CpG methylation inhibits binding of several sequence-specific DNA-binding proteins from pea, wheat, soybean and cauliflower

Inamdar, Nilufar M.; Ehrlich, Kenneth C.; Ehrlich, Melanie

doi:10.1007/bf00036811

Cited by 120Epigenetics and DNA MethylationPlant Molecular Biology ResearchGenomics and Chromatin Dynamics

CpG methylation inhibits binding of several sequence-specific DNA-binding proteins from pea, wheat, soybean and cauliflower

Inamdar, Nilufar M., Ehrlich, Kenneth C., Ehrlich, Melanie·1991·Plant Molecular Biology

DOI 10.1007/bf00036811

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Abstract

To elucidate how methylation of specific sites in plant DNA might control transcription, we examined the effect of DNA methylation at CpG sequences on the binding of plant nuclear factors to an oligonucleotide duplex containing the consensus sequence for mammalian CREB (cAMP response element binding protein). CREB is part of the ATF (activating transcription factor) family of mammalian proteins specifically binding to 5'-TGACGTCA-3' and related sequences. Proteins recognizing the CREB-specific ligand were identified in nuclear extracts of pea seeds, wheat germ, cauliflower, and soybean leaves using electrophoretic mobility shift assays. Cytosine methylation inhibited binding of this protein in all these extracts, and so this sequence-specific DNA-binding activity is referred to as methylation-inhibited binding protein 1 (MIB-1). Sites somewhat similar to that of the CREB ligand are found in the upstream regions of a wheat histone H3 gene and tomato and pea ribulose 1,5-bisphosphate carboxylase genes. These sites were bound preferentially by distinct proteins that may be related to the previously described plant proteins HBP-1, HSBF, ASF-1, or GBF. Methylation of cytosine residues at these sites and at a site for MIB-1 located upstream of a soybean proline-rich protein gene also reduced specific binding with all the nuclear extracts tested. Similarly, substitution of the central CpG dinucleotide with TpG decreased binding.

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Manipulation of the methylation status of DNA sequences results in differential binding of transcription factors, including E2F, AP2α, MYC and MYN.
“The manipulation of the methylation status of DNA sequences has been shown (mostly in in vitro studies) to result in the differential binding of TFs, including E2F, AP2α, MYC and MYN88–95.”
From Transcription factors as readers and effectors of DNA methylation (2016) · cites this paper as [93]

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Authors: Inamdar, N. M., Ehrlich, K. C., & Ehrlich, M.
Affiliations: Tulane University (US)
Southern Regional Research Center (US)
ORCID: Ehrlich, Kenneth C.
Ehrlich, Melanie
Venue: Plant Molecular Biology
Publisher: Springer Science and Business Media LLC
Published: 1991-07
Volume: 17
Issue: 1
Pages: 111-123
ISSN: 0167-4412 (ISSN-L)
1573-5028
DOI: 10.1007/bf00036811
OpenAlex: W1987717753
Cited by: 120
References: 45
Citations by year: 2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 3 · 2016: 8 · 2015: 4 · 2014: 5 · 2013: 3 · 2012: 10
FWCI: 1.72
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Plant Molecular Biology (publishedVersion)
PubMed (publishedVersion)
Funders: National Science Foundation
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords: DNA methylation, CpG methylation, CREB-binding protein, sequence-specific DNA-binding proteins, transcriptional regulation, pea, wheat, soybean, cauliflower, histone H3 gene, Rubisco
MeSH terms: Base Sequence, Binding Sites, Brassica, DNA, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Histones, Fabaceae, Methylation, Molecular Sequence Data, Oligonucleotides, Plant Proteins, Plants, Plants, Medicinal, Promoter Regions, Genetic, Glycine max, Triticum, Dinucleoside Phosphates, Consensus Sequence, Cyclic AMP Response Element-Binding Protein

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CpG methylation inhibits binding of several sequence-specific DNA-binding proteins from pea, wheat, soybean and cauliflower

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