CoChat
Sign Up
Cited by 334

The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

HsAtg4B/HsApg4B/Autophagin-1 Cleaves the Carboxyl Termini of Three Human Atg8 Homologues and Delipidates Microtubule-associated Protein Light Chain 3- and GABAA Receptor-associated Protein-Phospholipid Conjugates

Tanida, Isei, Sou, Yu-shin, Ezaki, Junji, Minematsu-Ikeguchi, Naoko, Ueno, Takashi, Kominami, Eiki·2004·Journal of Biological Chemistry
DOI 10.1074/jbc.m401461200
Open this paper in CoChat — free account

Abstract

In yeast, Atg4/Apg4 is a unique cysteine protease responsible for the cleavage of the carboxyl terminus of Atg8/Apg8/Aut7, a reaction essential for its lipidation during the formation of autophagosomes. However, it is still unclear whether four human Atg4 homologues cleave the carboxyl termini of the three human Atg8 homologues, microtubule-associated protein light chain 3 (LC3), GABARAP, and GATE-16. Using a cell-free system, we found that HsAtg4B, one of the human Atg4 homologues, cleaves the carboxyl termini of these three Atg8 homologues. In contrast, the mutant HsAtg4B(C74A), in which a predicted active site Cys(74) was changed to Ala, lacked proteolytic activity, indicating that Cys(74) is essential for the cleavage activity of cysteine protease. Using phospholipase D, we showed that the modified forms of endogenous LC3 and GABARAP are lipidated and therefore were designated LC3-PL and GABARAP-PL. When purified glutathione S-transferase-tagged HsAtg4B was incubated in vitro with a membrane fraction enriched with endogenous LC3-PL and GABARAP-PL, the mobility of LC3-PL and GABARAP-PL was changed to those of the unmodified proteins. These mobility shifts were not seen when Cys(74) of HsAtg4B was changed to Ala. Overexpression of wild-type HsAtg4B decreased the amount of LC3-PL and GABARAP-PL and increased the amount of unmodified endogenous LC3 and GABARAP in HeLa cells. Expression of CFP-tagged HsAtg4B (CFP-HsAtg4B) and YFP-tagged LC3 in HeLa cells under starvation condi…

Overview

Integrity signals

Registry

Crossref · 10.1074/jbc.m401461200

Cites

4 of 4 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Commercial antibodies for GABARAPL1 also recognize GABARAP, which might lead to misinterpretation of experiments, particularly those using immunohistochemical techniques.

    “Furthermore, commercial antibodies for GABARAPL1 also recognize GABARAP [33,195], which might lead to misinterpretation of experiments, in particular those using immunohistochemical techniques.”

    From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [195]

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 31

  1. Huang, 2002, Cell Struct. Funct., 27, 409, 10.1247/csf.27.409 · DOI
  2. Klionsky, 2003, Dev. Cell, 5, 539, 10.1016/S1534-5807(03)00296-X · DOI
  3. Ichimura, 2000, Nature, 408, 488, 10.1038/35044114 · DOI
  4. Kirisako, 2000, J. Cell Biol., 151, 263, 10.1083/jcb.151.2.263 · DOI
  5. Kim, 1999, Mol. Biol. Cell, 10, 1337, 10.1091/mbc.10.5.1337 · DOI
  6. Shintani, 1999, EMBO J., 18, 5234, 10.1093/emboj/18.19.5234 · DOI · Paper page
  7. Tanida, 1999, Mol. Biol. Cell, 10, 1367, 10.1091/mbc.10.5.1367 · DOI
  8. Yuan, 1999, Mol. Biol. Cell, 10, 1353, 10.1091/mbc.10.5.1353 · DOI
  9. Reggiori, 2002, Eukaryot. Cell, 1, 11, 10.1128/EC.01.1.11-21.2002 · DOI
  10. Kadowaki, 2003, J. Nutr., 133, 2052S, 10.1093/jn/133.6.2052S · DOI
  11. Yoshimori, 2004, Biochem. Biophys. Res. Commun., 313, 453, 10.1016/j.bbrc.2003.07.023 · DOI
  12. Raught, 2001, Proc. Natl. Acad. Sci. U. S. A., 98, 7037, 10.1073/pnas.121145898 · DOI
  13. Saftig, 2001, Trends Mol. Med., 7, 37, 10.1016/S1471-4914(00)01868-2 · DOI
  14. Thummel, 2001, BioEssays, 23, 677, 10.1002/bies.1096 · DOI
  15. Larsen, 2002, Histol. Histopathol., 17, 897
  16. Lockshin, 2002, Curr. Opin. Cell Biol., 14, 727, 10.1016/S0955-0674(02)00383-6 · DOI
  17. Tolkovsky, 2002, Biochimie (Paris), 84, 233, 10.1016/S0300-9084(02)01371-8 · DOI
  18. Ogier-Denis, 2003, Biochim. Biophys. Acta, 1603, 113
  19. Mizushima, 2001, J. Cell Biol., 152, 657, 10.1083/jcb.152.4.657 · DOI · Paper page
  20. Tanida, 2001, J. Biol. Chem., 276, 1701, 10.1074/jbc.C000752200 · DOI
  21. Tanida, 2002, J. Biol. Chem., 277, 13739, 10.1074/jbc.M200385200 · DOI
  22. Mann, 1994, J. Biol. Chem., 269, 11492, 10.1016/S0021-9258(19)78150-2 · DOI
  23. Mann, 1996, J. Neurosci. Res., 43, 535, 10.1002/(SICI)1097-4547(19960301)43:5<535::AID-JNR3>3.0.CO;2-J · DOI
  24. Wang, 1999, Nature, 397, 69, 10.1038/16264 · DOI
  25. Sagiv, 2000, EMBO J., 19, 1494, 10.1093/emboj/19.7.1494 · DOI
  26. Hemelaar, 2003, J. Biol. Chem., 278, 51841, 10.1074/jbc.M308762200 · DOI · Paper page
  27. Scherz-Shouval, 2003, J. Biol. Chem., 278, 14053, 10.1074/jbc.M212108200 · DOI
  28. Tanida, 2003, Biochem. Biophys. Res. Commun., 300, 637, 10.1016/S0006-291X(02)02907-8 · DOI
  29. Marino, 2003, J. Biol. Chem., 278, 3671, 10.1074/jbc.M208247200 · DOI
  30. Thumm, 2001, Mol. Genet. Genomics, 266, 657, 10.1007/s004380100585 · DOI
  31. Nemoto, 2003, J. Biol. Chem., 278, 39517, 10.1074/jbc.M300550200 · DOI

Cited by

Sorted by citations

Metadata

Authors
Tanida, I., Sou, Y.-S., Ezaki, J., Minematsu-Ikeguchi, N., Ueno, T., & Kominami, E.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2004-08
Volume
279
Issue
35
Pages
36268-36276
ISSN
0021-9258
DOI
10.1074/jbc.m401461200
Cited by
334
References
4
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review