Cited by 9Plant Virus Research StudiesPlant Molecular Biology ResearchEpigenetics and DNA Methylation

Sequence-specific and Methylation-dependent and -independent Binding of Rice Nuclear Proteins to a Rice Tungro Bacilliform Virus Vascular Bundle Expression Element

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He, Xiaoyuan, Fütterer, Johannes, Hohn, Thomas·2001·Journal of Biological Chemistry

Abstract

Nuclear proteins from rice (Oryza sativa) were identified that bind specifically to a rice tungro bacilliform virus promoter region containing a vascular bundle expression element (VBE). One set of proteins of 29, 33, and 37 kDa, present in shoot and cell suspension extracts but hardly detectable in root extracts, bound to a site containing the sequence AGAAGGACCAGA within the VBE, which also contains two CpG and one CpNpG potential methylation motifs. Binding by these proteins was determined to be cytosine methylation-independent. However, a novel protein present in all analyzed extracts bound specifically to the methylated VBE. A region of at least 49 nucleotides overlapping the VBE and complete cytosine methylation of the three Cp(Np)G motifs was required for efficient binding of this methylated VBE-binding protein (MVBP).

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Crossref · 10.1074/jbc.m006653200

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  • The rice nuclear protein MVBP binds a rice tungro bacilliform virus promoter region only when the promoter is methylated.

    “the nuclear protein MVBP (methylated VBE-binding protein) was shown to bind to a rice tungro bacilliform virus promoter region only when the promoter was methylated46”

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Authors
He, X., Fütterer, J., & Hohn, T.
Affiliations
Friedrich Miescher Institute (CH)
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2001-01
Volume
276
Issue
4
Pages
2644-2651
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2163361806
Cited by
9
References
54
Citations by year
2024: 1 · 2016: 1
FWCI
0.91
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Virus Research Studies
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Keywords
rice tungro bacilliform virus, nuclear proteins, protein-DNA interactions, cytosine methylation, CpG motifs, promoter, rice
MeSH terms
Base Sequence, Cytosine, DNA-Binding Proteins, Molecular Sequence Data, Nuclear Proteins, Plant Proteins, Promoter Regions, Genetic, Protein Binding, Oryza, Tissue Distribution, Plant Shoots, CpG Islands, Badnavirus, 5-Methylcytosine

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