Effect of temperature on synthesis of clavulanic acid and impurity substance G during fermentation by Streptomyces clavuligerus
Abstract
Effect of temperature on synthesis of Clavulanic acid (CA) and impurity substance G during fermentation by Streptomyces clavuligerus were investigated. Results show that fermentation at 24 °C is the most favorable for CA synthesis though the fermentation duration was 20–30 hours longer than fermentation at 26 and 28 °C. Meanwhile, the impurity substance G was only 110 mg/L in the end broth of fermentation at 24 °C, which was significantly lower than 148 and 180 mg/L of fermentation at 26 and 28 °C, respectively. Correlation of specific growth rate and CA synthesis was statistically analyzed based on data of 10 batches of industrial fermentation. Two temperature-shift strategies were investigated in 50 L fermenter. Fermentation with 26–24 °C temperature strategy achieved 5097 mg/L CA titer, meanwhile the fermentation duration was shortened 24 hours comparing with fermentation at constant 24 °C. Fermentation with 26–24 °C control strategy was validated in a 60 m3 industrial fermenter, in which 4960 mg/L of CA was achieved while impurity G substance was decreased to titer 65 mg/L from 200 to 300 mg/L of normal production.
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Cited by
Still being checked.
- Authors
- Feng, T., Zhao, J., Bai, Y.-F., Chu, J., Wang, Y.-H., & Zhuang, Y.-P.
- Affiliations
- East China University of Science and Technology (CN)
- China National Pharmaceutical Group Corporation (China) (CN)
- State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People’s Republic China
- Corresponding author
- Chu, Ju
- Venue
- Preparative Biochemistry & Biotechnology
- Publisher
- Informa UK Limited
- Published
- 2021-12-06
- Volume
- 52
- Issue
- 8
- Pages
- 937-941
- ISSN
- 1082-6068 (ISSN-L)
- 1532-2297
- OpenAlex
- W4200344345
- Semantic Scholar
- d6e3703768cdd27173243d9b494cea0c14fa781b
- Cited by
- 3
- References
- 21
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 1
- FWCI
- 0.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Preparative Biochemistry & Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Shan-Xi provincial Scientific & Technology Transformation (201804D121004)
- Topics
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- clavulanic acid, Streptomyces clavuligerus, fermentation temperature, specific growth rate, fermentation time, industrial fermentation, antibiotic production
- MeSH terms
- Fermentation, Streptomyces, Temperature, Clavulanic Acid
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