Cloning, characterization, expression, and chromosomal localization of a human ferritin heavy-chain gene
Open accessAbstract
A genomic phage clone containing a full-length copy of a functional human gene for ferritin heavy chain has been isolated. The gene consists of four exons spanning approximately 3 kilobases and has been localized to chromosome 11. The functionality of the gene was demonstrated by the fact that both transient transfectants and stable transformants of murine fibroblasts actively transcribe human ferritin heavy-chain mRNA.
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- Authors
- Hentze, M. W., Keim, S., Papadopoulos, P., O'Brien, S., Modi, W., Drysdale, J., Leonard, W. J., Harford, J. B., & Klausner, R. D.
- Affiliations
- Tufts University (US)
- National Institutes of Health (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1986-10
- Volume
- 83
- Issue
- 19
- Pages
- 7226-7230
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC386688
- OpenAlex
- W2009900798
- Cited by
- 79
- References
- 31
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 2 · 2019: 2 · 2017: 1 · 2016: 1 · 2015: 1 · 2013: 1
- FWCI
- 5.60
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- NSUWorks (Nova Southeastern University) (submittedVersion)
- Funders
- National Institutes of Health (5r01am017775-12)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Neuroscience › Neurology › Neurological diseases and metabolism
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Keywords
- FTH1, gene cloning, gene structure, exon-intron organization, gene expression, transient transfection, stable transfection, mouse fibroblasts, human genes
- MeSH terms
- Amino Acid Sequence, Base Sequence, Chromosomes, Human, Pair 11, Cloning, Molecular, DNA Restriction Enzymes, Ferritins, Gene Expression Regulation, Genes, Humans, Transcription, Genetic, Transfection
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