The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A Conserved Binding Motif Defines Numerous Candidate Target Proteins for Both Cdc42 and Rac GTPases
Open accessAbstract
Rho, Rac, and Cdc42 are small GTPases that regulate the formation of a variety of actin structures and the assembly of associated integrin complexes, but little is known about the target proteins that mediate their effects. Here we have used a motif-based search method to identify putative effector proteins for Rac and Cdc42. A search of the GenBankTM data base for similarity with the minimum Cdc42/Rac interactive binding (CRIB) region of a potential effector protein p65PAK has identified over 25 proteins containing a similar motif from a range of different species. These candidate Cdc42/Rac-binding proteins include family members of the mixed lineage kinases (MLK), a novel tyrosine kinase from Drosophila melanogaster (DPR2), a human protein MSE55, and several novel yeast and Caenorhabditis elegans proteins. Two murine p65PAK isoforms and a candidate protein from C. elegans, F09F7.5, interact strongly with the GTP form of both Cdc42 and Rac, but not Rho in a filter binding assay. Three additional candidate proteins, DPR2, MSE55, and MLK3 showed binding to the GTP form of Cdc42 and weaker binding with Rac, and again no interaction with Rho. These results indicate that proteins containing the CRIB motif bind to Cdc42 and/or Rac in a GTP-dependent manner, and they may, therefore, participate in downstream signaling.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Basic local alignment search tool
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990) · Journal of Molecular Biology · doi:10.1006/jmbi.1990.9999 · Cited by 98,669
Cited by
RHO GTPASES: Biochemistry and Biology
Jaffe, A. B., & Hall, A. (2005) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.21.020604.150721 · Cited by 3,009
Self-citationRho GTPases and their effector proteins
BISHOP, A. L., & HALL, A. (2000) · Biochemical Journal · doi:10.1042/bj3480241 · Cited by 2,575
Self-citationThe cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42
Joberty, G., Petersen, C., Gao, L., & Macara, I. G. (2000) · Nature Cell Biology · doi:10.1038/35019573 · Cited by 928
Watanabe, N., Madaule, P., Reid, T., Ishizaki, T., Watanabe, G., Kakizuka, A., Saito, Y., Nakao, K., Jockusch, B. M., & Narumiya, S. (1997) · The EMBO Journal · doi:10.1093/emboj/16.11.3044 · Cited by 823
A mammalian PAR-3–PAR-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity
Lin, D., Edwards, A. S., Fawcett, J. P., Mbamalu, G., Scott, J. D., & Pawson, T. (2000) · Nature Cell Biology · doi:10.1038/35019582 · Cited by 743
Benard, V., Bohl, B. P., & Bokoch, G. M. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.19.13198 · Cited by 645
Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells
Sells, M. A., Knaus, U. G., Bagrodia, S., Ambrose, D. M., Bokoch, G. M., & Chernoff, J. (1997) · Current Biology · doi:10.1016/s0960-9822(97)70091-5 · Cited by 603
Microtubule growth activates Rac1 to promote lamellipodial protrusion in fibroblasts
Waterman-Storer, C. M., Worthylake, R. A., Liu, B. P., Burridge, K., & Salmon, E. D. (1999) · Nature Cell Biology · doi:10.1038/9018 · Cited by 491
Macara, I. G. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1332 · Cited by 341
Structure of the TPR Domain of p67phox in Complex with Rac·GTP
Lapouge, K., Smith, S. J. M., Walker, P. A., Gamblin, S. J., Smerdon, S. J., & Rittinger, K. (2000) · Molecular Cell · doi:10.1016/s1097-2765(05)00091-2 · Cited by 311
p140Sra-1 (specifically Rac1-associated protein) is a novel specific target for Rac1 small GTPase
Kobayashi, K., Kuroda, S., Fukata, M., Nakamura, T., Nagase, T., Nomura, N., Matsuura, Y., Yoshida-Kubomura, N., Iwamatsu, A., & Kaibuchi, K. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.1.291 · Cited by 244
Koga, H., Terasawa, H., Nunoi, H., Takeshige, K., Inagaki, F., & Sumimoto, H. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.35.25051 · Cited by 224
Leng, Y., Zhang, J., Badour, K., Arpaia, E., Freeman, S., Cheung, P., Siu, M., & Siminovitch, K. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0409120102 · Cited by 210
Buchsbaum, R. J., Connolly, B. A., & Feig, L. A. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.12.4073-4085.2002 · Cited by 198
Filopodia are conduits for melanosome transfer to keratinocytes
Scott, G., Leopardi, S., Printup, S., & Madden, B. C. (2002) · Journal of Cell Science · doi:10.1242/jcs.115.7.1441 · Cited by 150
Structural requirements for PAK activation by Rac GTPases
Knaus, U. G., Wang, Y., Reilly, A. M., Warnock, D., & Jackson, J. H. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.34.21512 · Cited by 111
A Drosophila MAPKKK, D‐MEKK1, mediates stress responses through activation of p38 MAPK
Inoue, H., Tateno, M., Fujimura‐Kamada, K., Takaesu, G., Adachi‐Yamada, T., Ninomiya‐Tsuji, J., Irie, K., Nishida, Y., & Matsumoto, K. (2001) · The EMBO Journal · doi:10.1093/emboj/20.19.5421 · Cited by 99
Fam49/CYRI interacts with Rac1 and locally suppresses protrusions
Fort, L., Batista, J. M., Thomason, P. A., Spence, H. J., Whitelaw, J. A., Tweedy, L., Greaves, J., Martin, K. J., Anderson, K. I., Brown, P., et al. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0198-9 · Cited by 98
Jeong, J.-W., Lee, H. S., Lee, K. Y., White, L. D., Broaddus, R. R., Zhang, Y.-W., Vande Woude, G. F., Giudice, L. C., Young, S. L., Lessey, B. A., et al. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0903632106 · Cited by 92
Carstanjen, D., Yamauchi, A., Koornneef, A., Zang, H., Filippi, M.-D., Harris, C., Towe, J., Atkinson, S., Zheng, Y., Dinauer, M. C., et al. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.174.8.4613 · Cited by 56
Jiang, B., Zhang, J., Xia, J., Zhao, W., Wu, Y., Shi, M., Luo, L., Zhou, H., Chen, A., Ma, H., et al. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.03.053 · Cited by 33
Ruan, J., Lai, L., Ou, H., & Yi, P. (2023) · Nature Communications · doi:10.1038/s41467-023-42879-y · Cited by 23
Metadata
- Authors
- Burbelo, P. D., Drechsel, D., & Hall, A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-12
- Volume
- 270
- Issue
- 49
- Pages
- 29071-29074
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2036123452
- Cited by
- 622
- References
- 1
- FWCI
- 14.33
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
