The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index; none of the 2 sources its claims cite is checked for support yet.
BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death.
Open accessAbstract
The BCL2 protooncogene encodes an inner mitochondrial membrane protein that blocks programmed cell death. BCL2 was isolated from the chromosomal breakpoint of follicular B-cell lymphoma. Transgenic mice that overexpress BCL2 display extended survival of resting B cells. In this study we use a monospecific anti-human BCL2 antibody to define the distribution of BCL2 protein within organized tissues. BCL2 is restricted within germinal centers to the follicular mantle and to portions of the light zone implicated in the selection and maintenance of plasma cells and memory B cells. BCL2 is present in the surviving T cells in the thymic medulla. All hematopoietic lineages that derive from a renewing stem cell also display BCL2. A limited number of nonlymphoid tissues demonstrate BCL2 and can be grouped as (i) glandular epithelium in which hormones or growth factors regulate hyperplasia and involution, (ii) complex differentiating epithelium such as skin and intestine characterized by long-lived stem cells, and (iii) long-lived postmitotic cells such as neurons. Within these tissues that demonstrate apoptotic cell turnover, BCL2 is often topographically restricted to long-lived or proliferating cell zones. BCL2's function as an antidote to apoptosis may confer longevity to progenitor and effector cells in these tissues.
Overview
AI snapshot
Written from the paper’s full text.
TL;DR
Using a monospecific anti-human BCL2 antibody, the authors map BCL2 protein across organized tissues and find it topographically restricted to long-lived or proliferating zones in tissues with apoptotic turnover. BCL2 is present in selected lymphoid, hematopoietic, glandular epithelial, complex epithelial, and postmitotic neuronal compartments.
Key findings
- Within germinal centers, BCL2 is restricted to the follicular mantle and portions of the light zone implicated in selection and maintenance of plasma cells and memory B cells.
- BCL2 is present in surviving T cells in the thymic medulla and in all hematopoietic lineages derived from a renewing stem cell.
- A limited set of nonlymphoid tissues shows BCL2: glandular epithelium regulated by hormones/growth factors, complex differentiating epithelium such as skin and intestine with long-lived stem cells, and long-lived postmitotic cells such as neurons.
- In tissues with apoptotic cell turnover, BCL2 is often topographically restricted to long-lived or proliferating cell zones; the authors suggest BCL2 may confer longevity to progenitor and effector cells.
Methods
- The study uses a monospecific anti-human BCL2 antibody to define BCL2 protein distribution within organized tissues.
- The provided text does not report sample sizes, tissue sources, staining protocols, or quantitative analysis details.
Limitations
- The paper’s abstract does not state limitations.
- Evident from the provided text: only the abstract and reference list are available, so sample composition, antibody specificity controls, quantification, and full results cannot be assessed. No quantitative values are reported in the provided text.
Model-written summary. Check it against the paper before relying on it.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
4 Own interpretation · 4 Own result · 2 No full text of the sources · 1 No basis given
The paper states this without a source or data behind it here, so there is nothing to check it against. The quote shows it is in the paper.
In the paper
“All hematopoietic lineages that derive from a renewing stem cell also display BCL2.”
plaincorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Rests on an assumption: The anti-human BCL2 antibody used in this study is monospecific for BCL2.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“BCL2 is present in the surviving T cells in the thymic medulla.”
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Rests on an assumption: The anti-human BCL2 antibody used in this study is monospecific for BCL2.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“A limited number of nonlymphoid tissues demonstrate BCL2”
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Rests on an assumption: The anti-human BCL2 antibody used in this study is monospecific for BCL2.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“BCL2 is restricted within germinal centers to the follicular mantle and to portions of the light zone implicated in the selection and maintenance of plasma cells and memory B cells.”
plain- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Rests on an assumption: The anti-human BCL2 antibody used in this study is monospecific for BCL2.
