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The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

In Vitro Reconstitution of an Escherichia coli RNA-guided Immune System Reveals Unidirectional, ATP-dependent Degradation of DNA Target

Mulepati, Sabin, Bailey, Scott·2013·Journal of Biological Chemistry
DOI 10.1074/jbc.m113.472233
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  • Although degradation by Cas3 handicaps the invader, it might not lead to full destruction of the target; complete degradation might be induced by other cellular nucleases or by Cascade-independent Cas3 nuclease activity.

    “Although this degradation handicaps the invader, it might not lead to the full destruction of the target. Complete degradation might be induced by other cellular nucleases or by Cascade-independent Cas3 nuclease activity, which has been previously documented [44,45,46].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [45]

    The linear-DNA experiments report that Cas3 degradation proceeds upstream from the protospacer and leaves downstream DNA intact, supporting the possibility that Cas3 does not fully destroy a target. The paper does not propose that other cellular nucleases complete degradation or document Cascade-independent Cas3 degradation of the target; its experiments describe target degradation as Cascade-dependent. The cited copy provides section labels but no page numbers. (LLM, conf=0.84)

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Research works printed but not linked: 26

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  2. Bolotin, 2005, Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin, Microbiology, 151, 2551, 10.1099/mic.0.28048-0 · DOI · Paper page
  3. Mojica, 2005, Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements, J. Mol. Evol, 60, 174, 10.1007/s00239-004-0046-3 · DOI · Paper page
  4. Makarova, 2011, Evolution and classification of the CRISPR-Cas systems, Nat. Rev. Microbiol, 9, 467, 10.1038/nrmicro2577 · DOI · Paper page
  5. Brouns, 2008, Small CRISPR RNAs guide antiviral defense in prokaryotes, Science, 321, 960, 10.1126/science.1159689 · DOI · Paper page
  6. Jore, 2011, Structural basis for CRISPR RNA-guided DNA recognition by Cascade, Nat. Struct. Mol. Biol, 18, 529, 10.1038/nsmb.2019 · DOI
  7. Wiedenheft, 2011, Structures of the RNA-guided surveillance complex from a bacterial immune system, Nature, 477, 486, 10.1038/nature10402 · DOI
  8. Semenova, 2011, Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence, Proc. Natl. Acad. Sci. U.S.A, 108, 10098, 10.1073/pnas.1104144108 · DOI · Paper page
  9. Mojica, 2009, Short motif sequences determine the targets of the prokaryotic CRISPR defence system, Microbiology, 155, 733, 10.1099/mic.0.023960-0 · DOI · Paper page
  10. Goren, 2012, Experimental definition of a clustered regularly interspaced short palindromic duplicon in Escherichia coli, J. Mol. Biol, 423, 14, 10.1016/j.jmb.2012.06.037 · DOI
  11. Sashital, 2012, Mechanism of foreign DNA selection in a bacterial adaptive immune system, Mol. Cell, 46, 606, 10.1016/j.molcel.2012.03.020 · DOI
  12. Mulepati, 2012, Crystal structure of the largest subunit of a bacterial RNA-guided immune complex and its role in DNA target binding, J. Biol. Chem, 287, 22445, 10.1074/jbc.C112.379503 · DOI
  13. Mulepati, 2011, Structural and biochemical analysis of nuclease domain of clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 3 (Cas3), J. Biol. Chem, 286, 31896, 10.1074/jbc.M111.270017 · DOI
  14. Sinkunas, 2011, Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system, EMBO J, 30, 1335, 10.1038/emboj.2011.41 · DOI
  15. Beloglazova, 2011, Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference, EMBO J, 30, 4616, 10.1038/emboj.2011.377 · DOI
  16. Howard, 2011, Helicase dissociation and annealing of RNA-DNA hybrids by Escherichia coli Cas3 protein, Biochem. J, 439, 85, 10.1042/BJ20110901 · DOI
  17. Westra, 2012, CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled invader DNA by Cascade and Cas3, Mol. Cell, 46, 595, 10.1016/j.molcel.2012.03.018 · DOI
  18. Peränen, 1996, T7 vectors with a modified T7lac promoter for expression of proteins in Escherichia coli, Anal. Biochem, 236, 371, 10.1006/abio.1996.0187 · DOI
  19. Macomber, 2011, Fructose-1,6-bisphosphate aldolase (class II) is the primary site of nickel toxicity in Escherichia coli, Mol. Microbiol, 82, 1291, 10.1111/j.1365-2958.2011.07891.x · DOI
  20. Anjem, 2009, Manganese import is a key element of the OxyR response to hydrogen peroxide in Escherichia coli, Mol. Microbiol, 72, 844, 10.1111/j.1365-2958.2009.06699.x · DOI · Paper page
  21. Kiianitsa, 2003, NADH-coupled microplate photometric assay for kinetic studies of ATP-hydrolyzing enzymes with low and high specific activities, Anal. Biochem, 321, 266, 10.1016/S0003-2697(03)00461-5 · DOI
  22. Yosef, 2011, High-temperature protein G is essential for activity of the Escherichia coli clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system, Proc. Natl. Acad. Sci. U.S.A, 108, 20136, 10.1073/pnas.1113519108 · DOI
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Metadata

Authors
Mulepati, S., & Bailey, S.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2013-08
Volume
288
Issue
31
Pages
22184-22192
ISSN
0021-9258
DOI
10.1074/jbc.m113.472233
Cited by
179
References
1
Language
en
Licence

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