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Cited by 235Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyFood and Agricultural Sciences

The nutritive value of rumen micro-organisms in ruminants: 1. Large-scale isolation and chemical composition of rumen micro-organisms

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Storm, E., Ørskov, E. R.·1983·British Journal Of Nutrition
DOI 10.1079/bjn19830114
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Abstract

A method is described whereby a large quantity of rumen microbial dry matter of high purity was isolated from whole rumen contents obtained from abattoirs, by means of a continuous process of one filtration through four sieves followed by three differential centrifugations. The contents of ash, carbohydrate, lipid, nitrogen, RNA, DNA and individual amino acids of the three centrifugal fractions are given and compared with values summarized from more than sixty published reports on the chemical composition of rumen micro-organisms isolated from both whole rumen contents and pure cultures. The amino acid composition of isolated rumen micro-organisms, in particular that of the bacteria, was found to be remarkably constant.

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  • Microbial cell biomass is utilized as the primary origin of protein and amino acids by host animals.

    “Besides, microbial cell biomass is also utilized as the primary origin of protein and amino acids by host animals (3).”

    From Phytogenic Additives Can Modulate Rumen Microbiome to Mediate Fermentation Kinetics and Methanogenesis Through Exploiting Diet–Microbe Interaction (2020) · cites this paper as [3]

    The opening section reports that, for a wide range of normal diets, 60–85% of the protein passing to a ruminant’s small intestine is of microbial origin, and that rumen microbes synthesize amino acids. This supports the claim for ruminants, but not its unqualified extension to host animals generally. The supplied passage does not give a page number. (LLM, conf=0.83)

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Research works printed but not linked: 30

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  21. Ty. Ruckebusch and P. Thivend, editors]. Lancaster: MTP Press Ltd. Nutrition 24, 157-177.
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  30. E X P L A N A T I O N OF P L A T E Plate 1. Micrographs ( x 1500) of rumen fluid after various isolation treatments. (a) Rumen fluid after filtration through a 50 pm sieve, showing a mixture of bacteria, protozoa and dietary matter; (b) supernatant fraction after extraction of fraction no. 1 by centrifugation at 1200 g for 4 min, showing a large number of bacteria but virtually no dietary matter and no protozoa; (c) supernatant fraction after extraction of fraction no. 2 by centrifugation at 19500g for 8 min, showing a greatly reduced number of bacteria; (d) supernatant fraction after extraction of fraction no. 2 by centrifugation at 19500 g for 15 min, showing only very few organisms. Printed in Great Britain https://doi.org/10.1079/BJN19830114 Published online by Cambridge University Press

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Authors
Storm, E., & Ørskov, E. R.
Venue
British Journal Of Nutrition
Publisher
Cambridge University Press (CUP)
Published
1983-09
Volume
50
Issue
2
Pages
463-470
ISSN
  • 0007-1145 (ISSN-L)
  • 1475-2662
DOI
10.1079/bjn19830114
OpenAlex
W2397518067
Cited by
229
References
36
Citations by year
2026: 2 · 2025: 9 · 2024: 11 · 2023: 3 · 2022: 4 · 2021: 8 · 2020: 4 · 2019: 6 · 2018: 5 · 2017: 10 · 2016: 6 · 2015: 4 · 2014: 6 · 2013: 6 · 2012: 8
FWCI
10.79
Type
journal article
Language
en
Open access
Licence
www.cambridge.org/core/terms (unspecified, 8590 d)
Locations
  • British Journal Of Nutrition (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
Keywords
rumen microbiota, ruminants, rumen bacteria, microbial protein, amino acid composition, rumen contents, chemical composition
MeSH terms
Amino Acids, Animals, Animal Nutritional Physiological Phenomena, Bacteria, Carbohydrates, Cattle, Lipids, Nitrogen, Nutritive Value, Rumen, Sheep, Eukaryota

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