A result the paper reports from its own data — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“Within these tissues that demonstrate apoptotic cell turnover, BCL2 is often topographically restricted to long-lived or proliferating cell zones.”
hedgedcorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Rests on an assumption: The anti-human BCL2 antibody used in this study is monospecific for BCL2.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“glandular epithelium in which hormones or growth factors regulate hyperplasia and involution”
plaincorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Rests on an assumption: The anti-human BCL2 antibody used in this study is monospecific for BCL2.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“long-lived postmitotic cells such as neurons”
plaincorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Rests on an assumption: The anti-human BCL2 antibody used in this study is monospecific for BCL2.
In the paper
“Transgenic mice that overexpress BCL2 display extended survival of resting B cells.”
What the sources say
- Not checked — no full text of the sourcecites McDonnell et al., 1989 source
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“complex differentiating epithelium such as skin and intestine characterized by long-lived stem cells”
plaincorrelational- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Rests on an assumption: The anti-human BCL2 antibody used in this study is monospecific for BCL2.
A conclusion the paper draws from its own results — it cites no other source for it, so there is none to check. The quote shows it is in the paper.
In the paper
“BCL2's function as an antidote to apoptosis may confer longevity to progenitor and effector cells in these tissues.”
hedgedconditional- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
- Rests on an assumption: The anti-human BCL2 antibody used in this study is monospecific for BCL2.
- It may state two separate claims as one. 1
In the paper
“The BCL2 protooncogene encodes an inner mitochondrial membrane protein that blocks programmed cell death.”
What the sources say
- Not checked — no full text of the sourcecites Hockenbery et al., 1990 source
plaindirect- Asserted: passes — The authors put it forward as true.
- Checkable: passes — It can be checked against evidence.
- Load-bearing: passes — The paper’s conclusions depend on it.
- Based: passes — The paper gives a reason why it holds.
It may state two separate claims as one.
The paper relies on this without support.
The anti-human BCL2 antibody used in this study is monospecific for BCL2.
“we use a monospecific anti-human BCL2 antibody”
Citations
Cites
Apoptosis: A Basic Biological Phenomenon with Wideranging Implications in Tissue Kinetics
Kerr, J. F. R., Wyllie, A. H., & Currie, A. R. (1972) · British Journal of Cancer · doi:10.1038/bjc.1972.33 · Cited by 15,673
Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death
Hockenbery, D., Nuñez, G., Milliman, C., Schreiber, R. D., & Korsmeyer, S. J. (1990) · Nature · doi:10.1038/348334a0 · Cited by 3,720
Self-citationBcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells
Vaux, D., Cory, S., & Adams, J. (1988) · Nature · doi:10.1038/335440a0 · Cited by 3,355
Cheng, H., & Leblond, C. P. (1974) · American Journal of Anatomy · doi:10.1002/aja.1001410407 · Cited by 1,737
Savill, J. S., Wyllie, A. H., Henson, J. E., Walport, M. J., Henson, P. M., & Haslett, C. (1989) · Journal of Clinical Investigation · doi:10.1172/jci113970 · Cited by 1,536
Cohen, J., & Duke, R. C. (1984) · The Journal of Immunology · doi:10.4049/jimmunol.132.1.38 · Cited by 1,406
Regressive Events in Neurogenesis
Cowan, W. M., Fawcett, J. W., O'Leary, D. D. M., & Stanfield, B. B. (1984) · Science · doi:10.1126/science.6474175 · Cited by 1,316
McDonnell, T. J., Deane, N., Platt, F. M., Nunez, G., Jaeger, U., McKearn, J. P., & Korsmeyer, S. J. (1989) · Cell · doi:10.1016/0092-8674(89)90174-8 · Cited by 1,213
Self-citationCleary, M. L., Smith, S. D., & Sklar, J. (1986) · Cell · doi:10.1016/0092-8674(86)90362-4 · Cited by 1,212
Bakhshi, A., Jensen, J. P., Goldman, P., Wright, J. J., McBride, O. W., Epstein, A. L., & Korsmeyer, S. J. (1985) · Cell · doi:10.1016/s0092-8674(85)80070-2 · Cited by 1,177
Self-citationTsujimoto, Y., Gorham, J. D., Cossman, J., Jaffe, E. S., & Croce, C. M. (1985) · Science · doi:10.1126/science.3929382 · Cited by 990
Cleary, M. L., & Sklar, J. L. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.21.7439 · Cited by 955
Martin, D. P., Schmidt, R. E., DiStefano, P. S., Lowry, O. H., Carter, J. G., & Johnson, E. M. (1988) · The Journal of Cell Biology · doi:10.1083/jcb.106.3.829 · Cited by 901
Nuñez, G., London, L., Hockenbery, D., Alexander, M., McKearn, J. P., & Korsmeyer, S. J. (1990) · The Journal of Immunology · doi:10.4049/jimmunol.144.9.3602 · Cited by 899
Self-citationMcDonnell, T. J., & Korsmeyer, S. J. (1991) · Nature · doi:10.1038/349254a0 · Cited by 872
Self-citationExpression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation.
