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In vitro reconstitution of Cascade‐mediated CRISPR immunity in Streptococcus thermophilus

Sinkunas, Tomas, Gasiunas, Giedrius, Waghmare, Sakharam P, Dickman, Mark J, Barrangou, Rodolphe, Horvath, Philippe, Siksnys, Virginijus·2013·The EMBO Journal
DOI 10.1038/emboj.2012.352
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  • Although degradation by Cas3 handicaps the invader, it might not lead to full destruction of the target; complete degradation might be induced by other cellular nucleases or by Cascade-independent Cas3 nuclease activity.

    “Although this degradation handicaps the invader, it might not lead to the full destruction of the target. Complete degradation might be induced by other cellular nucleases or by Cascade-independent Cas3 nuclease activity, which has been previously documented [44,45,46].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [46]

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Research works printed but not linked: 35

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  10. Gasiunas G, Barrangou R, Horvath P, Siksnys V (2012) Cas9‐crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc Natl Acad Sci USA 109: E2579–E2586 · DOI · Paper page
  11. Goren MG, Yosef I, Auster O, Qimron U (2012) Experimental Definition of a Clustered Regularly Interspaced Short Palindromic Duplicon in Escherichia coli. J Mol Biol 423: 14–16 · DOI
  12. Hale CR, Majumdar S, Elmore J, Pfister N, Compton M, Olson S, Resch AM, Glover CV 3rd, Graveley BR, Terns RM, Terns MP (2012) Essential features and rational design of CRISPR RNAs that function with the Cas RAMP module complex to cleave RNAs. Mol Cell 45: 292–302 · DOI
  13. Horvath P, Barrangou R (2010) CRISPR/Cas, the immune system of bacteria and archaea. Science 327: 167–170 · DOI
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  15. Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A (1987) Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol 169: 5429–5433 · DOI · Paper page
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  18. Labrie SJ, Samson JE, Moineau S (2010) Bacteriophage resistance mechanisms. Nat Rev Microbiol 8: 317–327 · DOI
  19. Makarova KS, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineau S, Mojica FJM, Wolf YI, Yakunin AF, van der Oost J, Koonin EV (2011) Evolution and classification of the CRISPR‐Cas systems. Nat Rev Microbiol 9: 467–477 · DOI · Paper page
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Metadata

Authors
Sinkunas, T., Gasiunas, G., Waghmare, S. P., Dickman, M. J., Barrangou, R., Horvath, P., & Siksnys, V.
Venue
The EMBO Journal
Publisher
Springer Science and Business Media LLC
Published
2013-01-18
Volume
32
Issue
3
Pages
385-394
ISSN
  • 0261-4189
  • 1460-2075
DOI
10.1038/emboj.2012.352
Cited by
228
References
3
Language
en
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