Structure and Biosynthesis of Two Exopolysaccharides Produced by Lactobacillus johnsonii FI9785
Open accessAbstract
Exopolysaccharides were isolated and purified from Lactobacillus johnsonii FI9785, which has previously been shown to act as a competitive exclusion agent to control Clostridium perfringens in poultry. Structural analysis by NMR spectroscopy revealed that L. johnsonii FI9785 can produce two types of exopolysaccharide: EPS-1 is a branched dextran with the unusual feature that every backbone residue is substituted with a 2-linked glucose unit, and EPS-2 was shown to have a repeating unit with the following structure: -6)-α-Glc p -(1–3)-β-Glc p -(1–5)-β-Gal f -(1–6)-α-Glc p -(1–4)-β-Gal p -(1–4)-β-Glc p -(1-. Sites on both polysaccharides were partially occupied by substituent groups: 1-phosphoglycerol and O -acetyl groups in EPS-1 and a single O -acetyl group in EPS-2. Analysis of a deletion mutant (Δ epsE ) lacking the putative priming glycosyltransferase gene located within a predicted eps gene cluster revealed that the mutant could produce EPS-1 but not EPS-2, indicating that epsE is essential for the biosynthesis of EPS-2. Atomic force microscopy confirmed the localization of galactose residues on the exterior of wild type cells and their absence in the Δ epsE mutant. EPS2 was found to adopt a random coil structural conformation. Deletion of the entire 14-kb eps cluster resulted in an acapsular mutant phenotype that was not able to produce either EPS-2 or EPS-1. Alterations in the cell surface properties of the EPS-specific mutants were demonstrated by differences in binding of an anti-wild type L. johnsonii antibody. These findings provide insights into the biosynthesis and structures of novel exopolysaccharides produced by L. johnsonii FI9785, which are likely to play an important role in biofilm formation, protection against harsh environment of the gut, and colonization of the host. Background: Bacterial cell surface polysaccharides are important in pathogenesis, cell adhesion, and protection against harsh environments. Results: Two novel exopolysaccharide (EPS) structures were identified in Lactobacillus johnsonii . Conclusion: The eps cluster is essential for production of both EPS, but epsE is required only for the heteropolymer. Significance: This study will guide functional analysis of EPS in survival and colonization of gut commensals.
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EPS produced by Lb. johnsonii FI9785 may favor colonization of probiotic bacteria in the host gastrointestinal tract by replacing pathogenic bacteria in a competitive inhibition mode.
“EPS produced by Lb. johnsonii FI9785 may favor the colonization of probiotic bacteria in the host gastrointestinal tract by replacing pathogenic bacteria in a competitive inhibition mode [145].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [145]
The Introduction says L. johnsonii FI9785 out-competes pathogens in challenge models, while the Discussion suggests its EPS may aid survival during gut transit and affect colonization. The paper says the mechanism of pathogen exclusion is unknown and leaves the EPS’s role in it for future study; it does not establish that EPS replaces pathogens through competitive inhibition. (LLM, conf=0.88)
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Cites
Statistical mechanics of chain molecules
Flory, P. J., & Volkenstein, M. V. (1969) · Biopolymers · doi:10.1002/bip.1969.360080514 · Cited by 6,449 · see citing works
Calibration of atomic-force microscope tips
Hutter, J. L., & Bechhoefer, J. (1993) · Review of Scientific Instruments · doi:10.1063/1.1143970 · Cited by 4,205 · see citing works
Röntgenuntersuchung gelöster Fadenmoleküle
Kratky, O., & Porod, G. (1949) · Recueil des Travaux Chimiques des Pays-Bas · doi:10.1002/recl.19490681203 · Cited by 1,988 · see citing works
Heteropolysaccharides from lactic acid bacteria
Vuyst, L. D., & Degeest, B. (1999) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.1999.tb00395.x · Cited by 840 · see citing works
Genetic Analysis of the Capsular Biosynthetic Locus from All 90 Pneumococcal Serotypes
Bentley, S. D., Aanensen, D. M., Mavroidi, A., Saunders, D., Rabbinowitsch, E., Collins, M. D., Donohoe, K., Harris, D. J., Murphy, L., Quail, M. A., et al. (2006) · PLoS Genetics · doi:10.1371/journal.pgen.0020031 · Cited by 759 · see citing works
Fanning, S., Hall, L. J., Cronin, M. A., Zomer, A., MacSharry, J. A., Goulding, D., Motherway, M. O., Shanahan, F., Nally, K., Dougan, G., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1115621109 · Cited by 564 · see citing works
New Perspectives for Lactobacilli Exopolysaccharides
Badel, S., Bernardi, T., & Michaud, P. (2011) · Biotechnology Advances · doi:10.1016/j.biotechadv.2010.08.011 · Cited by 497 · see citing works
Efficient insertional mutagenesis in lactococci and other gram-positive bacteria
Maguin, E., Prévost, H., Ehrlich, S. D., & Gruss, A. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.3.931-935.1996 · Cited by 486 · see citing works
van Hijum, S. A. F. T., Kralj, S., Ozimek, L. K., Dijkhuizen, L., & van Geel-Schutten, I. H. (2006) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.70.1.157-176.2006 · Cited by 466 · see citing works
Statistical mechanics of chain molecules
Volkenstein, M. (1970) · Biopolymers · doi:10.1002/bip.1970.360090311 · Cited by 446 · see citing works
Nanoscale mapping and functional analysis of individual adhesins on living bacteria
Duprès, V., Menozzi, F. D., Locht, C., Clare, B. H., Abbott, N. L., Cuenot, S., Bompard, C., Raze, D., & Dufrêne, Y. F. (2005) · Nature Methods · doi:10.1038/nmeth769 · Cited by 334 · see citing works
The biosynthesis and functionality of the cell-wall of lactic acid bacteria.
