Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 334

The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

Oxidation of cysteine-322 in the repeat domain of microtubule-associated protein tau controls the in vitro assembly of paired helical filaments.

Schweers, O, Mandelkow, E M, Biernat, J, Mandelkow, E·1995·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.92.18.8463
Open this paper in CoChat — free account

Abstract

One of the hallmarks of Alzheimer disease is the pathological aggregation of tau protein into paired helical filaments (PHFs) and neurofibrillary tangles. Here we describe the in vitro assembly of recombinant tau protein and constructs derived from it into PHFs. Though whole tau assembled poorly, constructs containing three internal repeats (corresponding to the fetal tau isoform) formed PHFs reproducibly. This ability depended on intermolecular disulfide bridges formed by the single Cys-322. Blocking the SH group, mutating Cys for Ala, or keeping tau in a reducing environment all inhibited assembly. With constructs derived from four-repeat tau (having the additional repeat no. 2 and a second Cys-291), PHF assembly was blocked because Cys-291 and Cys-322 interact within the molecule. PHF assembly was enabled again by mutating Cys-291 for Ala. The synthetic PHFs bound the dye thioflavin S used in Alzheimer disease diagnostics. The data imply that the redox potential in the neuron is crucial for PHF assembly, independently or in addition to pathological phosphorylation reactions.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1073/pnas.92.18.8463

Cites

4 of 4 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 4 references

Research works printed but not linked: 7

  1. [3]Kirschner, D. A., Abraham, C. & Selkoe, D. J. (1986) Proc. Natl. Acad. Sci. USA 83, 503–507.
  2. [13]Shearman, M. S., Ragan, C. I. & Iversen, L. L. (1994) Proc. Natl. Acad. Sci. USA 91, 1470–1474.
  3. [12]Smith, C. D., Carney, J. M., Starke-Reed, P. E., Oliver, C. N., Stadtman, E. R., Floyd, R. A. & Markesbery, W. R. (1991) Proc. Natl. Acad. Sci. USA 88, 10540–10543.
  4. [17]Greenberg, S. G. & Davies, P. (1990) Proc. Natl. Acad. Sci. USA 87, 5827-5831.
  5. [21]Wischik, C., Novak, M., Thogersen, H., Edwards, P., Runswick, M., Jakes, R., Walker, J., Milstein, C., Roth, M. & Klug, A. (1988) Proc. Natl. Acad. Sci. USA 85, 4506–4510.
  6. [32]Shigenaga, M. K., Hagen, T. M. & Ames, B. N. (1994) Proc. Natl. Acad. Sci. USA 91, 10771–10778.
  7. [35]Strittmatter, W. J., Weisgraber, K. H., Goedert, M., Saunders, A. M., Huang, D., Corder, E. H., Dong, L. M., Jakes, R., Alberts, M. J., Gilbert, J. R., Han, S. H., Hulette, C., Einstein, G., Schmechel, D., Pericak-Vance, M. & Roses, A. (1994) Exp. Neurol. 125, 163–171.

Cited by

Metadata

Authors
Schweers, O., Mandelkow, E. M., Biernat, J., & Mandelkow, E.
Affiliations
Max-Planck Unit for Structural Molecular Biology, c/o Deutsches Elektronen Synchroton, Hamburg, Germany.
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1995-08-29
Volume
92
Issue
18
Pages
8463-8467
ISSN
  • 0027-8424
  • 1091-6490
DOI
10.1073/pnas.92.18.8463
Cited by
334
References
4
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.