The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Purification of a Ran-interacting protein that is required for protein import into the nucleus.
Open accessAbstract
Previously we reported the isolation of two cytosolic fractions (A and B) from Xenopus ovary that are required sequentially to support protein import into the nuclei of digitonin-permeabilized cells. Fraction A is required for recognition of the nuclear localization sequence and targeting to the nuclear envelope, whereas fraction B is required for the subsequent translocation of the bound substrate into the nucleus. The first protein required for fraction B activity to be purified was the small GTPase Ran (ras-related nuclear protein). Here we report the purification of the second (and final) protein required for fraction B activity. By SDS/PAGE, the purified protein appeared as a single band with an apparent molecular mass of 10 kDa, but the native protein fractionated upon gel filtration chromatography with an apparent size of 30 kDa. Peptide sequence analysis indicated that the purified protein was highly homologous to a previously identified human protein of unknown function called placental protein 15 (pp15) and to the predicted protein product of a yeast open reading frame from Saccharomyces cerevisiae.
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Citations
Cites
The GTP-binding protein Ran/TC4 is required for protein import into the nucleus
Moore, M. S., & Blobel, G. (1993) · Nature · doi:10.1038/365661a0 · Cited by 759
Self-citationCatalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1
Bischoff, F. R., & Ponstingl, H. (1991) · Nature · doi:10.1038/354080a0 · Cited by 711
Melchior, F. (1993) · The Journal of Cell Biology · doi:10.1083/jcb.123.6.1649 · Cited by 544
Richardson, W. D., Mills, A. D., Dilworth, S. M., Laskey, R. A., & Dingwall, C. (1988) · Cell · doi:10.1016/0092-8674(88)90403-5 · Cited by 530
Nuclear import can be separated into distinct steps in vitro: Nuclear pore binding and translocation
Newmeyer, D. D., & Forbes, D. J. (1988) · Cell · doi:10.1016/0092-8674(88)90402-3 · Cited by 525
RanGAP1 induces GTPase activity of nuclear Ras-related Ran.
Bischoff, F. R., Klebe, C., Kretschmer, J., Wittinghofer, A., & Ponstingl, H. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.7.2587 · Cited by 523
Adam, E. J., & Adam, S. A. (1994) · The Journal of cell biology · doi:10.1083/jcb.125.3.547 · Cited by 304
Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide.
Bischoff, F. R., & Ponstingl, H. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.23.10830 · Cited by 277
Moore, M. S., & Blobel, G. (1992) · Cell · doi:10.1016/0092-8674(92)90613-h · Cited by 271
Self-citationPremature chromosome condensation in a ts DNA-mutant of BHK cells
Nishimoto, T., Eilen, E., & Basilico, C. (1978) · Cell · doi:10.1016/0092-8674(78)90017-x · Cited by 249
Cited by
Nucleocytoplasmic transport: the soluble phase
Mattaj, I. W., & Englmeier, L. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.265 · Cited by 1,175
Distinct functions for the two importin subunits in nuclear protein import
Görlich, D., Vogel, F., Mills, A. D., Hartmann, E., & Laskey, R. A. (1995) · Nature · doi:10.1038/377246a0 · Cited by 474
Fagotto, F., Glück, U., & Gumbiner, B. M. (1998) · Current Biology · doi:10.1016/s0960-9822(98)70082-x · Cited by 434
Köhler, M., Ansieau, S., Prehn, S., Leutz, A., Haller, H., & Hartmann, E. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)01265-9 · Cited by 228
Nuclear pore complexes — a doorway to neural injury in neurodegeneration
Coyne, A. N., & Rothstein, J. D. (2022) · Nature Reviews Neurology · doi:10.1038/s41582-022-00653-6 · Cited by 96
Metadata
- Authors
- Moore, M. S., & Blobel, G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1994-10-11
- Volume
- 91
- Issue
- 21
- Pages
- 10212-10216
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2071957053
- Cited by
- 320
- References
- 15
- FWCI
- 15.93
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