Delcour, J., Ferain, T., Deghorain, M., Palumbo, E., & Pascal Hols, A. (1999) · PubMed · Cited by 286 · see citing works
Lebeer, S., Verhoeven, T. L. A., Francius, G., Schoofs, G., Lambrichts, I., Dufrêne, Y. F., Vanderleyden, J., & Keersmaecker, S. C. J. D. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.02919-08 · Cited by 271 · see citing works
van Kranenburg, R., Marugg, J. D., van Swam, I. I., Willem, N. J., & de Vos, W. M. (1997) · Molecular Microbiology · doi:10.1046/j.1365-2958.1997.3521720.x · Cited by 262 · see citing works
The biosynthesis and functionality of the cell-wall of lactic acid bacteria
Delcour, J., Ferain, T., Deghorain, M., Palumbo, E., & Pascal Hols, A. (1999) · Antonie van Leeuwenhoek · doi:10.1023/a:1002089722581 · Cited by 262 · see citing works
Isolation and Characterization of Two Exopolysaccharides Produced by Lactobacillus plantarum EP56
Tallon, R., Bressollier, P., & Urdaci, M. C. (2003) · Research in Microbiology · doi:10.1016/j.resmic.2003.09.006 · Cited by 260 · see citing works
Lebeer, S., Verhoeven, T. L. A., Vélez, M. P., Vanderleyden, J., & Keersmaecker, S. C. J. D. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.01393-07 · Cited by 258 · see citing works
Ragione, R. M. L., Narbad, A., Gasson, M. J., & Woodward, M. J. (2004) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2004.01474.x · Cited by 252 · see citing works
Kralj, S., van Geel-Schutten, G. H., Dondorff, M. M. G., Kirsanovs, S., van der Maarel, M. J. E. C., & Dijkhuizen, L. (2004) · Microbiology · doi:10.1099/mic.0.27321-0 · Cited by 213 · see citing works
Calibration of atomic force microscope
Masalska, A., Zawierucha, P., Woszczyna, M., Gotszalk, T. P., Ritz, Y., & Zschech, E. (2008) · Elektronika : konstrukcje, technologie, zastosowania · Cited by 211 · see citing works
Kitazawa, H., Harata, T., Uemura, J., Saito, T., Kaneko, T., & Itoh, T. (1998) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(98)00030-0 · Cited by 205 · see citing works
MacKenzie, D. A., Jeffers, F., Parker, M. L., Vibert-Vallet, A., Bongaerts, R. J., Roos, S., Walter, J., & Juge, N. (2010) · Microbiology · doi:10.1099/mic.0.043265-0 · Cited by 202 · see citing works
Maina, N. H., Tenkanen, M., Maaheimo, H., Juvonen, R., & Virkki, L. (2008) · Carbohydrate Research · doi:10.1016/j.carres.2008.04.012 · Cited by 200 · see citing works
Physiological function of exopolysaccharides produced by Lactococcus lactis
Looijesteijn, P. J., Trapet, L., de Vries, E., Abee, T., & Hugenholtz, J. (2001) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(00)00437-2 · Cited by 194 · see citing works
Hidalgo-Cantabrana, C., López, P., Gueimonde, M., Reyes‐Gavilán, C. G. D. L., Suárez, A., Margollés, A., & Ruas‐Madiedo, P. (2012) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-012-9110-2 · Cited by 192 · see citing works
Denou, E., Pridmore, R. D., Berger, B. W., Panoff, J.-M., Arigoni, F., & Brüssow, H. (2008) · Journal of Bacteriology · doi:10.1128/jb.01637-07 · Cited by 179 · see citing works
Cieslewicz, M. J., Kasper, D. L., Wang, Y., & Wessels, M. R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m005702200 · Cited by 162 · see citing works
The revised NMR chemical shift data of carrageenans
van de Velde, F., Pereira, L., & Rollema, H. S. (2004) · Carbohydrate Research · doi:10.1016/j.carres.2004.07.015 · Cited by 157 · see citing works
Kralj, S., van Geel-Schutten, G. H., Rahaoui, H., Leer, R. J., Faber, E. J., van der Maarel, M. J. E. C., & Dijkhuizen, L. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.9.4283-4291.2002 · Cited by 126 · see citing works
Structure and functions of exopolysaccharide produced by gut commensal Lactobacillus reuteri 100-23
Sims, I. M., Frese, S. A., Walter, J., Loach, D. M., Wilson, M., Appleyard, K., Eason, J. R., Livingston, M. K., Baird, M. A., Cook, G. M., et al. (2011) · The ISME Journal · doi:10.1038/ismej.2010.201 · Cited by 123 · see citing works
van Leeuwen, S. S., Kralj, S., van Geel-Schutten, I. H., Gerwig, G. J., Dijkhuizen, L., & Kamerling, J. P. (2008) · Carbohydrate Research · doi:10.1016/j.carres.2008.01.042 · Cited by 121 · see citing works
Minić, Z., Marie, C., Delorme, C., Faurie, J., Mercier, G., Ehrlich, S. D., & Renault, P. (2007) · Journal of Bacteriology · doi:10.1128/jb.01122-06 · Cited by 95 · see citing works
