Sequence-specific and Methylation-dependent and -independent Binding of Rice Nuclear Proteins to a Rice Tungro Bacilliform Virus Vascular Bundle Expression Element
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Nuclear proteins from rice (Oryza sativa) were identified that bind specifically to a rice tungro bacilliform virus promoter region containing a vascular bundle expression element (VBE). One set of proteins of 29, 33, and 37 kDa, present in shoot and cell suspension extracts but hardly detectable in root extracts, bound to a site containing the sequence AGAAGGACCAGA within the VBE, which also contains two CpG and one CpNpG potential methylation motifs. Binding by these proteins was determined to be cytosine methylation-independent. However, a novel protein present in all analyzed extracts bound specifically to the methylated VBE. A region of at least 49 nucleotides overlapping the VBE and complete cytosine methylation of the three Cp(Np)G motifs was required for efficient binding of this methylated VBE-binding protein (MVBP).
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The rice nuclear protein MVBP binds a rice tungro bacilliform virus promoter region only when the promoter is methylated.
“the nuclear protein MVBP (methylated VBE-binding protein) was shown to bind to a rice tungro bacilliform virus promoter region only when the promoter was methylated46”
From Transcription factors as readers and effectors of DNA methylation (2016) · cites this paper as [46]
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- Authors
- He, X., Fütterer, J., & Hohn, T.
- Affiliations
- Friedrich Miescher Institute (CH)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-01
- Volume
- 276
- Issue
- 4
- Pages
- 2644-2651
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2163361806
- Cited by
- 9
- References
- 54
- Citations by year
- 2024: 1 · 2016: 1
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Virus Research Studies
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- rice tungro bacilliform virus, nuclear proteins, protein-DNA interactions, cytosine methylation, CpG motifs, promoter, rice
- MeSH terms
- Base Sequence, Cytosine, DNA-Binding Proteins, Molecular Sequence Data, Nuclear Proteins, Plant Proteins, Promoter Regions, Genetic, Protein Binding, Oryza, Tissue Distribution, Plant Shoots, CpG Islands, Badnavirus, 5-Methylcytosine
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