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Cited by 206

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

The Cell Adhesion Protein P-selectin Glycoprotein Ligand-1 Is a Substrate for the Aspartyl Protease BACE1

Lichtenthaler, Stefan F., Dominguez, Diana-ines, Westmeyer, Gil G., Reiss, Karina, Haass, Christian, Saftig, Paul, De Strooper, Bart, Seed, Brian·2003·Journal of Biological Chemistry
DOI 10.1074/jbc.m303861200
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Abstract

The aspartyl protease BACE1 cleaves the amyloid precursor protein and the sialyltransferase ST6Gal I and is important in the pathogenesis of Alzheimer's disease. The normal function of BACE1 and additional physiological substrates have not been identified. Here we show that BACE1 acts on the P-selectin glycoprotein ligand 1 (PSGL-1), which mediates leukocyte adhesion in inflammatory reactions. In human monocytic U937 and human embryonic kidney 293 cells expressing endogenous or transfected BACE1, PSGL-1 was cleaved by BACE1 to generate a soluble ectodomain and a C-terminal transmembrane fragment. No evidence of the cleavage fragment was seen in primary cells derived from mice deficient in BACE1. By using deletion constructs and enzymatic deglycosylation of the C-terminal PSGL-1 fragments, the cleavage site in PSGL-1 was mapped to the juxtamembrane region within the ectodomain. In an in vitro assay BACE1 catalyzed the formation of the PSGL-1 products seen in vivo. The cleavage occurred at a Leu-Ser peptide bond as identified by mass spectrometry using a synthetic peptide. We conclude that PSGL-1 is an additional substrate for BACE1.

Overview

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Registry

Crossref · 10.1074/jbc.m303861200

Cites

2 of 2 references verified0 citation errors · 0 not checkable

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Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Sialyltransferase and PSGL-1, in addition to APP and its homologues APLP1 and 2, are the only other reported substrates of BACE.

    “Sialyltransferase and PSGL-1, in addition to APP and its homologues APLP1 and 2 are the only other reported substrates of BACE (6,43).”

    From The Low Density Lipoprotein Receptor-related Protein (LRP) Is a Novel β-Secretase (BACE1) Substrate (2005) · cites this paper as [43]

  • PSGL-1 is a BACE1 substrate that plays an important role in immune defenses by recruiting white blood cells to the site of infection.

    “P‐selectin glycoprotein ligand‐1 (PSGL‐1) is a BACE1 substrate that plays an important role in immune defenses by recruiting white blood cells to the site of infection.”

    From BACE1: More than just a β‐secretase (2022) · cites this paper as [25]

  • Nearly 70 BACE1 substrates have been identified to date.

    “through secretome enrichment experiments, nearly 70 BACE1 substrates have been identified to date.”

    From BACE1: More than just a β‐secretase (2022) · cites this paper as [25]

  • APP is a poor substrate for BACE1.

    “It is well acknowledged that APP is a poor substrate for BACE1”

    From BACE1: More than just a β‐secretase (2022) · cites this paper as [25]

Citations

Cites

Flagged first, then by citations

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Metadata

Authors
Lichtenthaler, S. F., Dominguez, D.-I., Westmeyer, G. G., Reiss, K., Haass, C., Saftig, P., De Strooper, B., & Seed, B.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2003-12
Volume
278
Issue
49
Pages
48713-48719
ISSN
0021-9258
DOI
10.1074/jbc.m303861200
Cited by
206
References
2
Language
en
Licence

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