Morona, J. K., Morona, R., Miller, D. C., & Paton, J. C. (2003) · Journal of Bacteriology · doi:10.1128/jb.185.10.3009-3019.2003 · Cited by 89 · see citing works
Determination of the structure of the exopolysaccharide produced by Lactobacillus sake 0–1
Robijn, G. W., van den Berg, D. J. C., Haas, H., Kamerling, J. P., & Vliegenthart, J. F. G. (1995) · Carbohydrate Research · doi:10.1016/0008-6215(95)00172-p · Cited by 86 · see citing works
Lin, M., Yang, Y., Chen, Y., Hua, K., Lu, C., Sheu, F., Lin, G.-H., Tsay, S., Liang, S., & Wu, S. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.200113 · Cited by 78 · see citing works
Structure of the exopolysaccharide produced by Streptococcus thermophilus S3
Faber, E. J., van den Haak, M. J., Kamerling, J. P., & Vliegenthart, J. F. G. (2001) · Carbohydrate Research · doi:10.1016/s0008-6215(01)00013-1 · Cited by 74 · see citing works
Horn, N., Wegmann, U., Dertli̇, E., Mulholland, F., Collins, S. R. A., Waldron, K. W., Bongaerts, R. J., Mayer, M. J., & Narbad, A. (2013) · PLoS ONE · doi:10.1371/journal.pone.0059957 · Cited by 72 · see citing works
Systemic antibody responses to gut commensal bacteria during chronic HIV-1 infection
Haas, A., Zimmermann, K., Graw, F., Slack, E. M. C., Rusert, P., Ledergerber, B., Bossart, W., Weber, R. K., Thurnheer, M. C., Battegay, M., et al. (2011) · Gut · doi:10.1136/gut.2010.224774 · Cited by 71 · see citing works
Structural studies of the exopolysaccharide produced by Lactobacillus paracasei 34-1
Robijn, G. W., Wienk, H., van den Berg, D. J. C., Haas, H., Kamerling, J. P., & Vliegenthart, J. F. G. (1996) · Carbohydrate Research · doi:10.1016/s0008-6215(96)90178-0 · Cited by 66 · see citing works
Wegmann, U., Klein, J., Drumm, I., Kuipers, O. P., & Henrich, B. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.11.4729-4733.1999 · Cited by 65 · see citing works
Górska, S., Jachymek, W., Rybka, J., Strus, M., Heczko, P. B., & Gamian, A. (2009) · Carbohydrate Research · doi:10.1016/j.carres.2009.09.015 · Cited by 64 · see citing works
Bacteria Survive Multiple Puncturings of Their Cell Walls
Suo, Z., Avci, R., Deliorman, M., Yang, X., & Pascual, D. W. (2009) · Langmuir · doi:10.1021/la8033319 · Cited by 61 · see citing works
Sato, T., Uemura, J., Shimosato, T., Kawai, Y., Kitazawa, H., & Saito, T. (2004) · Journal of Food Protection · doi:10.4315/0362-028x-67.8.1719 · Cited by 57 · see citing works
Structural elucidation of the viscous exopolysaccharide produced by Lactobacillus helveticus Lb161
Staaf, M., Yang, Z., Huttunen, E., & Widmalm, G. (2000) · Carbohydrate Research · doi:10.1016/s0008-6215(00)00027-6 · Cited by 53 · see citing works
Paulo, E. M., Boffo, E. F., Branco, A., Valente, A. M. M. P., de Melo, I. S., Ferreira, A. G., Roque, M. R. A., & de Assis, S. A. (2012) · Anais da Academia Brasileira de Ciências · doi:10.1590/s0001-37652012000200018 · Cited by 50 · see citing works
Lactobacillus helveticus glycosyltransferases: from genes to carbohydrate synthesis
Jolly, L., Newell, J., Porcelli, I., Vincent, S. J. F., & Stingele, F. (2002) · Glycobiology · doi:10.1093/glycob/12.5.319 · Cited by 48 · see citing works
Structure of the high-molecular weight exopolysaccharide isolated from Lactobacillus pentosus LPS26
Rodríguez-Carvajal, M. Á., Sánchez, J.-I., Campelo, A. B., Martı́nez, B., Rodrı́guez, A., & Serrano, A. M. G. (2008) · Carbohydrate Research · doi:10.1016/j.carres.2008.08.028 · Cited by 46 · see citing works
Features and applications of bacterial glycosyltransferases: current state and prospects
Luzhetskyy, A. N., & Bechthold, A. (2008) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-008-1672-2 · Cited by 45 · see citing works
HEPES-Stabilized Encapsulation of Salmonella typhimurium
Suo, Z., Yang, X., Avci, R., Kellerman, L., Pascual, D. W., Fries, M. D., & Steele, A. (2006) · Langmuir · doi:10.1021/la0621721 · Cited by 39 · see citing works
Deutsch, S., Parayre, S., Bouchoux, A., Guyomarc’H, F., Dewulf, J., Dols‐Lafargue, M., Baglinière, F., Cousin, F., Falentin, H., Jan, G., et al. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.07027-11 · Cited by 38 · see citing works
Sutherland, I. W., & Wilkinson, J. F. (1968) · Biochemical Journal · doi:10.1042/bj1100749 · Cited by 36 · see citing works
Gunning, A. P., Chambers, S. J., Pin, C., Man, A., Morris, V. J., & Nicoletti, C. (2008) · The FASEB Journal · doi:10.1096/fj.07-100578 · Cited by 35 · see citing works