Pezzella, F., Tse, A. G. D., Cordell, J. L., Pulford, K. A. F., Gatter, K. C., & Mason, D. Y. (1990) · Europe PMC (PubMed Central) · Cited by 595
The Role of the T Cell Receptor in Positive and Negative Selection of Developing T Cells
Blackman, M. A., Kappler, J. W., & Marrack, P. (1990) · Science · doi:10.1126/science.1972592 · Cited by 566
Seto, M., Jaeger, U., Hockett, R. D., Graninger, W. B., Bennett, S., Goldman, P., & Korsmeyer, S. J. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb02791.x · Cited by 484
Self-citationAntigen‐Driven Selection of Virgin and Memory B Cells
MacLennan, I. C. M., & Gray, D. (1986) · Immunological Reviews · doi:10.1111/j.1600-065x.1986.tb01484.x · Cited by 471
HL-60 cells induced to differentiate towards neutrophils subsequently die via apoptosis
Martin, S. J., BRADLEY, J. G., & Cotter, T. G. (1990) · Clinical & Experimental Immunology · doi:10.1111/j.1365-2249.1990.tb08110.x · Cited by 381
Chen-Levy, Z., Nourse, J. L., & Cleary, M. L. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.2.701 · Cited by 343
Cell death by apoptosis during involution of the lactating breast in mice and rats
Walker, N. I., Bennett, R. E., & Kerr, J. F. R. (1989) · American Journal of Anatomy · doi:10.1002/aja.1001850104 · Cited by 341
Silverberg, E., & Lubera, J. A. (1989) · CA A Cancer Journal for Clinicians · doi:10.3322/canjclin.39.1.3 · Cited by 313
Deletion of cells by apoptosis during castration-induced involution of the rat prostate
Kerr, J. F. R., & Searle, J. W. (1973) · Virchows Archiv B Cell Pathology Including Molecular Pathology · doi:10.1007/bf02889300 · Cited by 289
A Correlative Morphologic Study of Human Breast and Endometrium in the Menstrual Cycle
Longacre, T. A., & Bartow, S. A. (1986) · The American Journal of Surgical Pathology · doi:10.1097/00000478-198606000-00003 · Cited by 285
Expression of Bcl-2 and Bcl-2-Ig fusion transcripts in normal and neoplastic cells.
Graninger, W. B., Seto, M., Boutain, B., Goldman, P., & Korsmeyer, S. J. (1987) · Journal of Clinical Investigation · doi:10.1172/jci113235 · Cited by 280
Self-citationProgrammed death of autoreactive thymocytes
MacDonald, H. R., & Lees, R. K. (1990) · Nature · doi:10.1038/343642a0 · Cited by 224
McDonnell, T. J., Núñez, G., Platt, F. M., et al. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.5.1901 · Cited by 221
Self-citationNegrini, M., Silini, E. M., Kozak, C. A., Tsujimoto, Y., & Croce, C. M. (1987) · Cell · doi:10.1016/0092-8674(87)90448-x · Cited by 197
Prolonged antigen half-life in the lymphoid follicles of specifically immunized mice.
