Sema4C, a Transmembrane Semaphorin, Interacts with a Post-synaptic Density Protein, PSD-95
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Semaphorins are known to act as chemorepulsive molecules that guide axons during neural development. Sema4C, a group 4 semaphorin, is a transmembrane semaphorin of unknown function. The cytoplasmic domain of Sema4C contains a proline-rich region that may interact with some signaling proteins. In this study, we demonstrate that Sema4C is enriched in the adult mouse brain and associated with PSD-95 isoforms containing PDZ (PSD-95/DLG/ZO-1) domains, such as PSD-95/SAP90, PSD-93/chapsin110, and SAP97/DLG-1, which are concentrated in the post-synaptic density of the brain. In the neocortex, S4C is enriched in the synaptic vesicle fraction and Triton X-100 insoluble post-synaptic density fraction. Immunostaining for Sema4C overlaps that for PSD-95 in superficial layers I-IV of the neocortex. In neocortical culture, S4C is colocalized with PSD-95 in neurons, with a dot-like pattern along the neurites. Sema4C thus may function in the cortical neurons as a bi-directional transmembrane ligand through interacting with PSD-95.
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Sema4c is known as interaction partner of different PSD95 isoforms in neocortical cultures and in the adult brain of mice.
“For example, in the quadruple knockout mouse Sema4c is upregulated, which is known as interaction partner of different PSD95 isoforms in neocortical cultures as well as in the adult brain of mice63.”
From Elimination of the four extracellular matrix molecules tenascin-C, tenascin-R, brevican and neurocan alters the ratio of excitatory and inhibitory synapses (2019) · cites this paper as [63]
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Toguchi, M., Gonzalez, D., Furukawa, S., & Inagaki, S. (2009) · Neurochemistry International · doi:10.1016/j.neuint.2009.05.013 · Cited by 32 · see citing works
Self-citationGiacobini, P. (2015) · Neuroendocrinology · doi:10.1159/000431021 · Cited by 30 · see citing works
Requirement of the transmembrane semaphorin Sema4C for myogenic differentiation
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Self-citationThe resilient synapse: insights from genetic interference of synaptic cell adhesion molecules
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SEMA6B variants cause intellectual disability and alter dendritic spine density and axon guidance
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Semaphorin 4C: A Novel Component of B-Cell Polarization in Th2-Driven Immune Responses
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Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1.
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New Promising Routes in Peptic Ulcers: Toll-like Receptors and Semaphorins
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Sema4C Is Required for Vascular and Primary Motor Neuronal Patterning in Zebrafish
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Structural and functional characterization of interactions of myotubularin-related proteins
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Shedding light on the proteolytically released ectodomains of membrane-bound Semaphorins
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- Authors
- Inagaki, S., Ohoka, Y., Sugimoto, H., Fujioka, S., Amazaki, M., Kurinami, H., Miyazaki, N., Tohyama, M., & Furuyama, T.
- Affiliations
- The University of Osaka (JP)
- Corresponding author
- Inagaki, Shinobu
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-03
- Volume
- 276
- Issue
- 12
- Pages
- 9174-9181
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2047120515
- Cited by
- 63
- References
- 52
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 3 · 2016: 6 · 2015: 3 · 2014: 3 · 2013: 1 · 2012: 3
- FWCI
- 1.22
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Keywords
- Sema4C, semaphorins, PSD-95, postsynaptic density, PDZ domain, synaptic vesicles, neocortex, cortical neurons, axon guidance, SAP97
- MeSH terms
- Disks Large Homolog 4 Protein, Animals, Base Sequence, Binding Sites, Cerebral Cortex, Membrane Proteins, Nerve Tissue Proteins, Protein Binding, Subcellular Fractions, DNA Primers, Gene Expression Regulation, Developmental, Two-Hybrid System Techniques, Semaphorins, Intracellular Signaling Peptides and Proteins, Mice, Rats, Guanylate Kinases
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