Avcı, G. A., Aslım, B., Suludere, Z., & Akca, G. (2010) · Microbiology and Immunology · doi:10.1111/j.1348-0421.2010.00227.x · Cited by 28 · see citing works
Structural investigation of the exopolysaccharide produced by Pseudomonas flavescens strain B62
Cescutti, P., Toffanin, R., Fett, W. F., Osman, S. F., Pollesello, P., & Paoletti, S. (1998) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.1998.2510971.x · Cited by 22 · see citing works
Characterisation of a novel plasmid p9785S from Lactobacillus johnsonii FI9785
Horn, N., Wegmann, U., Narbad, A., & Gasson, M. J. (2005) · Plasmid · doi:10.1016/j.plasmid.2005.01.005 · Cited by 16 · see citing works
High-resolution J-coupled 13C MAS NMR spectroscopy of lipid membranes
Ciesielski, F., Griffin, D. C., Rittig, M. G., & Bonev, B. B. (2009) · Chemistry and Physics of Lipids · doi:10.1016/j.chemphyslip.2009.07.001 · Cited by 14 · see citing works
Sutherland, I. W., Jann, K., & Jann, B. (1970) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1970.tb00848.x · Cited by 14 · see citing works
Iwamori, M., Shibagaki, T., Nakata, Y., Adachi, S., & Nomura, T. (2009) · The Journal of Biochemistry · doi:10.1093/jb/mvp058 · Cited by 12 · see citing works
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Polysaccharide Production by Lactic Acid Bacteria: From Genes to Industrial Applications
Zeidan, A. A., Poulsen, V. K., Janzen, T., Buldo, P., Derkx, P. M. F., Øregaard, G., & Neves, A. R. (2017) · FEMS Microbiology Reviews · doi:10.1093/femsre/fux017 · Cited by 326 · see citing works
Castro-Bravo, N., Wells, J. M., Margolles, A., & Ruas-Madiedo, P. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02426 · Cited by 284 · see citing works
The Critical Roles of Polysaccharides in Gut Microbial Ecology and Physiology
Porter, N. T., & Martens, E. C. (2017) · Annual Review of Microbiology · doi:10.1146/annurev-micro-102215-095316 · Cited by 251 · see citing works
Nanoencapsulation for Probiotic Delivery
Centurion, F., Basit, A. W., Liu, J., Gaisford, S., Rahim, M. A., & Kalantar‐Zadeh, K. (2021) · ACS Nano · doi:10.1021/acsnano.1c09951 · Cited by 225 · see citing works
Dertli̇, E., Mayer, M. J., & Narbad, A. (2015) · BMC Microbiology · doi:10.1186/s12866-015-0347-2 · Cited by 216 · see citing works
Liu, Z., Zhang, Z., Qiu, L., Zhang, F., Xu, X., Wei, H., & Tao, X. (2017) · Journal of Dairy Science · doi:10.3168/jds.2016-11944 · Cited by 205 · see citing works
Vandana, & Das, S. (2022) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2022.119536 · Cited by 199 · see citing works
Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods
Prete, R., Alam, M. K., Perpetuini, G., Perla, C., Pittia, P., & Corsetti, A. (2021) · Foods · doi:10.3390/foods10071653 · Cited by 194 · see citing works
Bacteria Facilitate Enteric Virus Co-infection of Mammalian Cells and Promote Genetic Recombination
Erickson, A. K., Jesudhasan, P. R. R., Mayer, M. J., Narbad, A., Winter, S., & Pfeiffer, J. K. (2017) · Cell Host & Microbe · doi:10.1016/j.chom.2017.11.007 · Cited by 187 · see citing works
Xu, Y., Cui, Y., Yue, F., Liu, L., Shan, Y., Liu, B., Zhou, Y., & Lü, X. (2019) · Food Hydrocolloids · doi:10.1016/j.foodhyd.2019.03.032 · Cited by 189 · see citing works
Dertli, E., Mercan, E., Arıcı, M., Yılmaz, M. T., & Sağdıç, O. (2016) · LWT - Food Science and Technology · doi:10.1016/j.lwt.2016.03.030 · Cited by 168 · see citing works
Benefits of bacteria-derived exopolysaccharides on gastrointestinal microbiota, immunity and health
Oerlemans, M. M. P., Akkerman, R., Ferrari, M. D. L., Walvoort, M. T. C., & de Vos, P. (2020) · Journal of Functional Foods · doi:10.1016/j.jff.2020.104289 · Cited by 154 · see citing works
da Silva, L. A., Neto, J. H. P. L., & Cardarelli, H. R. (2019) · Annals of Microbiology · doi:10.1007/s13213-019-01456-9 · Cited by 152 · see citing works
Taylan, O., Yılmaz, M. T., & Dertli̇, E. (2019) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.06.078 · Cited by 126 · see citing works
Werning, M. L., Hernández-Alcántara, A. M., Ruiz, M. J., Soto, L. P., Dueñas, M. T., López, P., & Frizzo, L. S. (2022) · Foods · doi:10.3390/foods11091284 · Cited by 126 · see citing works
Protein-Polysaccharide Interactions to Alter Texture
van de Velde, F., de Hoog, E. H. A., Oosterveld, A., & Tromp, R. H. (2015) · Annual Review of Food Science and Technology · doi:10.1146/annurev-food-022814-015558 · Cited by 116 · see citing works
