The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Gene inoculation generates immune responses against human immunodeficiency virus type 1.
Open accessAbstract
Recently, immunization techniques in which DNA constructs are introduced directly into mammalian tissue in vivo have been developed. In theory, gene inoculation should result in the production of antigenic proteins in a natural form in the immunized host. Here we present the use of such a technique for the inoculation of mice with a human immunodeficiency virus type 1 (HIV-1) envelope DNA construct (pM160). Mice were injected intramuscularly with pM160 and were subsequently analyzed for their anti-HIV envelope immune responses. Antisera collected from inoculated animals reacted with the recombinant HIV-1 envelope in ELISA and immunoprecipitation assays. The antisera also contained antibodies that were able to neutralize HIV-1 infection and inhibit HIV-1-mediated syncytium formation in vitro. Furthermore, splenic lymphocytes derived from pM160-inoculated animals demonstrated HIV-envelope-specific proliferative responses. The gene inoculation technique mimics features of vaccination with live attenuated viruses and, therefore, may ultimately prove useful in the rapid development of safe and efficacious vaccines as it provides for production of relevant antigen in vivo without the use of infectious agents.
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Cites
Direct Gene Transfer into Mouse Muscle in Vivo
Wolff, J. A., Malone, R. W., Williams, P., Chong, W., Acsadi, G., Jani, A., & Felgner, P. L. (1990) · Science · doi:10.1126/science.1690918 · Cited by 4,173
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Self-citationCancer Vaccines, Adjuvants, and Delivery Systems
Paston, S. J., Brentville, V. A., Symonds, P., & Durrant, L. G. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.627932 · Cited by 200
Self-replicating Semliki Forest virus RNA as recombinant vaccine
Zhou, X., Berglund, P., Rhodes, G., Parker, S. E., Jondal, M., & Liljeström, P. (1994) · Vaccine · doi:10.1016/0264-410x(94)90074-4 · Cited by 193
Polarization of immunity induced by direct injection of naked sequence-stabilized mRNA vaccines
Carralot, J.-P., Probst, J., Hoerr, I., Scheel, B., Teufel, R., Jung, G., Rammensee, H.-G., & Pascolo, S. (2004) · Cellular and Molecular Life Sciences CMLS · doi:10.1007/s00018-004-4255-0 · Cited by 149
Metadata
- Authors
- Wang, B., Ugen, K. E., Srikantan, V, Agadjanyan, M. G., Dang, K., Refaeli, Y., Sato, A. I., Boyer, J., Williams, W. V., & Weiner, D. B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1993-05
- Volume
- 90
- Issue
- 9
- Pages
- 4156-4160
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2009551908
- Cited by
- 655
- References
- 1
- FWCI
- 13.96
- Type
- journal article
- Language
- en
- Open access
- green
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- Wang, B., Ugen, K. E., Srikantan, V, Agadjanyan, M. G., Dang, K., Refaeli, Y., Sato, A. I., Boyer, J., Williams, W. V., & Weiner, D. B. (1993). Gene inoculation generates immune responses against human immunodeficiency virus type 1. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.90.9.4156
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