Cited by 144Iron Metabolism and DisordersRNA and protein synthesis mechanismsRNA Research and Splicing

Translational repression by a complex between the iron‐responsive element of ferritin mRNA and its specific cytoplasmic binding protein is position‐dependent in vivo.

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Goossen, B., Caughman, S. W., Harford, J. B., Klausner, R. D., Hentze, M. W.·1990·The EMBO Journal

Abstract

The interaction of ferritin mRNA is regulated by iron via the interaction of a cytoplasmic binding protein (IRE-BP) with a specific stem-loop structure in the 5' untranslated region (UTR), referred to as the iron-responsive element (IRE). A high affinity RNA-protein complex between the IRE and the IRE-BP functions as a repressor of translation in vivo. Translational repression appears to depend upon the position of the IRE in the 5' UTR of the mRNA. IREs located in the 5' untranslated region 67 nucleotides or more downstream of the 5' terminus of the mRNA fail to mediate iron-dependent translational regulation and give rise to constitutively derepressed transcripts. A model is proposed in which translational regulation of protein biosynthesis involves a position-dependent interference of the IRE/IRE-BP complex with one of the initial steps in translation initiation.

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Crossref · 10.1002/j.1460-2075.1990.tb07635.x

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Authors
Goossen, B., Caughman, S. W., Harford, J. B., Klausner, R. D., & Hentze, M. W.
Affiliations
European Molecular Biology Laboratory (DE)
Venue
The EMBO Journal
Published
1990-12-01
Volume
9
Issue
12
Pages
4127-4133
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
OpenAlex
W979668760
Cited by
144
References
49
Citations by year
2024: 3 · 2023: 1 · 2022: 3 · 2021: 2 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 1
FWCI
8.98
Type
journal article
Language
en
Open access
Locations
  • The EMBO Journal (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords
protein synthesis inhibition, iron-responsive element, ferritin mRNA, iron-responsive element-binding protein, 5' untranslated region, protein-RNA complex, translation initiation, stem-loop structure
MeSH terms
Animals, Base Sequence, Binding Sites, Carrier Proteins, Cell Line, Cytoplasm, Ferritins, Gene Expression Regulation, Molecular Sequence Data, Nucleic Acid Conformation, Plasmids, RNA, RNA, Messenger, Transcription, Genetic, Transfection, Protein Biosynthesis, Blotting, Northern, Restriction Mapping, Oligonucleotide Probes, Mutagenesis, Insertional, Iron-Regulatory Proteins, Mice

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