Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release
Open accessAbstract
Synaptophysin (syp I) is a synaptic vesicle membrane protein that constitutes approximately 7% of the total vesicle protein. Multiple lines of evidence implicate syp I in a number of nerve terminal functions. To test these, we have disrupted the murine Syp I gene. Mutant mice lacking syp I were viable and fertile. No changes in the structure and protein composition of the mutant brains were observed except for a decrease in synaptobrevin/VAMP II. Synaptic transmission was normal with no detectable changes in synaptic plasticity or the probability of release. Our data demonstrate that one of the major synaptic vesicle membrane proteins is not essential for synaptic transmission, suggesting that its function is either redundant or that it has a more subtle function not apparent in the assays used.
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The potency (average amplitude of EPSC successes) was about two-fold larger at CA2-CA1 synapses, around −8 pA, than previously reported for CA3-CA1 synapses, around −4 pA.
“However, the potency (average amplitude of EPSC successes) was about two-fold larger at CA2–CA1 synapses, around −8 pA (Fig. 8D), than previously reported for CA3–CA1 synapses, around−4 pA (see (Bolshakov and Siegelbaum, 1995; McMahon et al., 1996).”
From Strong CA2 Pyramidal Neuron Synapses Define a Powerful Disynaptic Cortico-Hippocampal Loop (2010) · cites this paper as McMahon et al., 1996
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- Authors
- McMahon, H. T., Bolshakov, V. Y., Janz, R., Hammer, R. E., Siegelbaum, S. A., & Südhof, T. C.
- Affiliations
- Howard Hughes Medical Institute (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1996-05-14
- Volume
- 93
- Issue
- 10
- Pages
- 4760-4764
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC39352
- OpenAlex
- W2163967516
- Cited by
- 262
- References
- 32
- Citations by year
- 2026: 6 · 2025: 3 · 2024: 5 · 2023: 6 · 2022: 9 · 2021: 13 · 2020: 9 · 2019: 8 · 2018: 10 · 2017: 13 · 2016: 11 · 2015: 5 · 2014: 9 · 2013: 6 · 2012: 10
- FWCI
- 3.81
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- synaptophysin, neurotransmitter release, synaptic transmission, knockout mice, synaptic plasticity, release probability, functional redundancy
- MeSH terms
- Animals, Base Sequence, Calcium, Chromosome Mapping, Electrophysiology, Humans, Molecular Sequence Data, Synaptic Transmission, Neuronal Plasticity, Phenotype, Synaptic Vesicles, Synaptophysin, DNA Primers, Mice, Knockout, Neurotransmitter Agents, Mice
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