Hatziioanou, D., Gherghisan-Filip, C., Saalbach, G., Horn, N., Wegmann, U., Duncan, S. H., Flint, H. J., Mayer, M. J., & Narbad, A. (2017) · Microbiology · doi:10.1099/mic.0.000515 · Cited by 113 · see citing works
Bibi, A., Xiong, Y., Rajoka, M. S. R., Mehwish, H. M., Radicetti, E., Umair, M., Shoukat, M., Khan, M. K. I., & Aadil, R. M. (2021) · Sustainability · doi:10.3390/su132212429 · Cited by 103 · see citing works
Lactobacillus-produced exopolysaccharides and their potential health benefits: A review
Patten, D. A., & Laws, A. P. (2015) · Beneficial Microbes · doi:10.3920/bm2014.0117 · Cited by 100 · see citing works
Dertli̇, E., Toker, Ö. S., Durak, M. Z., Yılmaz, M. T., Tatlısu, N. B., Sağdıç, O., & Cankurt, H. (2015) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2015.08.047 · Cited by 98 · see citing works
Yılmaz, M. T., Dertli̇, E., Toker, Ö. S., Tatlısu, N. B., Sağdıç, O., & Arıcı, M. (2014) · Journal of Dairy Science · doi:10.3168/jds.2014-8936 · Cited by 92 · see citing works
Deo, D., Davray, D., & Kulkarni, R. (2019) · Microorganisms · doi:10.3390/microorganisms7100444 · Cited by 90 · see citing works
Next-Generation Probiotics and Chronic Diseases: A Review of Current Research and Future Directions
Tiwari, A. K., Krisnawati, D. I., Susilowati, E., Mutalik, C., & Kuo, T. (2024) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.4c08702 · Cited by 88 · see citing works
Structural analysis of the α-d-glucan produced by the sourdough isolate Lactobacillus brevis E25
Dertli̇, E., Colquhoun, I. J., Côté, G. L., Gall, G. L., & Narbad, A. (2017) · Food Chemistry · doi:10.1016/j.foodchem.2017.09.017 · Cited by 80 · see citing works
İspirli, H., Sagdic, O., Yılmaz, M. T., & Dertli, E. (2019) · Process Biochemistry · doi:10.1016/j.procbio.2018.12.015 · Cited by 77 · see citing works
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Dimopoulou, M., Vuillemin, M., Campbell-Sills, H., Lucas, P., Ballestra, P., Miot‐Sertier, C., Favier, M., Coulon, J., Moine, V., Doco, T., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0098898 · Cited by 75 · see citing works
Tiwari, O. N., Sasmal, S., Kataria, A. K., & Devi, I. (2020) · 3 Biotech · doi:10.1007/s13205-020-02200-w · Cited by 75 · see citing works
İspirli, H., Özmen, D., Yılmaz, M. T., Sağdıç, O., & Dertli̇, E. (2019) · Food Control · doi:10.1016/j.foodcont.2019.106812 · Cited by 67 · see citing works
Deka, P. C., Goswami, G., Das, P., Gautom, T., Chowdhury, N., Boro, R. C., & Barooah, M. (2018) · Molecular Biology Reports · doi:10.1007/s11033-018-4566-0 · Cited by 66 · see citing works
Sengupta, D., Datta, S., & Biswas, D. (2018) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-018-8745-7 · Cited by 60 · see citing works
Aburas, H. M., İspirli, H., Taylan, O., Yılmaz, M. T., & Dertli̇, E. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.06.030 · Cited by 59 · see citing works
Potential Health Benefits of Ropy Exopolysaccharides Produced by Lactobacillus plantarum
Yılmaz, T., & Şimşek, Ö. (2020) · Molecules · doi:10.3390/molecules25143293 · Cited by 54 · see citing works
Dertli̇, E., Yılmaz, M. T., Tatlısu, N. B., Toker, Ö. S., Cankurt, H., & Sağdıç, O. (2016) · Meat Science · doi:10.1016/j.meatsci.2016.06.008 · Cited by 54 · see citing works
Sran, K. S., Bisht, B., Mayilraj, S., & Choudhury, A. R. (2019) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.03.016 · Cited by 53 · see citing works
Debnath, S., Chakraborty, S., Langthasa, M., Choure, K., Agnihotri, V., Srivastava, A., Kant, P., Tilwari, A., Maheshwari, D. K., & Pandey, P. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1152875 · Cited by 51 · see citing works
Richards, P. J., Lafontaine, G. M. F., Connerton, P. L., Liang, L., Asiani, K., Fish, N. M., & Connerton, I. F. (2020) · mSystems · doi:10.1128/msystems.00827-19 · Cited by 48 · see citing works
Özkan, E. R., Demirci, T., & Akın, N. (2021) · LWT · doi:10.1016/j.lwt.2021.110908 · Cited by 50 · see citing works
Immunomodulatory Roles of Polysaccharide Capsules in the Intestine
Hsieh, S. A., & Allen, P. M. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.00690 · Cited by 49 · see citing works
Liao, N., Pang, B., Han, J., Xu, X., Yan, L., Li, H., Shao, D., & Shi, J. (2020) · Journal of Controlled Release · doi:10.1016/j.jconrel.2020.04.022 · Cited by 48 · see citing works
