Cited by 740Alzheimer's disease research and treatmentsDementia and Cognitive Impairment ResearchIntracerebral and Subarachnoid Hemorrhage Research

beta-Amyloid-(1-42) is a major component of cerebrovascular amyloid deposits: implications for the pathology of Alzheimer disease.

Open access
Roher, A E, Lowenson, J D, Clarke, S, Woods, A S, Cotter, R J, Gowing, E, Ball, M J·1993·Proceedings of the National Academy of Sciences

Abstract

Reinvestigation of the chemical structure of beta-amyloid peptide (A beta) deposits in the vascular tissue of Alzheimer disease brains revealed that the 42-residue form A beta-(1-42), rather than the more soluble A beta-(1-40) form, is the predominant peptide. Following removal of the surrounding tissue with SDS and collagenase, A beta was solubilized in formic acid and purified by Superose 12 chromatography. Peptides generated by enzymatic and chemical digestion of the A beta were purified by HPLC and characterized by amino acid analysis, sequence analysis, and mass spectrometry. In the leptomeningeal vessels, the average ratio of A beta-(1-42)/A beta-(1-40) was 58:42, whereas in the parenchymal vessels this ratio was 75:25. Interestingly, vascular A beta contains considerably less isomerized and racemized aspartyl residues than does neuritic plaque A beta, suggesting that the vascular amyloid is "younger." The discrete nature of the bands and spherical deposits of A beta associated with arterioles and capillaries, respectively, suggests that this amyloid arises from the vascular tissue itself. Increasing A beta deposition appears to lead to the distortion and occlusion of capillaries, which may contribute significantly to the pathology of Alzheimer disease.

Integrity signals

Registry

Crossref · 10.1073/pnas.90.22.10836

Cites

19 of 19 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Roher, A. E., Lowenson, J. D., Clarke, S., Woods, A. S., Cotter, R. J., Gowing, E., & Ball, M. J.
Affiliations
Wayne State University (US)
Venue
Proceedings of the National Academy of Sciences
Published
1993-11-15
Volume
90
Issue
22
Pages
10836-10840
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2081182167
Cited by
740
References
19
Citations by year
2026: 13 · 2025: 21 · 2024: 26 · 2023: 14 · 2022: 45 · 2021: 20 · 2020: 23 · 2019: 21 · 2018: 23 · 2017: 20 · 2016: 24 · 2015: 28 · 2014: 24 · 2013: 28 · 2012: 17
FWCI
16.73
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Science Foundation
  • American Health Assistance Foundation
  • American Heart Association
  • National Institutes of Health (AG‐08017)
  • National Institute on Aging (P30AG008017)
  • National Institute of General Medical Sciences (GM 26020)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
  • Health Sciences › Medicine › Neurology › Intracerebral and Subarachnoid Hemorrhage Research
Keywords
amyloid-beta, Alzheimer disease pathology, leptomeningeal vessels, arterioles, capillaries, capillary occlusion
MeSH terms
Alzheimer Disease, Amino Acid Sequence, Blood-Brain Barrier, Brain Chemistry, Cerebrovascular Circulation, Humans, Microscopy, Fluorescence, Molecular Sequence Data, Oligopeptides, Mass Spectrometry, Amyloid beta-Peptides

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review