The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Quantitative neuropathology by high resolution magic angle spinning proton magnetic resonance spectroscopy
Open accessAbstract
We describe a method that directly relates tissue neuropathological analysis to medical imaging. Presently, only indirect and often tenuous relationships are made between imaging (such as MRI or x-ray computed tomography) and neuropathology. We present a biochemistry-based, quantitative neuropathological method that can help to precisely quantify information provided by in vivo proton magnetic resonance spectroscopy ( 1 HMRS), an emerging medical imaging technique. This method, high resolution magic angle spinning (HRMAS) 1 HMRS, is rapid and requires only small amounts of unprocessed samples. Unlike chemical extraction or other forms of tissue processing, this method analyzes tissue directly, thus minimizing artifacts. We demonstrate the utility of this method by assessing neuronal damage using multiple tissue samples from differently affected brain regions in a case of Pick disease, a human neurodegenerative disorder. Among different regions, we found an excellent correlation between neuronal loss shown by traditional neurohistopathology and decrease of the neuronal marker N -acetylaspartate measured by HRMAS 1 HMRS. This result demonstrates for the first time, to our knowledge, a direct, quantitative link between a decrease in N -acetylaspartate and neuronal loss in a human neurodegenerative disease. As a quantitative method, HRMAS 1 HMRS has potential applications in experimental and clinical neuropathologic investigations. It should also provide a rational basis for the interpretation of in vivo 1 HMRS studies of human neurological disorders.
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N-ACETYL-l-ASPARTIC ACID IN BRAIN
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Enhanced resolution of proton NMR spectra of malignant lymph nodes using magic‐angle spinning
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Cited by
Atherton, H. J., Bailey, N. J., Zhang, W., Taylor, J., Major, H., Shockcor, J., Clarke, K., & Griffin, J. L. (2006) · Physiological Genomics · doi:10.1152/physiolgenomics.00060.2006 · Cited by 159
Sitter, B., Bathen, T. F., Tessem, M.-B., & Gribbestad, I. S. (2009) · Progress in Nuclear Magnetic Resonance Spectroscopy · doi:10.1016/j.pnmrs.2008.10.001 · Cited by 91
Cheng, L. L., Newell, K., Mallory, A. E., Hyman, B. T., & Gonzalez, R. G. (2002) · Magnetic Resonance Imaging · doi:10.1016/s0730-725x(02)00512-x · Cited by 77
Self-citationHR-MAS NMR Based Quantitative Metabolomics in Breast Cancer
Gogiashvili, M., Nowacki, J., Hergenröder, R., Hengstler, J. G., Lambert, J., & Edlund, K. (2019) · Metabolites · doi:10.3390/metabo9020019 · Cited by 44
Metadata
- Authors
- Cheng, L. L., Ma, M. J., Becerra, L., Ptak, T., Tracey, I., Lackner, A., & González, R. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-06-10
- Volume
- 94
- Issue
- 12
- Pages
- 6408-6413
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2121817566
- Cited by
- 339
- References
- 23
- FWCI
- 10.58
- Type
- journal article
- Language
- en
- Open access
- green
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- Cheng, L. L., Ma, M. J., Becerra, L., Ptak, T., Tracey, I., Lackner, A., & González, R. G. (1997). Quantitative neuropathology by high resolution magic angle spinning proton magnetic resonance spectroscopy. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.12.6408
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