Castro‐Alves, V., Gomes, D., Menolli, N., Sforça, M. L., & do Nascimento, J. R. O. (2016) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2016.11.059 · Cited by 47 · see citing works
Banerjee, A., Das, D., Rudra, S. G., Mazumder, K., Andler, R., & Bandopadhyay, R. (2019) · Journal of Polymers and the Environment · doi:10.1007/s10924-019-01602-z · Cited by 47 · see citing works
Johnson, C., Ridley, H., Marchetti, R., Silipo, A., Griffin, D. C., Crawford, L., Bonev, B. B., Molinaro, A., & Lakey, J. H. (2014) · Molecular Microbiology · doi:10.1111/mmi.12568 · Cited by 45 · see citing works
Zhai, Q., Tian, F., Wang, G., Zhao, J., Liu, X., Cross, K. L., Zhang, H., Narbad, A., & Chen, W. (2016) · RSC Advances · doi:10.1039/c5ra24843d · Cited by 43 · see citing works
Özkan, E. R., Demirci, T., Öztürk, H. İ., & Akın, N. (2020) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.10909 · Cited by 43 · see citing works
Yalmancı, D., İspirli, H., & Dertli̇, E. (2022) · Food Bioscience · doi:10.1016/j.fbio.2022.102014 · Cited by 42 · see citing works
Laffargue, T., Moulis, C., & Remaud‐Siméon, M. (2023) · Biotechnology Advances · doi:10.1016/j.biotechadv.2023.108140 · Cited by 41 · see citing works
Structural and bioactive characteristics of a dextran produced by Lactobacillus kunkeei AK1
Yılmaz, M. T., İspirli, H., Taylan, O., Bilgrami, A. L., & Dertli̇, E. (2022) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2022.01.012 · Cited by 41 · see citing works
İspirli, H., Yüzer, M. O., Skory, C. D., Colquhoun, I. J., Sağdıç, O., & Dertli̇, E. (2019) · Biocatalysis and Biotransformation · doi:10.1080/10242422.2019.1593969 · Cited by 40 · see citing works
Bioactive and technological properties of an α-D-glucan synthesized by Weissella cibaria PDER21
Yılmaz, M. T., İspirli, H., Taylan, O., Alamoudi, M., & Dertli̇, E. (2022) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2022.119227 · Cited by 40 · see citing works
Xiao, L., Xu, D., Tang, N., Rui, X., Zhang, Q., Chen, X., Dong, M., & Li, W. (2021) · Food Chemistry Molecular Sciences · doi:10.1016/j.fochms.2021.100054 · Cited by 40 · see citing works
Yılmaz, M. T., İspirli, H., Taylan, O., Taşdemir, V., Sağdıç, O., & Dertli̇, E. (2021) · Food Bioscience · doi:10.1016/j.fbio.2021.101330 · Cited by 39 · see citing works
EpsA is an essential gene in exopolysaccharide production in L actobacillus johnsonii FI9785
Dertli̇, E., Mayer, M. J., Colquhoun, I. J., & Narbad, A. (2015) · Microbial Biotechnology · doi:10.1111/1751-7915.12314 · Cited by 39 · see citing works
Živković, M., Hidalgo-Cantabrana, C., Kojić, M., Gueimonde, M., Golić, N., & Ruas‐Madiedo, P. (2015) · Food Research International · doi:10.1016/j.foodres.2015.05.012 · Cited by 38 · see citing works
Singh, S., Sran, K. S., Pinnaka, A. K., & Choudhury, A. R. (2019) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2019.06.080 · Cited by 36 · see citing works
Şentürk, D. Z., Dertli̇, E., Erten, H., & Şimşek, Ö. (2019) · Food Science and Biotechnology · doi:10.1007/s10068-019-00641-5 · Cited by 36 · see citing works
Yang, Y., Jiang, G., & Tian, Y. (2023) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-023-03610-7 · Cited by 39 · see citing works
İspirli, H., Demirbaş, F., & Dertli̇, E. (2018) · Journal of Food Science and Technology · doi:10.1007/s13197-018-3181-3 · Cited by 35 · see citing works
Isolation and identification of exopolysaccharide producer lactic acid bacteria from Turkish yogurt
İspirli, H., & Dertli̇, E. (2017) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.13351 · Cited by 34 · see citing works
Putative Adhesion Factors in Vaginal Lactobacillus gasseri DSM 14869: Functional Characterization
Zeng, Z., Zuo, F., & Marcotte, H. (2019) · Applied and Environmental Microbiology · doi:10.1128/aem.00800-19 · Cited by 34 · see citing works
Xiao, L., Yang, Y., Han, S., Rui, X., Ma, K., Zhang, C., Wang, G., & Li, W. (2022) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2022.10.124 · Cited by 32 · see citing works
Nakamura, S., Kurata, R., Tonozuka, T., Funane, K., Park, E. Y., & Miyazaki, T. (2023) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2023.104885 · Cited by 31 · see citing works
Zhou, J., Yan, B., Wu, Y., Zhu, H., Lian, H., Zhao, J., Zhang, H., Chen, W., & Fan, D. (2021) · LWT · doi:10.1016/j.lwt.2021.111396 · Cited by 30 · see citing works
Özpınar, F. B., İspirli, H., Kayacan, S., Korkmaz, K., Dere, S., Sağdıç, O., Alkay, Z., Tunçil, Y. E., Ayyash, M. M., & Dertli̇, E. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.130507 · Cited by 29 · see citing works
