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Cited by 36Neuroscience and Neuropharmacology ResearchMemory and Neural MechanismsNeural dynamics and brain function

A presynaptic locus for long-term potentiation of elementary synaptic transmission at mossy fiber synapses in culture.

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López-García, J C, Arancio, O, Kandel, E R, Baranes, D·1996·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.93.10.4712PMC PMC39344
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Abstract

The complex circuitry of the CA3 region and the abundance of collateral connections has made it difficult to study the mossy fiber pathway in hippocampal slices and therefore to establish the site of expression of long-term potentiation at these synapses. Using a novel cell culture system, we have produced long-term potentiation of the elementary synaptic connections on single CA3 pyramidal neurons following tetanic stimulation of individual dentate gyrus granule cells. As is the case for the hippocampal slice, this potentiation was independent of N-methyl-D-aspartate receptor activation, was simulated by application of forskolin, and its induction did not require any modulatory input. The increase in synaptic strength was accompanied by a reduction in the number of failures of transmission and by an increase in the coefficient of variation of the responses and was prevented by presynaptic injection of an inhibitor of protein kinase A. These findings show that mossy fiber long-term potentiation has a presynaptic locus and that its expression is dependent on protein kinase A.

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Sorted by citations

Authors
López-García, J. C., Arancio, O., Kandel, E. R., & Baranes, D.
Affiliations
  • Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, NY 10032, USA.
  • Howard Hughes Medical Institute (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1996-05-14
Volume
93
Issue
10
Pages
4712-4717
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.93.10.4712
PMC
PMC39344
OpenAlex
W2030749284
Cited by
36
References
2
Citations by year
2024: 1 · 2023: 1 · 2021: 1 · 2016: 1 · 2014: 1 · 2012: 2
FWCI
0.81
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institute of General Medical Sciences (GM30299)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
Keywords
long-term potentiation, mossy fiber synapses, presynaptic plasticity, CA3 pyramidal cells, dentate gyrus granule cells, protein kinase A, forskolin, coefficient of variation, hippocampus
MeSH terms
Animals, Cells, Cultured, Dynorphins, Electrophysiology, Glutamine, Hippocampus, Synaptic Transmission, Synapses, Receptors, N-Methyl-D-Aspartate, Long-Term Potentiation, Pyramidal Cells, Dentate Gyrus, Rats

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