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Cited by 45CRISPR and Genetic EngineeringGenetically Modified Organisms ResearchPlant Genetic and Mutation Studies

The DOI matches its Crossref record; 252 of the 252 references it cites were found in a registry or an index.

Putting CRISPR-Cas system in action: a golden window for efficient and precise genome editing for crop improvement

Open access
Tariq, Arooj, Mushtaq, Muntazir, Yaqoob, Huwaida, Bhat, Basharat Ahmad, Zargar, Sajad Majeed, Raza, Ali, Ali, Sajad, Charagh, Sidra, Mubarik, Muhammad Salman, Zaman, Qamar U, Prasad, PV Vara, Mir, Rakeeb Ahmad·2023·GM Crops & Food
DOI 10.1080/21645698.2023.2219111PMC PMC10251801
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Abstract

The daunting task of feeding an ever-growing population is an immense challenge for the contemporary scientific community, especially in view of the rapidly changing climate throughout the world. Amidst these threatening crises, we witness rapid development in genome editing (GE) technologies, revolutionizing the field of applied genomics and molecular breeding. Various GE tools have been developed during the last two decades, but the CRISPR/Cas system has most recently made a significant impact on crop improvement. The major breakthroughs of this versatile toolbox are genomic modifications like single base-substitutions, multiplex GE, gene regulation, screening mutagenesis, and enhancing the breeding of wild crop plants. Previously, this toolbox was used to modify genes related to significant traits such as biotic/abiotic resistance/tolerance, post-harvest traits, nutritional regulation, and to address self-incompatibility analysis-related challenges. In the present review, we have demonstrated the functional dynamics of CRISPR-based GE and its applicability in targeting genes to accomplish novel editing of crops. The compiled knowledge will provide a solid foundation for highlighting the primary source for applying CRISPR/Cas as a toolbox for enhancing crops, to achieve food and nutritional security.

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Crossref · 10.1080/21645698.2023.2219111

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Figures

Figure 1. Illustration for developing superior and elite crop plants resilient to biotic and abiotic stress factors, and enhancing the quality of crops by using CRISPR/Cas systems through single base substitution, multiplex gene editing, gene knockout, regulation of gene transcription, and enhancing and screening mutagenesis.
Figure 1. Illustration for developing superior and elite crop plants resilient to biotic and abiotic stress factors, and enhancing the quality of crops by using CRISPR/Cas systems through single base substitution, multiplex gene editing, gene knockout, regulation of gene transcription, and enhancing and screening mutagenesis.CC BY · PubMed Central
Figure 2. Schematics show the mechanistic workflow of the CRISPR/Cas system and its precise catalysis by inducing editing through NHEJ or HDR, employed to improve crops.
Figure 2. Schematics show the mechanistic workflow of the CRISPR/Cas system and its precise catalysis by inducing editing through NHEJ or HDR, employed to improve crops.CC BY · PubMed Central
Figure 3. Ways toward precise editing: The prime editing mechanism involves the formation of a complex that comprises pegRNA to yield single-strand nick at a precise position in the target DNA PAM sequences, followed by the polymerization of newly edited DNA by reverse transcriptase by using pegRNA as a template. Subsequently, the sgRNA is employed to form the nick on the non-modified DNA strand to initiate repair-based insertion of editing in both the DNA strands. Base editied nCas9 is the preferred nuclease employed in dbase editing to generate single-stranded nick in the target DNA stranded, aided by gRNA using base deaminase to accomplish a single or more preferred nucleotide base exchanges.
Figure 3. Ways toward precise editing: The prime editing mechanism involves the formation of a complex that comprises pegRNA to yield single-strand nick at a precise position in the target DNA PAM sequences, followed by the polymerization of newly edited DNA by reverse transcriptase by using pegRNA as a template. Subsequently, the sgRNA is employed to form the nick on the non-modified DNA strand to initiate repair-based insertion of editing in both the DNA strands. Base editied nCas9 is the preferred nuclease employed in dbase editing to generate single-stranded nick in the target DNA stranded, aided by gRNA using base deaminase to accomplish a single or more preferred nucleotide base exchanges.CC BY · PubMed Central
Figure 4. Engineering crops for disease resistance. Schematics showing the examples of genes edited by the CRISPR/Cas system to enhance the resistance to bacteria, fungus, and virus..
Figure 4. Engineering crops for disease resistance. Schematics showing the examples of genes edited by the CRISPR/Cas system to enhance the resistance to bacteria, fungus, and virus..CC BY · PubMed Central

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Citations

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Research works printed but not linked: 1

  1. Kumar A. Application, Regulation, Ethical Concerns and Governance of Genome-Editing Technologies: an Overview. Asian Biotechnology & Development Review. 2019;21.

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Metadata

Authors
Tariq, A., Mushtaq, M., Yaqoob, H., Bhat, B. A., Zargar, S. M., Raza, A., Ali, S., Charagh, S., Mubarik, M. S., Zaman, Q. U., Prasad, P. V., & Mir, R. A.
Affiliations
  • Baba Ghulam Shah Badshah University (IN)
  • Shoolini University (IN)
  • Cluster University Srinagar (IN)
  • Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (IN)
  • Fujian Agriculture and Forestry University (CN)
  • Yeungnam University (KR)
  • Chinese Academy of Agricultural Sciences (CN)
  • University of Narowal (PK)
  • Hainan University (CN)
  • Kansas State University (US)
  • Central University of Kashmir (IN)
Corresponding author
  • Zargar, Sajad Majeed
  • Raza, Ali
  • Mir, Rakeeb Ahmad
ORCID
Venue
GM Crops & Food
Publisher
Informa UK Limited
Published
2023-06-08
Volume
14
Issue
1
Pages
1-27
ISSN
  • 2164-5698 (ISSN-L)
  • 2164-5701
DOI
10.1080/21645698.2023.2219111
PMC
PMC10251801
OpenAlex
W4379769205
Cited by
45
References
252
Citations by year
2026: 12 · 2025: 18 · 2024: 10 · 2023: 5
FWCI
1.19
Type
journal article
Language
en
Open access
Licence
Locations
  • GM crops & food (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
no external funding
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
Keywords
CRISPR/Cas genome editing, crop improvement, plant breeding, single base substitution, multiplex genome editing, gene expression regulation, mutagenesis screening, nutritional properties, self-incompatibility, food security
MeSH terms
Plant Breeding, Gene Editing, Crops, Agricultural, Genome, Plant, Plants, Genetically Modified, CRISPR-Cas Systems

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