Mayer, M. J., D’Amato, A., Colquhoun, I. J., Gall, G. L., & Narbad, A. (2020) · Applied and Environmental Microbiology · doi:10.1128/aem.02808-19 · Cited by 28 · see citing works
Food Supplements to Mitigate Detrimental Effects of Pelvic Radiotherapy
Segers, C., Verslegers, M., Baatout, S., Leys, N., Lebeer, S., & Mastroleo, F. (2019) · Microorganisms · doi:10.3390/microorganisms7040097 · Cited by 27 · see citing works
Revealing the Compact Structure of Lactic Acid Bacterial Heteroexopolysaccharides by SAXS and DLS
Khan, S., Birch, J., Harris, P., Calsteren, M. V., Ipsen, R., Peters, G. H. J., Svensson, B., & Almdal, K. (2017) · Biomacromolecules · doi:10.1021/acs.biomac.6b01597 · Cited by 26 · see citing works
Lactic acid bacteria - derived exopolysaccharide: Biosynthesis and antibacterial characterization
Zhang, M., Zhao, D., Yang, H., Jiao, X., Zhou, R., Zheng, J., & Wu, C. (2025) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2025.105033 · Cited by 26 · see citing works
İspirli, H., Demirbaş, F., Yüzer, M. O., & Dertli̇, E. (2018) · Food Biotechnology · doi:10.1080/08905436.2018.1507913 · Cited by 24 · see citing works
He, Y., Chen, H., Ye, Z., Zhang, X., Ye, H., & Ye, M. (2022) · Process Biochemistry · doi:10.1016/j.procbio.2022.08.035 · Cited by 24 · see citing works
Debnath, S., Das, A., Maheshwari, D. K., & Pandey, P. (2022) · Microbiological Research · doi:10.1016/j.micres.2022.127255 · Cited by 24 · see citing works
Updates on Metabolism in Lactic Acid Bacteria in Light of “Omic” Technologies
Kowalczyk, M., Mayo, B., Fernández, M., & Aleksandrzak‐Piekarczyk, T. (2015) · Wiley eBooks · doi:10.1002/9781118868386.ch1 · Cited by 25 · see citing works
Isolation and characterisation of lactic acid bacteria from traditional koumiss and kurut
İspirli, H., & Dertli̇, E. (2017) · International Journal of Food Properties · doi:10.1080/10942912.2017.1372473 · Cited by 22 · see citing works
Le, T.-S., Nguyen, P.-T., Nguyen-Ho, S.-H., Nguyen, T.-P., Nguyen, T.-T., Thai, M.-N., Nguyen-Thi, T.-U., Nguyen, M.-C., Hoang, Q.-K., & Nguyen, H. (2021) · Archives of Microbiology · doi:10.1007/s00203-021-02479-0 · Cited by 21 · see citing works
Mercan, E., İspirli, H., Sert, D., Yılmaz, M. T., & Dertli̇, E. (2015) · Archives of Microbiology · doi:10.1007/s00203-015-1141-0 · Cited by 21 · see citing works
Yalmancı, D., Dertli̇, E., Tekin‐Cakmak, Z. H., & Karasu, S. (2023) · International Journal of Food Science & Technology · doi:10.1111/ijfs.16287 · Cited by 18 · see citing works
Ermiş, E., Poyraz, E., Dertli̇, E., & Yılmaz, M. T. (2020) · Sakarya University Journal of Science · doi:10.16984/saufenbilder.545929 · Cited by 18 · see citing works
Yalmancı, D., Dertli̇, E., Tekin‐Cakmak, Z. H., & Karasu, S. (2022) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2022.12.069 · Cited by 17 · see citing works
Do, T. B. T., Tran, B.-K., Trần, T. V. T., Hiếu, L. T., Cnockaert, M. C., Vandamme, P. A. R., Nguyen, T. H. C., Nguyen, C. C., Hong, S. H., Kim, S. Y., et al. (2020) · ACS Omega · doi:10.1021/acsomega.0c05256 · Cited by 16 · see citing works
Knirel, Y. A., & Calsteren, M. V. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-819475-1.00005-5 · Cited by 16 · see citing works
İspirli, H., Sağdıç, O., & Dertli̇, E. (2020) · Preparative Biochemistry & Biotechnology · doi:10.1080/10826068.2020.1795673 · Cited by 15 · see citing works
A contextualised protein language model reveals the functional syntax of bacterial evolutionPreprint
Wiatrak, M., Abelson, D. C., Mikhaylenko, R., Torné, R. V., Ntemourtsidou, M., Dinan, A. M., Arora, D., Horsfield, S., Lees, J. A., Brbić, M., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.07.20.665723 · Cited by 16 · see citing works
Sample Collection and Preparation of Biofluids and Extracts for NMR Spectroscopy
Gall, G. L. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2377-9_2 · Cited by 14 · see citing works
Himar1 Transposon for Efficient Random Mutagenesis in Aggregatibacter actinomycetemcomitans
Ding, Q., & Tan, K. S. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01842 · Cited by 14 · see citing works
Tho, N. P., Son, L. T., Tho, N. T., Cuong, B. D., Toan, H. P., Khánh, H. Q., & Thanh, N. H. (2021) · Microbiology · doi:10.1134/s0026261721040147 · Cited by 13 · see citing works
