Cited by 63Axon Guidance and Neuronal SignalingNeuroscience and Neuropharmacology ResearchNerve injury and regeneration

Sema4C, a Transmembrane Semaphorin, Interacts with a Post-synaptic Density Protein, PSD-95

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Inagaki, Shinobu, Ohoka, Yoshiharu, Sugimoto, Hisako, Fujioka, Shiho, Amazaki, Mayumi, Kurinami, Hitomi, Miyazaki, Nobuo, Tohyama, Masaya +1 more·2001·Journal of Biological Chemistry

Abstract

Semaphorins are known to act as chemorepulsive molecules that guide axons during neural development. Sema4C, a group 4 semaphorin, is a transmembrane semaphorin of unknown function. The cytoplasmic domain of Sema4C contains a proline-rich region that may interact with some signaling proteins. In this study, we demonstrate that Sema4C is enriched in the adult mouse brain and associated with PSD-95 isoforms containing PDZ (PSD-95/DLG/ZO-1) domains, such as PSD-95/SAP90, PSD-93/chapsin110, and SAP97/DLG-1, which are concentrated in the post-synaptic density of the brain. In the neocortex, S4C is enriched in the synaptic vesicle fraction and Triton X-100 insoluble post-synaptic density fraction. Immunostaining for Sema4C overlaps that for PSD-95 in superficial layers I-IV of the neocortex. In neocortical culture, S4C is colocalized with PSD-95 in neurons, with a dot-like pattern along the neurites. Sema4C thus may function in the cortical neurons as a bi-directional transmembrane ligand through interacting with PSD-95.

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  1. Goslin, K., and Banker, G. (1991) Culturing Nerve Cells (Goslin, K., and Banker, G., eds) MIT Press, Cambridge, MA, pp. 339 -370
  2. Lue, R. A., Marfatia, S. M., Branton, D., and Chishti, A. H. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 9818 -9822
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Authors
Inagaki, S., Ohoka, Y., Sugimoto, H., Fujioka, S., Amazaki, M., Kurinami, H., Miyazaki, N., Tohyama, M., & Furuyama, T.
Affiliations
The University of Osaka (JP)
Corresponding author
Inagaki, Shinobu
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2001-03
Volume
276
Issue
12
Pages
9174-9181
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2047120515
Cited by
63
References
52
Citations by year
2026: 1 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2019: 3 · 2016: 6 · 2015: 3 · 2014: 3 · 2013: 1 · 2012: 3
FWCI
1.22
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
Keywords
Sema4C, semaphorins, PSD-95, postsynaptic density, PDZ domain, synaptic vesicles, neocortex, cortical neurons, axon guidance, SAP97
MeSH terms
Disks Large Homolog 4 Protein, Animals, Base Sequence, Binding Sites, Cerebral Cortex, Membrane Proteins, Nerve Tissue Proteins, Protein Binding, Subcellular Fractions, DNA Primers, Gene Expression Regulation, Developmental, Two-Hybrid System Techniques, Semaphorins, Intracellular Signaling Peptides and Proteins, Mice, Rats, Guanylate Kinases

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