiliations: Centre National de la Recherche Scientifique (FR)
ORCID: Bouillaud, F
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1985-01
Volume: 82
Issue: 2
Pages: 445-448
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.82.2.445
PMC: PMC397055
OpenAlex: W2088076536
Cited by: 244
References: 27
Citations by year: 2026: 4 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 8 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 6 · 2015: 4 · 2014: 3 · 2013: 6 · 2012: 6
FWCI: 5.45
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Centre National de la Recherche Scientifique
Topics: Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Keywords: uncoupling proteins, brown adipose tissue, thermogenesis, cDNA cloning, mitochondrial uncoupling, energy dissipation, tissue-specific gene expression, rats, hamster, mice
MeSH terms: Uncoupling Protein 1, Animals, Body Temperature Regulation, Adipose Tissue, Brown, Carrier Proteins, Centrifugation, Density Gradient, Cloning, Molecular, DNA, Electrophoresis, Polyacrylamide Gel, Ion Channels, Male, Membrane Proteins, Nucleic Acid Hybridization, Plasmids, Rats, Inbred Strains, RNA, Messenger, Mitochondrial Proteins, Rats

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Molecular approach to thermogenesis in brown adipose tissue: cDNA cloning of the mitochondrial uncoupling protein.

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