Cited by 79Iron Metabolism and DisordersNeurological diseases and metabolismTrace Elements in Health

Cloning, characterization, expression, and chromosomal localization of a human ferritin heavy-chain gene

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Hentze, M W, Keim, S, Papadopoulos, P, O'Brien, S, Modi, W, Drysdale, J, Leonard, W J, Harford, J B, Klausner, R D·1986·Proceedings of the National Academy of Sciences

Abstract

A genomic phage clone containing a full-length copy of a functional human gene for ferritin heavy chain has been isolated. The gene consists of four exons spanning approximately 3 kilobases and has been localized to chromosome 11. The functionality of the gene was demonstrated by the fact that both transient transfectants and stable transformants of murine fibroblasts actively transcribe human ferritin heavy-chain mRNA.

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Crossref · 10.1073/pnas.83.19.7226

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31 of 31 references verified0 citation errors · 0 not checkable

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Authors
Hentze, M. W., Keim, S., Papadopoulos, P., O'Brien, S., Modi, W., Drysdale, J., Leonard, W. J., Harford, J. B., & Klausner, R. D.
Affiliations
  • Tufts University (US)
  • National Institutes of Health (US)
Venue
Proceedings of the National Academy of Sciences
Published
1986-10
Volume
83
Issue
19
Pages
7226-7230
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2009900798
Cited by
79
References
31
Citations by year
2025: 1 · 2023: 1 · 2022: 2 · 2019: 2 · 2017: 1 · 2016: 1 · 2015: 1 · 2013: 1
FWCI
5.60
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • NSUWorks (Nova Southeastern University) (submittedVersion)
Funders
National Institutes of Health (5r01am017775-12)
Topics
  • Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
  • Life Sciences › Neuroscience › Neurology › Neurological diseases and metabolism
  • Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
Keywords
FTH1, gene cloning, gene structure, exon-intron organization, gene expression, transient transfection, stable transfection, mouse fibroblasts, human genes
MeSH terms
Amino Acid Sequence, Base Sequence, Chromosomes, Human, Pair 11, Cloning, Molecular, DNA Restriction Enzymes, Ferritins, Gene Expression Regulation, Genes, Humans, Transcription, Genetic, Transfection

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