Cited by 161Alzheimer's disease research and treatmentsNeuroinflammation and Neurodegeneration MechanismsDementia and Cognitive Impairment Research

Amyloids and Are Generated Intracellularly in Cultured Human Neurons and Their Secretion Increases with Maturation

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Turner, R. Scott, Suzuki, Nobuhiro, Chyung, Abraham S.C., Younkin, Steven G., Lee, Virginia M.-Y.·1996·Journal of Biological Chemistry

Abstract

Previous studies have demonstrated the presence of amyloid beta (Abeta) in neurons (NT2N) derived from a human embryonal carcinoma cell line (NT2) by steady state metabolic radiolabeling and immunoprecipitation. We show here that Abeta is present intracellularly since trypsin digestion of intact NT2N cells at 4 degrees C did not eliminate the Abeta recovered in cell lysates. To determine whether both Abeta40 and Abeta42 are produced intracellularly, quantitative sandwich enzyme-linked immunosorbent assay (ELISA) was performed using COOH-terminal end-specific anti-Abeta monoclonal antibodies. Sandwich ELISA detected intracellular Abeta40 and A++beta42 in NT2N cell lysates at a ratio of 3:1, whereas secreted Abeta40 and Abeta42 were recovered in medium conditioned by NT2N cells at a ratio of approximately 20:1. Metabolic steady state and pulse-chase labeling studies demonstrated a 2-h delay in the detection of cell-associated Abeta40/Abeta42 in the medium, suggesting that Abeta is generated at a slow rate intracellularly prior to its secretion. Finally, as NT2N cells mature over time in culture, the secretion of Abeta40 and Abeta42 increases more than 5-fold over 7 weeks. This increase in the secretion of Abeta40/Abeta42 in NT2N cells as a function of time may recapitulate a similar phenomenon in the aging brain.

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Crossref · 10.1074/jbc.271.15.8966

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  • Subsequent work showed that particularly the disease-linked Aβ42 peptide, rather than the more abundantly secreted Aβ40 peptide, increased with differentiation and time in culture within clonal NT2 cells.

    “Subsequent work showed that particularly the disease-linked Aβ42 peptide, rather than the more abundantly secreted Aβ40 peptide, increased with differentiation and time in culture within clonal NT2 cells that can develop a neuron-like phenotype [174, 195].”

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Authors
Turner, R. S., Suzuki, N., Chyung, A. S. C., Younkin, S. G., & Lee, V. M.-Y.
Affiliations
  • University of Pennsylvania (US)
  • Case Western Reserve University (US)
Corresponding author
Lee, Virginia M.-Y.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1996-04
Volume
271
Issue
15
Pages
8966-8970
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1998238542
Cited by
161
References
35
Citations by year
2025: 1 · 2024: 1 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 6
FWCI
9.44
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
National Institute on Aging (AG09215, AG06656, P01AG009215, R01AG006656)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
Keywords
amyloid-beta, intracellular amyloid-beta, Aβ40, amyloid-beta 42, human neurons, neuronal maturation, pulse-chase labeling, trypsin digestion, brain aging, Alzheimer's disease
MeSH terms
Cells, Cultured, Cytoplasm, Humans, Molecular Weight, Nerve Tissue Proteins, Neurons, Peptide Fragments, Amyloid beta-Peptides, Cellular Senescence

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