Tew, J. G., & MANDEL, T. E. (1979) · PubMed · Cited by 182
Hickey, W. F., Gonatas, N. K., Kimura, H., & Wilson, D. B. (1983) · The Journal of Immunology · doi:10.4049/jimmunol.131.6.2805 · Cited by 147
Self-citationUltrastructure of cell loss in intestinal mucosa
Potten, C. S., & Allen, T. D. (1977) · Journal of Ultrastructure Research · doi:10.1016/s0022-5320(77)80071-3 · Cited by 113
Mahmoud, I. J., Colin, I. M., Many, M., et al. (1986) · Experimental and Molecular Pathology · doi:10.1016/0014-4800(86)90040-7 · Cited by 94
Ultrastructural observations on cell death by apoptosis in the ?resting? human breast
Ferguson, D. J. P., & Anderson, T. J. (1981) · Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin · doi:10.1007/bf00431076 · Cited by 84
Smith, K. V. (1981) · Pathology · doi:10.1016/s0031-3025(16)37601-2 · Cited by 80
Nesidioblastosis and endocrine hyperplasia of the pancreas: A secondary phenomenon
Goudswaard, W. B., Houthoff, H. J., Koudstaal, J., & Zwierstra, R. P. (1986) · Human Pathology · doi:10.1016/s0046-8177(86)80154-x · Cited by 70
Chen-Levy, Z., Nourse, J. L., & Cleary, M. L. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.2.701-710.1989 · Cited by 62
Ultraviolet‐Induced Individual Cell Keratinization
Olson, R. L., Gaylor, J. A., & Everett, M. A. (1974) · Journal of Cutaneous Pathology · doi:10.1111/j.1600-0560.1974.tb00203.x · Cited by 42
The ultrastructure of the human epidermis in chronic graft-versus-host disease.
Gallucci, B. B., SHULMAN, H. M., Sale, G. E., et al. (1979) · PubMed · Cited by 42
Mcdonnell, T. J., Nunez, G., Platt, F. M., et al. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.5.1901-1907.1990 · Cited by 1
Cited by
Sorted by citations
Apoptosis in the Pathogenesis and Treatment of Disease
Thompson, C. B. (1995) · Science · doi:10.1126/science.7878464 · Cited by 6,524
Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death
Oltval, Z. N., Milliman, C. L., & Korsmeyer, S. J. (1993) · Cell · doi:10.1016/0092-8674(93)90509-o · Cited by 6,003
Self-citationBcl-2 functions in an antioxidant pathway to prevent apoptosis
Hockenbery, D. M., Oltvai, Z. N., Yin, X., Milliman, C. L., & Korsmeyer, S. J. (1993) · Cell · doi:10.1016/0092-8674(93)80066-n · Cited by 3,402
Self-citationA novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma
Ambrosini, G., Adida, C., & Altieri, D. C. (1997) · Nature Medicine · doi:10.1038/nm0897-917 · Cited by 3,174
Bcl-2 and the regulation of programmed cell death
Reed, J. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.124.1.1 · Cited by 2,287
Metadata
- Authors
- Hockenbery, D. M., Zutter, M., Hickey, W., Nahm, M., & Korsmeyer, S. J.
- Affiliations
- Washington University in St. Louis (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1991-08-15
- Volume
- 88
- Issue
- 16
- Pages
- 6961-6965
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC52213
- OpenAlex
- W1970629830
- Semantic Scholar
- d825dc162ad208c572711f59c9aa1c7974e89492
- Cited by
- 1,252
- References
- 40
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 9 · 2022: 4 · 2021: 5 · 2020: 10 · 2019: 9 · 2018: 9 · 2017: 9 · 2016: 7 · 2015: 10 · 2014: 15 · 2013: 10 · 2012: 12
- FWCI
- 26.75
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- Bcl-2, apoptosis, germinal center, light zone, plasma cells, memory B cells, thymic medulla, hematopoietic stem cells, tissue distribution, epithelial stem cells, follicular B-cell lymphoma, transgenic mice
- MeSH terms
- Adult, Bone Marrow, Breast, Cell Survival, Digestive System, Female, Humans, Immunoenzyme Techniques, Male, Neurons, Pancreas, Prostate, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogenes, Skin, Thymus Gland, Palatine Tonsil, Proto-Oncogene Proteins c-bcl-2
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