Cited by 104Gut microbiota and healthMicrobial Community Ecology and PhysiologyOral microbiology and periodontitis research

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Cultured microbes represent a substantial fraction of the human and mouse gut microbiota

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Lagkouvardos, Ilias, Overmann, Jörg, Clavel, Thomas·2017·Gut Microbes

DOI 10.1080/19490976.2017.1320468PMC PMC5628658

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Abstract

During the last 15 years, molecular techniques have been preferred over culture-based approaches for the study of mammalian gut microbiota, i.e. the communities of microorganisms dwelling in the intestine of mammals. The main reason is the belief that the majority of gut bacteria, especially strict anaerobes, escape cultivation. Despite numerous such statements in publications, the literature does not provide a clear overview on the subject. In the present manuscript, we highlight recent work on the cultivation of bacteria from the intestine of mammals, review the literature and provide novel data pertaining to cultured fractions of mammalian gut microbiota. These data show that, despite marked inter-sample variations, 35 to 65% of molecular species detected by sequencing have representative strains in culture.

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See all 104

Authors: Lagkouvardos, I., Overmann, J., & Clavel, T.
Affiliations: Technical University of Munich (DE)
German Center for Infection Research (DE)
Universitätsklinikum Aachen (DE)
Corresponding author: Clavel, Thomas
ORCID: Lagkouvardos, Ilias
Overmann, Jörg
Clavel, Thomas
Venue: Gut Microbes
Publisher: Informa UK Limited
Published: 2017-04-18
Volume: 8
Issue: 5
Pages: 493-503
ISSN: 1949-0976 (ISSN-L)
1949-0984
DOI: 10.1080/19490976.2017.1320468
PMC: PMC5628658
OpenAlex: W2606877093
Cited by: 104
References: 80
Citations by year: 2026: 7 · 2025: 9 · 2024: 7 · 2023: 11 · 2022: 10 · 2021: 9 · 2020: 19 · 2019: 12 · 2018: 14 · 2017: 6
FWCI: 1.31
Type: journal article
Language: en
Open access: gold
doi.org
Locations: Gut Microbes (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
RWTH Publications (RWTH Aachen) (submittedVersion)
Funders: Deutsche Forschungsgemeinschaft (SPP-1656)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Keywords: gut microbiota, bacterial culture, human gut microbiome, mouse gut microbiome, obligate anaerobes, representative strain
MeSH terms: Gastrointestinal Microbiome, Animals, Bacteria, Humans, Mice

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Cultured microbes represent a substantial fraction of the human and mouse gut microbiota

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