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Acetaldehyde production by Rothia mucilaginosa isolates from patients with oral leukoplakia

Amer, Abdrazak, Whelan, Aine, Al-Hebshi, Nezar N., Healy, Claire M., Moran, Gary P.·2020·Journal of Oral Microbiology
DOI 10.1080/20002297.2020.1743066PMC PMC7170386
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Figure 1. (a) Graphic representation of ALDH coding capacity in different taxonomic groups of oral bacteria, investigated using the PATRIC database. The percentage of strains with a predicted ALDH encoding gene is indicated and highlighted in red. Other Firmicutes includes Granulicatella spp. (n = 6) and Gemella spp. (n = 4). Fusobacteria includes Fusobacterium spp. (n = 15) and Leptotrichia spp. (n = 15). Bacteroidetes includes Porphyromonas spp. (n = 71), Prevotella spp, (n = 66) and Tannerella spp. (n = 3). (b) Plot generated in LEfSe showing the increased abundance of acetaldehyde dehydrogenase enzyme (EC 1.2.1.10) encoding genes in the metagenomes of samples from healthy NC mucosa compared to OLK (P = 0.008). OLK are categorized as in mild to moderate dysplasia (red) and severe dysplasia (green). Straight and dotted lines correspond to mean and median values, respectively
Figure 1. (a) Graphic representation of ALDH coding capacity in different taxonomic groups of oral bacteria, investigated using the PATRIC database. The percentage of strains with a predicted ALDH encoding gene is indicated and highlighted in red. Other Firmicutes includes Granulicatella spp. (n = 6) and Gemella spp. (n = 4). Fusobacteria includes Fusobacterium spp. (n = 15) and Leptotrichia spp. (n = 15). Bacteroidetes includes Porphyromonas spp. (n = 71), Prevotella spp, (n = 66) and Tannerella spp. (n = 3). (b) Plot generated in LEfSe showing the increased abundance of acetaldehyde dehydrogenase enzyme (EC 1.2.1.10) encoding genes in the metagenomes of samples from healthy NC mucosa compared to OLK (P = 0.008). OLK are categorized as in mild to moderate dysplasia (red) and severe dysplasia (green). Straight and dotted lines correspond to mean and median values, respectivelyCC BY · PubMed Central
Figure 2. Growth of oral bacteria in BHI at 37°C in the presence of ethanol (0%, 2% and 4% v/v). Plots are the results of three separate replicate experiments
Figure 2. Growth of oral bacteria in BHI at 37°C in the presence of ethanol (0%, 2% and 4% v/v). Plots are the results of three separate replicate experimentsCC BY · PubMed Central
Figure 3. (a) Aldehyde production by representative oral microorganisms in the presence of 10 mM and 100 mM ethanol. Aldehyde was detected using a colorimetric assay. Data is the average of three separate experiments. Red asterisk indicates different axis scale. (b) Aldehyde production by clinical isolates of R. mucilaginosa in the presence of 100 mM ethanol. Red: >100 µM; Orange: 30–100 µM; yellow: < 20 µM. (c) Comparison of aldehyde and acetaldehyde measurements by colorimetric and GC-MS analysis following incubation of strains in 100 mM ethanol
Figure 3. (a) Aldehyde production by representative oral microorganisms in the presence of 10 mM and 100 mM ethanol. Aldehyde was detected using a colorimetric assay. Data is the average of three separate experiments. Red asterisk indicates different axis scale. (b) Aldehyde production by clinical isolates of R. mucilaginosa in the presence of 100 mM ethanol. Red: >100 µM; Orange: 30–100 µM; yellow: < 20 µM. (c) Comparison of aldehyde and acetaldehyde measurements by colorimetric and GC-MS analysis following incubation of strains in 100 mM ethanolCC BY · PubMed Central
Figure 4. (a) Showing ACH induced oxidative stress in TR146 oral keratinocytes. Cells were incubated with ACH (25 µM – 100 µM) in 96 well plates. 30 mM H2O2 was used as positive control and medium containing PBS as a negative control. Reactive oxygen species (ROS) were detected by measuring DCF-DA fluorescence in a microplate reader (Genios, Tecan) in the presence and absence of N-acetylcysteine (NAC). Results are the average of three separate experiments. (b) Showing fluorescence in TR146 cells stained with DCF-DA to detect ROS (green) and Hoechst to detect nuclei (blue). Cells were preincubated in 100 µM of DCF-DA with or without NAC. Cells were then exposed to stress (50 or 100 µM ACH or 30 mM H2O2) and visualized using a Zeiss epifluorescence microscope
Figure 4. (a) Showing ACH induced oxidative stress in TR146 oral keratinocytes. Cells were incubated with ACH (25 µM – 100 µM) in 96 well plates. 30 mM H2O2 was used as positive control and medium containing PBS as a negative control. Reactive oxygen species (ROS) were detected by measuring DCF-DA fluorescence in a microplate reader (Genios, Tecan) in the presence and absence of N-acetylcysteine (NAC). Results are the average of three separate experiments. (b) Showing fluorescence in TR146 cells stained with DCF-DA to detect ROS (green) and Hoechst to detect nuclei (blue). Cells were preincubated in 100 µM of DCF-DA with or without NAC. Cells were then exposed to stress (50 or 100 µM ACH or 30 mM H2O2) and visualized using a Zeiss epifluorescence microscopeCC BY · PubMed Central

