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The DOI matches its Crossref record.

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

Brouns, Stan J. J., Jore, Matthijs M., Lundgren, Magnus, Westra, Edze R., Slijkhuis, Rik J. H., Snijders, Ambrosius P. L., Dickman, Mark J., Makarova, Kira S., Koonin, Eugene V., van der Oost, John·2008·Science
DOI 10.1126/science.1159689PMC PMC5898235
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Abstract

Prokaryotes acquire virus resistance by integrating short fragments of viral nucleic acid into clusters of regularly interspaced short palindromic repeats (CRISPRs). Here we show how virus-derived sequences contained in CRISPRs are used by CRISPR-associated (Cas) proteins from the host to mediate an antiviral response that counteracts infection. After transcription of the CRISPR, a complex of Cas proteins termed Cascade cleaves a CRISPR RNA precursor in each repeat and retains the cleavage products containing the virus-derived sequence. Assisted by the helicase Cas3, these mature CRISPR RNAs then serve as small guide RNAs that enable Cascade to interfere with virus proliferation. Our results demonstrate that the formation of mature guide RNAs by the CRISPR RNA endonuclease subunit of Cascade is a mechanistic requirement for antiviral defense.

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  • In type I systems a Cascade system is formed, composed of a multiprotein backbone with different Cas protein subunits linked to the crRNA molecule.

    “In type I systems a Cascade system (CRISPR-associated complex for antiviral defence) is formed, composed of a multiprotein backbone with different Cas protein subunits linked to the crRNA molecule [41].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [41]

    The passage labeled Abstract identifies Cascade in E. coli K12 as a complex of five different Cas proteins; another passage shows that crRNA copurifies with it. The copy supports the complex and its association with crRNA, but does not establish the claim for type I systems generally or describe a structural “backbone.” No page numbers are provided in our copy. (LLM, conf=0.82)

    Experimental — accuracy not yet measured

  • A group led by John van der Oost described the existence of crRNA molecules and named them crRNA.

    “A group of researchers led by John van der Oost (Wageningen University, the Netherlands), who described the existence of such RNA molecules, gave them the name crRNA (CRISPR-associated RNA).”

    From CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing (2022) · cites this paper as [30]

    In the passage describing Northern analysis (section labeled Abstract; page unavailable), the authors report detecting a small RNA product and call it “CRISPR-RNA (crRNA).” This supports the attribution that van der Oost’s group described the molecules and used the name crRNA, but not the citing sentence’s expansion of crRNA as “CRISPR-associated RNA.” (LLM, conf=0.85)

    Experimental — accuracy not yet measured

  • The initial result of transcription from the CRISPR locus is a pre-crRNA precursor molecule consisting of several spacers and repeats, which is later cleaved into individual fragments.

    “It was also noted that the initial result of transcription from the CRISPR locus is a pre-crRNA precursor molecule consisting of several spacers and repeats, which is later cleaved into individual fragments [30].”

    From CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing (2022) · cites this paper as [30]

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Citations

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Research works printed but not linked: 19

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  19. 10.1110/ps.062131106 · DOI

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Metadata

Authors
Brouns, S. J. J., Jore, M. M., Lundgren, M., Westra, E. R., Slijkhuis, R. J. H., Snijders, A. P. L., Dickman, M. J., Makarova, K. S., Koonin, E. V., & van der Oost, J.
Affiliations
Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, Netherlands.
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2008-08-15
Volume
321
Issue
5891
Pages
960-964
ISSN
  • 0036-8075
  • 1095-9203
DOI
10.1126/science.1159689
PMC
PMC5898235
Cited by
2,460
References
0
Language
en

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