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A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae : YCF1-catalyzed transport of bis(glutathionato)cadmium
Open accessAbstract
The yeast cadmium factor ( YCF1 ) gene encodes an MgATP-energized glutathione S -conjugate transporter responsible for the vacuolar sequestration of organic compounds after their S -conjugation with glutathione. However, while YCF1 was originally isolated according to its ability to confer resistance to cadmium salts, neither its mode of interaction with Cd 2+ nor the relationship between this process and organic glutathione-conjugate transport are known. Here we show through direct comparisons between vacuolar membrane vesicles purified from Saccharomyces cerevisiae strain DTY167, harboring a deletion of the YCF1 gene, and the isogenic wild-type strain DTY165 that YCF1 mediates the MgATP-energized vacuolar accumulation of Cd·glutathione complexes. The substrate requirements, kinetics and Cd 2+ /glutathione stoichiometry of cadmium uptake and the molecular weight of the transport-active complex demonstrate that YCF1 selectively catalyzes the transport of bis(glutathionato)cadmium (Cd·GS 2 ). On the basis of these results—the Cd 2+ hypersensitivity of DTY167, versus DTY165, cells, the inducibility of YCF1-mediated transport, and the rapidity and spontaneity of Cd·GS 2 formation—this new pathway is concluded to contribute substantially to Cd 2+ detoxification.
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Cites
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The OsNRAMP1 iron transporter is involved in Cd accumulation in rice
Takahashi, R., Ishimaru, Y., Senoura, T., Shimo, H., Ishikawa, S., Arao, T., Nakanishi, H., & Nishizawa, N. K. (2011) · Journal of Experimental Botany · doi:10.1093/jxb/err136 · Cited by 591
Metadata
- Authors
- Li, Z.-S., Lu, Y.-P., Zhen, R.-G., Szczypka, M., Thiele, D. J., & Rea, P. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-01-07
- Volume
- 94
- Issue
- 1
- Pages
- 42-47
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2057865646
- Cited by
- 607
- References
- 2
- FWCI
- 18.30
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae : YCF1-catalyzed transport of bis(glutathionato)cadmium. https://app.cochat.ai/papers/10.1073/pnas.94.1.42
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- Li, Z.-S., Lu, Y.-P., Zhen, R.-G., Szczypka, M., Thiele, D. J., & Rea, P. A. (1997). A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae : YCF1-catalyzed transport of bis(glutathionato)cadmium. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.1.42
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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