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Research works printed but not linked: 36

  1. 10.1371/journal.pone.0098741 · DOI
  2. 10.1038/s41598-017-02079-3 · DOI
  3. 10.3402/jom.v8.32762 · DOI
  4. 10.3402/jom.v2i0.5780 · DOI
  5. 10.1080/13556210020077028 · DOI
  6. 10.3402/jom.v2i0.5195 · DOI
  7. 10.1093/oxfordjournals.alcalc.a008191 · DOI
  8. International Agency for Research on Cancer. Acetaldehyde. IARC monographs on the evaluation of carcinogenic risks to humans. Vol. 71 Pt 2, World Health Organization, IARC press, Lyon, France; 1999. p. 319–335.
  9. 10.1152/ajpgi.00103.2014 · DOI
  10. 10.3390/cancers10010020 · DOI
  11. 10.1016/S1368-8375(00)00076-2 · DOI
  12. 10.1093/carcin/21.4.663 · DOI
  13. 10.1016/j.oooo.2013.02.009 · DOI
  14. 10.1073/pnas.1102444108 · DOI
  15. 10.1128/JB.02450-14 · DOI
  16. 10.1128/AEM.02035-18 · DOI
  17. 10.1002/1097-0215(20001101)88:3<342::AID-IJC4>3.0.CO;2-I · DOI
  18. 10.1099/mic.0.066258-0 · DOI
  19. 10.1016/j.oraloncology.2009.08.002 · DOI
  20. Gainza-Cirauqui M, 2012, J Oral Pathol Med, 42, 249
  21. 10.1111/odi.12341 · DOI
  22. 10.1016/j.oraloncology.2006.02.005 · DOI
  23. 10.1080/20002297.2018.1492316 · DOI
  24. 10.1128/genomeA.01122-15 · DOI
  25. 10.1016/j.mimet.2012.09.011 · DOI
  26. 10.3389/fmicb.2017.02391 · DOI · Paper page
  27. 10.3945/ajcn.115.116244 · DOI · Paper page
  28. 10.1016/j.oraloncology.2018.12.003 · DOI
  29. 10.1093/nar/gkw1017 · DOI
  30. 10.1038/nbt.2676 · DOI · Paper page
  31. 10.1128/AEM.01541-09 · DOI
  32. 10.1186/gb-2011-12-6-r60 · DOI · Paper page
  33. 10.1099/00221287-143-2-405 · DOI · Paper page
  34. 10.1111/omi.12151 · DOI · Paper page
  35. 10.1177/0022034518767118 · DOI
  36. 10.1371/journal.pgen.1005413 · DOI

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Metadata

Authors
Amer, A., Whelan, A., Al-Hebshi, N. N., Healy, C. M., & Moran, G. P.
Affiliations
  • Division of Oral Biosciences, School of Dental Science, Trinity College Dublin, Dublin Dental University Hospital, Dublin, Ireland
  • School of Chemical and Pharmaceutical Sciences, Technological University, Dublin, Ireland
  • Oral Microbiome Research Laboratory, Maurice H. Kornberg School of Dentistry, Temple University, Philadelphia, PA, USA
  • Division of Oral and Maxillofacial Surgery, Oral Medicine and Oral Pathology, School of Dental Science, Trinity College Dublin, Dublin Dental University Hospital, Dublin, Ireland
ORCID
Moran, Gary P.
Venue
Journal of Oral Microbiology
Publisher
Informa UK Limited
Published
2020-01-01
Volume
12
Issue
1
Pages
1743066
ISSN
2000-2297
DOI
10.1080/20002297.2020.1743066
PMC
PMC7170386
Cited by
43
References
3
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (unspecified, 311 d)
Funders
  • Libyan Ministry of Higher Education and Scientific Research (9960)
  • Irish Health Research Board (ILP-POR-2019-030)

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CoChat. (2026). Citation check: Acetaldehyde production by Rothia mucilaginosa isolates from patients with oral leukoplakia. https://app.cochat.ai/papers/10.1080/20002297.2020.1743066
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Amer, A., Whelan, A., Al-Hebshi, N. N., Healy, C. M., & Moran, G. P. (2020). Acetaldehyde production by Rothia mucilaginosa isolates from patients with oral leukoplakia. Journal of Oral Microbiology. https://doi.org/10.1080/20002297.2020.1743066