Translational repression by a complex between the iron‐responsive element of ferritin mRNA and its specific cytoplasmic binding protein is position‐dependent in vivo.
Open accessAbstract
The interaction of ferritin mRNA is regulated by iron via the interaction of a cytoplasmic binding protein (IRE-BP) with a specific stem-loop structure in the 5' untranslated region (UTR), referred to as the iron-responsive element (IRE). A high affinity RNA-protein complex between the IRE and the IRE-BP functions as a repressor of translation in vivo. Translational repression appears to depend upon the position of the IRE in the 5' UTR of the mRNA. IREs located in the 5' untranslated region 67 nucleotides or more downstream of the 5' terminus of the mRNA fail to mediate iron-dependent translational regulation and give rise to constitutively derepressed transcripts. A model is proposed in which translational regulation of protein biosynthesis involves a position-dependent interference of the IRE/IRE-BP complex with one of the initial steps in translation initiation.
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- Authors
- Goossen, B., Caughman, S. W., Harford, J. B., Klausner, R. D., & Hentze, M. W.
- Affiliations
- European Molecular Biology Laboratory (DE)
- Venue
- The EMBO Journal
- Published
- 1990-12-01
- Volume
- 9
- Issue
- 12
- Pages
- 4127-4133
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC552186
- OpenAlex
- W979668760
- Cited by
- 144
- References
- 49
- Citations by year
- 2024: 3 · 2023: 1 · 2022: 3 · 2021: 2 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 8.98
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- The EMBO Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- protein synthesis inhibition, iron-responsive element, ferritin mRNA, iron-responsive element-binding protein, 5' untranslated region, protein-RNA complex, translation initiation, stem-loop structure
- MeSH terms
- Animals, Base Sequence, Binding Sites, Carrier Proteins, Cell Line, Cytoplasm, Ferritins, Gene Expression Regulation, Molecular Sequence Data, Nucleic Acid Conformation, Plasmids, RNA, RNA, Messenger, Transcription, Genetic, Transfection, Protein Biosynthesis, Blotting, Northern, Restriction Mapping, Oligonucleotide Probes, Mutagenesis, Insertional, Iron-Regulatory Proteins, Mice
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