Xiao, L., Zhao, X., Lin, L., Mahsa, G. C., Ma, K., Zhang, C., Rui, X., & Li, W. (2024) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.4c04256 · Cited by 13 · see citing works
Ge, Z., Wang, D., Zhao, W., Wang, P., & Zhao, X. (2025) · Food Reviews International · doi:10.1080/87559129.2025.2467163 · Cited by 14 · see citing works
Novel imaging technologies for characterization of microbial extracellular polysaccharides
Lilledahl, M. B., & Stokke, B. T. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00525 · Cited by 13 · see citing works
Zhang, Y., Wu, Y., Zhao, X., Ye, Q., Cao, L., Liu, M., Gao, B., Niu, Q., Chen, N., Duan, Z., et al. (2025) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2025.07.028 · Cited by 12 · see citing works
Cell surface polysaccharides in the gut microbiota: occurrence, structure and role
Laplanche, V., Speciale, I., Castro, C. D., & Juge, N. (2025) · Gut Microbes · doi:10.1080/19490976.2025.2536082 · Cited by 12 · see citing works
Wang, K., Wang, Y., Gu, L., Yu, J., Liu, Q., Zhang, R., Liang, G., Chen, H., Gu, F., Liu, H., et al. (2024) · Microorganisms · doi:10.3390/microorganisms12040672 · Cited by 15 · see citing works
Dev, M. J., Mahajan, G., Warke, R. G., Warke, G. M., Patil, T. A., Satardekar, M. R., Dalvi, R. C., & Singhal, R. S. (2023) · International Microbiology · doi:10.1007/s10123-023-00399-1 · Cited by 12 · see citing works
Llamas-Arriba, M. G., Pérez-Ramos, A., Puertas, A. I., López, P., Dueñas, M. T., & Prieto, A. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02041 · Cited by 12 · see citing works
Comparative genomics of Lactobacillus johnsonii reveals extensive intraspecific genetic variation
Chen, K., Zhou, X., Zhao, J., Ross, R. P., Stanton, C., Chen, W., & Yang, B. K. (2023) · Food Bioscience · doi:10.1016/j.fbio.2023.103190 · Cited by 11 · see citing works
Karasu, E. N., & Ermiş, E. (2018) · Journal of Food Engineering · doi:10.1016/j.jfoodeng.2018.08.026 · Cited by 11 · see citing works
Özkan, E. R., Öztürk, H. İ., Demirci, T., & Akın, N. (2021) · LWT · doi:10.1016/j.lwt.2021.112077 · Cited by 11 · see citing works
Sengupta, D., Datta, S., & Biswas, D. (2019) · Journal of Basic Microbiology · doi:10.1002/jobm.201900080 · Cited by 11 · see citing works
Woo, S., Sung, J.-M., Park, J., Park, E. Y., & Park, E. Y. (2025) · Food Research International · doi:10.1016/j.foodres.2025.117523 · Cited by 10 · see citing works
Meng, F., Lyu, Y., Chen, X., Lü, F., Zhao, H., Lu, Y., Zhao, M., & Lu, Z. (2023) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.2c07880 · Cited by 9 · see citing works
Yang, S., Liu, L., Wang, J., Guo, S., Liu, G., Chen, X., Deng, X., Tu, M., Tao, Y., & Rao, Y. (2022) · Archives of Microbiology · doi:10.1007/s00203-022-03232-x · Cited by 9 · see citing works
Characterisation of dextran AP-27 produced by bee pollen isolate Lactobacillus kunkeei AP-27
Yılmaz, M. T., İspirli, H., Alidrisi, H. M., Taylan, O., & Dertli̇, E. (2023) · Process Biochemistry · doi:10.1016/j.procbio.2023.03.007 · Cited by 7 · see citing works
- Authors
- Dertli, E., Colquhoun, I. J., Gunning, A. P., Bongaerts, R. J., Le Gall, G., Bonev, B. B., Mayer, M. J., & Narbad, A.
- Affiliations
- Quadram Institute (GB)
- University of Nottingham (GB)
- Corresponding author
- Mayer, Melinda J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2013-11
- Volume
- 288
- Issue
- 44
- Pages
- 31938-31951
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC3814790
- OpenAlex
- W1964087334
- Cited by
- 134
- References
- 58
- Citations by year
- 2026: 13 · 2025: 13 · 2024: 4 · 2023: 13 · 2022: 11 · 2021: 10 · 2020: 14 · 2019: 13 · 2018: 10 · 2017: 11 · 2016: 4 · 2015: 14 · 2014: 4
- FWCI
- 10.85
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DSpace@Bayburt (submittedVersion, cc-by-nc-nd)
- UEA Digital Repository (University of East Anglia) (submittedVersion)
- Funders
- Biotechnology and Biological Sciences Research Council ((BB/J004529/1, BBS/E/F/00044453, BB/J004545/1)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Keywords
- exopolysaccharides, exopolysaccharide biosynthesis, heteropolymers, galactose substitution, O-acetylation, cell surface polysaccharides, biofilm formation
- MeSH terms
- Bacterial Proteins, Carbohydrate Conformation, Genes, Bacterial, Multigene Family, Glucosyltransferases, Lactobacillus, Mutation, Polysaccharides, Bacterial
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